MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

Abstract Insulin-producing cells (IPCs) derived from a patient’s own stem cells offer great potential for autologous transplantation in diabetic patients. However, the limited survival of engrafted cells remains a bottleneck in the application of this strategy. The present study aimed to investigate...

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Autores principales:	Chunlin Zou, Yi Lu, Xiahong Teng, Shuyan Wang, Xiaoting Sun, Fen Huang, Guannan Shu, Xin Huang, Hongwei Guo, Zhiguo Chen, Jian Zhang, Yu Alex Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9b45fa05cd54585a97973cc2765bcbb
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spelling	oai:doaj.org-article:c9b45fa05cd54585a97973cc2765bcbb2021-12-02T16:06:18ZMRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys10.1038/s41598-017-02775-02045-2322https://doaj.org/article/c9b45fa05cd54585a97973cc2765bcbb2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02775-0https://doaj.org/toc/2045-2322Abstract Insulin-producing cells (IPCs) derived from a patient’s own stem cells offer great potential for autologous transplantation in diabetic patients. However, the limited survival of engrafted cells remains a bottleneck in the application of this strategy. The present study aimed to investigate whether nanoparticle-based magnetic resonance (MR) tracking can be used to detect the loss of grafted stem cell-derived IPCs in a sensitive and timely manner in a diabetic monkey model. Pancreatic progenitor cells (PPCs) were isolated from diabetic monkeys and labeled with superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-labeled cells presented as hypointense signals on MR imaging (MRI). The labeling procedure did not affect the viability or IPC differentiation of PPCs. Importantly, the total area of the hypointense signal caused by SPION-labeled IPCs on liver MRI decreased before the decline in C-peptide levels after autotransplantation. Histological analysis revealed no detectable immune response to the grafts and many surviving insulin- and Prussian blue-positive cell clusters on liver sections at one year post-transplantation. Collectively, this study demonstrates that SPIO nanoparticles can be used to label stem cells for noninvasive, sensitive, longitudinal monitoring of stem cell-derived IPCs in large animal models using a conventional MR imager.Chunlin ZouYi LuXiahong TengShuyan WangXiaoting SunFen HuangGuannan ShuXin HuangHongwei GuoZhiguo ChenJian ZhangYu Alex ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunlin Zou Yi Lu Xiahong Teng Shuyan Wang Xiaoting Sun Fen Huang Guannan Shu Xin Huang Hongwei Guo Zhiguo Chen Jian Zhang Yu Alex Zhang MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
description	Abstract Insulin-producing cells (IPCs) derived from a patient’s own stem cells offer great potential for autologous transplantation in diabetic patients. However, the limited survival of engrafted cells remains a bottleneck in the application of this strategy. The present study aimed to investigate whether nanoparticle-based magnetic resonance (MR) tracking can be used to detect the loss of grafted stem cell-derived IPCs in a sensitive and timely manner in a diabetic monkey model. Pancreatic progenitor cells (PPCs) were isolated from diabetic monkeys and labeled with superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-labeled cells presented as hypointense signals on MR imaging (MRI). The labeling procedure did not affect the viability or IPC differentiation of PPCs. Importantly, the total area of the hypointense signal caused by SPION-labeled IPCs on liver MRI decreased before the decline in C-peptide levels after autotransplantation. Histological analysis revealed no detectable immune response to the grafts and many surviving insulin- and Prussian blue-positive cell clusters on liver sections at one year post-transplantation. Collectively, this study demonstrates that SPIO nanoparticles can be used to label stem cells for noninvasive, sensitive, longitudinal monitoring of stem cell-derived IPCs in large animal models using a conventional MR imager.
format	article
author	Chunlin Zou Yi Lu Xiahong Teng Shuyan Wang Xiaoting Sun Fen Huang Guannan Shu Xin Huang Hongwei Guo Zhiguo Chen Jian Zhang Yu Alex Zhang
author_facet	Chunlin Zou Yi Lu Xiahong Teng Shuyan Wang Xiaoting Sun Fen Huang Guannan Shu Xin Huang Hongwei Guo Zhiguo Chen Jian Zhang Yu Alex Zhang
author_sort	Chunlin Zou
title	MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
title_short	MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
title_full	MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
title_fullStr	MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
title_full_unstemmed	MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
title_sort	mri tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c9b45fa05cd54585a97973cc2765bcbb
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MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

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