Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains

ABSTRACT Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Craig M. Stephens, Sheila Adams-Sapper, Manraj Sekhon, James R. Johnson, Lee W. Riley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Escherichia coli ExPEC ST95 antibiotic resistance genomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c9b48a7644a248d68eb79836b1b10fd1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c9b48a7644a248d68eb79836b1b10fd1
record_format	dspace
spelling	oai:doaj.org-article:c9b48a7644a248d68eb79836b1b10fd12021-11-15T15:21:46ZGenomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains10.1128/mSphere.00390-162379-5042https://doaj.org/article/c9b48a7644a248d68eb79836b1b10fd12017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00390-16https://doaj.org/toc/2379-5042ABSTRACT Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other pandemic ExPEC lineages. We took a genomic approach to identify factors that may underlie reduced resistance. We fully assembled genomes for four ST95 isolates representing the four major fimH-based lineages within ST95 and also analyzed draft-level genomes from another 82 ST95 isolates, largely from the western United States. The fully assembled genomes of antibiotic-resistant isolates carried resistance genes exclusively on large (>90-kb) IncFIB/IncFII plasmids. These replicons were common in the draft genomes as well, particularly in antibiotic-resistant isolates, but we also observed multiple instances of a smaller (8.3-kb) ampicillin resistance plasmid that had been previously identified in Salmonella enterica. Among ST95 isolates, pansusceptibility to antibiotics was significantly associated with the fimH6 lineage and the presence of homologs of the previously identified 114-kb IncFIB/IncFII plasmid pUTI89, both of which were also associated with reduced carriage of other plasmids. Potential mechanistic explanations for lineage- and plasmid-specific effects on the prevalence of antibiotic resistance within the ST95 group are discussed. IMPORTANCE Antibiotic resistance in bacterial pathogens is a major public health concern. This work was motivated by the observation that only a small proportion of ST95 isolates, a major pandemic lineage of extraintestinal pathogenic E. coli, have acquired antibiotic resistance, in contrast to many other pandemic lineages. Understanding bacterial genetic factors that may prevent acquisition of resistance could contribute to the development of new biological, medical, or public health strategies to reduce antibiotic-resistant infections.Craig M. StephensSheila Adams-SapperManraj SekhonJames R. JohnsonLee W. RileyAmerican Society for MicrobiologyarticleEscherichia coliExPECST95antibiotic resistancegenomicsMicrobiologyQR1-502ENmSphere, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli ExPEC ST95 antibiotic resistance genomics Microbiology QR1-502
spellingShingle	Escherichia coli ExPEC ST95 antibiotic resistance genomics Microbiology QR1-502 Craig M. Stephens Sheila Adams-Sapper Manraj Sekhon James R. Johnson Lee W. Riley Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
description	ABSTRACT Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other pandemic ExPEC lineages. We took a genomic approach to identify factors that may underlie reduced resistance. We fully assembled genomes for four ST95 isolates representing the four major fimH-based lineages within ST95 and also analyzed draft-level genomes from another 82 ST95 isolates, largely from the western United States. The fully assembled genomes of antibiotic-resistant isolates carried resistance genes exclusively on large (>90-kb) IncFIB/IncFII plasmids. These replicons were common in the draft genomes as well, particularly in antibiotic-resistant isolates, but we also observed multiple instances of a smaller (8.3-kb) ampicillin resistance plasmid that had been previously identified in Salmonella enterica. Among ST95 isolates, pansusceptibility to antibiotics was significantly associated with the fimH6 lineage and the presence of homologs of the previously identified 114-kb IncFIB/IncFII plasmid pUTI89, both of which were also associated with reduced carriage of other plasmids. Potential mechanistic explanations for lineage- and plasmid-specific effects on the prevalence of antibiotic resistance within the ST95 group are discussed. IMPORTANCE Antibiotic resistance in bacterial pathogens is a major public health concern. This work was motivated by the observation that only a small proportion of ST95 isolates, a major pandemic lineage of extraintestinal pathogenic E. coli, have acquired antibiotic resistance, in contrast to many other pandemic lineages. Understanding bacterial genetic factors that may prevent acquisition of resistance could contribute to the development of new biological, medical, or public health strategies to reduce antibiotic-resistant infections.
format	article
author	Craig M. Stephens Sheila Adams-Sapper Manraj Sekhon James R. Johnson Lee W. Riley
author_facet	Craig M. Stephens Sheila Adams-Sapper Manraj Sekhon James R. Johnson Lee W. Riley
author_sort	Craig M. Stephens
title	Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
title_short	Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
title_full	Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
title_fullStr	Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
title_full_unstemmed	Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains
title_sort	genomic analysis of factors associated with low prevalence of antibiotic resistance in extraintestinal pathogenic <named-content content-type="genus-species">escherichia coli</named-content> sequence type 95 strains
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/c9b48a7644a248d68eb79836b1b10fd1
work_keys_str_mv	AT craigmstephens genomicanalysisoffactorsassociatedwithlowprevalenceofantibioticresistanceinextraintestinalpathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentsequencetype95strains AT sheilaadamssapper genomicanalysisoffactorsassociatedwithlowprevalenceofantibioticresistanceinextraintestinalpathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentsequencetype95strains AT manrajsekhon genomicanalysisoffactorsassociatedwithlowprevalenceofantibioticresistanceinextraintestinalpathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentsequencetype95strains AT jamesrjohnson genomicanalysisoffactorsassociatedwithlowprevalenceofantibioticresistanceinextraintestinalpathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentsequencetype95strains AT leewriley genomicanalysisoffactorsassociatedwithlowprevalenceofantibioticresistanceinextraintestinalpathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentsequencetype95strains
_version_	1718428158607228928

Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 95 Strains

Ejemplares similares