Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs

Abstract Most bacteria in nature exist in biofilms, which are inherently tolerant to antibiotics. There is currently very limited understanding of how biofilms evolve in response to sub-lethal concentrations of antimicrobials. In this study, we use a biofilm evolution model to study the effects of s...

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Autores principales:	Eleftheria Trampari, Emma R. Holden, Gregory J. Wickham, Anuradha Ravi, Leonardo de Oliveira Martins, George M. Savva, Mark A. Webber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/c9ba790d305b43acb0b9c148b1734630
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spelling	oai:doaj.org-article:c9ba790d305b43acb0b9c148b17346302021-12-02T15:23:15ZExposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs10.1038/s41522-020-00178-02055-5008https://doaj.org/article/c9ba790d305b43acb0b9c148b17346302021-01-01T00:00:00Zhttps://doi.org/10.1038/s41522-020-00178-0https://doaj.org/toc/2055-5008Abstract Most bacteria in nature exist in biofilms, which are inherently tolerant to antibiotics. There is currently very limited understanding of how biofilms evolve in response to sub-lethal concentrations of antimicrobials. In this study, we use a biofilm evolution model to study the effects of sub-inhibitory concentrations of three antibiotics on Salmonella Typhimurium biofilms. We show that biofilms rapidly evolve resistance to each antibiotic they are exposed to, demonstrating a strong selective pressure on biofilms from low antibiotic concentrations. While all antibiotics tested select for clinical resistance, there is no common mechanism. Adaptation to antimicrobials, however, has a marked cost for other clinically important phenotypes, including biofilm formation and virulence. Cefotaxime selects mutants with the greatest deficit in biofilm formation followed by azithromycin and then ciprofloxacin. Understanding the impacts of exposure of biofilms to antibiotics will help understand evolutionary trajectories and may help guide how best to use antibiotics in a biofilm context. Experimental evolution in combination with whole-genome sequencing is a powerful tool for the prediction of evolution trajectories associated with antibiotic resistance in biofilms.Eleftheria TrampariEmma R. HoldenGregory J. WickhamAnuradha RaviLeonardo de Oliveira MartinsGeorge M. SavvaMark A. WebberNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Eleftheria Trampari Emma R. Holden Gregory J. Wickham Anuradha Ravi Leonardo de Oliveira Martins George M. Savva Mark A. Webber Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
description	Abstract Most bacteria in nature exist in biofilms, which are inherently tolerant to antibiotics. There is currently very limited understanding of how biofilms evolve in response to sub-lethal concentrations of antimicrobials. In this study, we use a biofilm evolution model to study the effects of sub-inhibitory concentrations of three antibiotics on Salmonella Typhimurium biofilms. We show that biofilms rapidly evolve resistance to each antibiotic they are exposed to, demonstrating a strong selective pressure on biofilms from low antibiotic concentrations. While all antibiotics tested select for clinical resistance, there is no common mechanism. Adaptation to antimicrobials, however, has a marked cost for other clinically important phenotypes, including biofilm formation and virulence. Cefotaxime selects mutants with the greatest deficit in biofilm formation followed by azithromycin and then ciprofloxacin. Understanding the impacts of exposure of biofilms to antibiotics will help understand evolutionary trajectories and may help guide how best to use antibiotics in a biofilm context. Experimental evolution in combination with whole-genome sequencing is a powerful tool for the prediction of evolution trajectories associated with antibiotic resistance in biofilms.
format	article
author	Eleftheria Trampari Emma R. Holden Gregory J. Wickham Anuradha Ravi Leonardo de Oliveira Martins George M. Savva Mark A. Webber
author_facet	Eleftheria Trampari Emma R. Holden Gregory J. Wickham Anuradha Ravi Leonardo de Oliveira Martins George M. Savva Mark A. Webber
author_sort	Eleftheria Trampari
title	Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
title_short	Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
title_full	Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
title_fullStr	Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
title_full_unstemmed	Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
title_sort	exposure of salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c9ba790d305b43acb0b9c148b1734630
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Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs

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