Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions

Necrotizing soft tissue infections (NSTI) progress to severe necrosis and result in fatal sepsis within a short time. Vibrio vulnificus is a causative agent and can spread from the initial infection site through soft tissue finally to the systemic circulation of the host. The motility and chemotaxis...

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Autores principales:	Kohei Yamazaki, Takashige Kashimoto, Takehiro Kado, Yukihiro Akeda, Kazuki Yoshioka, Toshio Kodama, Mai Yamamoto, Masashi Okamura, Tsutomu Kakuda, Shunji Ueno
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	vibrio vulnificus necrotizing soft tissue infection pathogenic factor chemotaxis mouse model in vivo imaging Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/c9bbd95c7c684f50907d2ffa4618575f
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spelling	oai:doaj.org-article:c9bbd95c7c684f50907d2ffa4618575f2021-11-17T14:21:58ZChemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions2150-55942150-560810.1080/21505594.2020.1782707https://doaj.org/article/c9bbd95c7c684f50907d2ffa4618575f2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1782707https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Necrotizing soft tissue infections (NSTI) progress to severe necrosis and result in fatal sepsis within a short time. Vibrio vulnificus is a causative agent and can spread from the initial infection site through soft tissue finally to the systemic circulation of the host. The motility and chemotaxis of this bacterium are essential for proliferation and lethality in a murine model of the infection, but their role in pathogenicity has not been characterized. In this study, we revealed the roles of motility and chemotaxis during the process of V. vulnificus infection. We compared a nonmotile mutant and two nonchemotactic mutants with their parent strain (WT) with regard to bacterial spread using an in vivo imaging system (IVIS) and invasion by detection of bacteria from the muscle and spleen of a murine infection model. WT rapidly spread throughout the infected thigh and invaded deep muscle causing severe tissue damage. The detection rate in the systemic circulation and the lethality were high. On the other hand, the nonmotile mutant stayed at the inoculation site, and the nonchemotactic mutants spread only slowly through the soft tissue of the infected thigh. Detection in the systemic circulation, the degree of tissue damage, and the lethality of nonchemotactic mutants were significantly reduced in mice compared with WT. This study demonstrated that chemotaxis is essential for invasion from the infection site to the deep and distant tissues and the main pathogenic factor for the rapid progression leading to sepsis in V. vulnificus NSTI.Kohei YamazakiTakashige KashimotoTakehiro KadoYukihiro AkedaKazuki YoshiokaToshio KodamaMai YamamotoMasashi OkamuraTsutomu KakudaShunji UenoTaylor & Francis Grouparticlevibrio vulnificusnecrotizing soft tissue infectionpathogenic factorchemotaxismouse modelin vivo imagingInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 839-847 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	vibrio vulnificus necrotizing soft tissue infection pathogenic factor chemotaxis mouse model in vivo imaging Infectious and parasitic diseases RC109-216
spellingShingle	vibrio vulnificus necrotizing soft tissue infection pathogenic factor chemotaxis mouse model in vivo imaging Infectious and parasitic diseases RC109-216 Kohei Yamazaki Takashige Kashimoto Takehiro Kado Yukihiro Akeda Kazuki Yoshioka Toshio Kodama Mai Yamamoto Masashi Okamura Tsutomu Kakuda Shunji Ueno Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
description	Necrotizing soft tissue infections (NSTI) progress to severe necrosis and result in fatal sepsis within a short time. Vibrio vulnificus is a causative agent and can spread from the initial infection site through soft tissue finally to the systemic circulation of the host. The motility and chemotaxis of this bacterium are essential for proliferation and lethality in a murine model of the infection, but their role in pathogenicity has not been characterized. In this study, we revealed the roles of motility and chemotaxis during the process of V. vulnificus infection. We compared a nonmotile mutant and two nonchemotactic mutants with their parent strain (WT) with regard to bacterial spread using an in vivo imaging system (IVIS) and invasion by detection of bacteria from the muscle and spleen of a murine infection model. WT rapidly spread throughout the infected thigh and invaded deep muscle causing severe tissue damage. The detection rate in the systemic circulation and the lethality were high. On the other hand, the nonmotile mutant stayed at the inoculation site, and the nonchemotactic mutants spread only slowly through the soft tissue of the infected thigh. Detection in the systemic circulation, the degree of tissue damage, and the lethality of nonchemotactic mutants were significantly reduced in mice compared with WT. This study demonstrated that chemotaxis is essential for invasion from the infection site to the deep and distant tissues and the main pathogenic factor for the rapid progression leading to sepsis in V. vulnificus NSTI.
format	article
author	Kohei Yamazaki Takashige Kashimoto Takehiro Kado Yukihiro Akeda Kazuki Yoshioka Toshio Kodama Mai Yamamoto Masashi Okamura Tsutomu Kakuda Shunji Ueno
author_facet	Kohei Yamazaki Takashige Kashimoto Takehiro Kado Yukihiro Akeda Kazuki Yoshioka Toshio Kodama Mai Yamamoto Masashi Okamura Tsutomu Kakuda Shunji Ueno
author_sort	Kohei Yamazaki
title	Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
title_short	Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
title_full	Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
title_fullStr	Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
title_full_unstemmed	Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions
title_sort	chemotactic invasion in deep soft tissue by vibrio vulnificus is essential for the progression of necrotic lesions
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/c9bbd95c7c684f50907d2ffa4618575f
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Chemotactic invasion in deep soft tissue by Vibrio vulnificus is essential for the progression of necrotic lesions

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