Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.

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Bibliographic Details
Main Authors:	Maria J. Sarmento, Michele Oneto, Simone Pelicci, Luca Pesce, Lorenzo Scipioni, Mario Faretta, Laura Furia, Gaetano Ivan Dellino, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro, Luca Lanzanò
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d
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spelling	oai:doaj.org-article:c9c25d83d741467483f9fddb84e0dc3d2021-12-02T16:49:53ZExploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures10.1038/s41467-018-05963-22041-1723https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05963-2https://doaj.org/toc/2041-1723A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.Maria J. SarmentoMichele OnetoSimone PelicciLuca PesceLorenzo ScipioniMario FarettaLaura FuriaGaetano Ivan DellinoPier Giuseppe PelicciPaolo BianchiniAlberto DiasproLuca LanzanòNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maria J. Sarmento Michele Oneto Simone Pelicci Luca Pesce Lorenzo Scipioni Mario Faretta Laura Furia Gaetano Ivan Dellino Pier Giuseppe Pelicci Paolo Bianchini Alberto Diaspro Luca Lanzanò Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
description	A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.
format	article
author	Maria J. Sarmento Michele Oneto Simone Pelicci Luca Pesce Lorenzo Scipioni Mario Faretta Laura Furia Gaetano Ivan Dellino Pier Giuseppe Pelicci Paolo Bianchini Alberto Diaspro Luca Lanzanò
author_facet	Maria J. Sarmento Michele Oneto Simone Pelicci Luca Pesce Lorenzo Scipioni Mario Faretta Laura Furia Gaetano Ivan Dellino Pier Giuseppe Pelicci Paolo Bianchini Alberto Diaspro Luca Lanzanò
author_sort	Maria J. Sarmento
title	Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_short	Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_full	Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_fullStr	Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_full_unstemmed	Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_sort	exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d
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Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

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