Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.

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Autores principales: Maria J. Sarmento, Michele Oneto, Simone Pelicci, Luca Pesce, Lorenzo Scipioni, Mario Faretta, Laura Furia, Gaetano Ivan Dellino, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro, Luca Lanzanò
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Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d
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spelling oai:doaj.org-article:c9c25d83d741467483f9fddb84e0dc3d2021-12-02T16:49:53ZExploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures10.1038/s41467-018-05963-22041-1723https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05963-2https://doaj.org/toc/2041-1723A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.Maria J. SarmentoMichele OnetoSimone PelicciLuca PesceLorenzo ScipioniMario FarettaLaura FuriaGaetano Ivan DellinoPier Giuseppe PelicciPaolo BianchiniAlberto DiasproLuca LanzanòNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Maria J. Sarmento
Michele Oneto
Simone Pelicci
Luca Pesce
Lorenzo Scipioni
Mario Faretta
Laura Furia
Gaetano Ivan Dellino
Pier Giuseppe Pelicci
Paolo Bianchini
Alberto Diaspro
Luca Lanzanò
Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
description A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.
format article
author Maria J. Sarmento
Michele Oneto
Simone Pelicci
Luca Pesce
Lorenzo Scipioni
Mario Faretta
Laura Furia
Gaetano Ivan Dellino
Pier Giuseppe Pelicci
Paolo Bianchini
Alberto Diaspro
Luca Lanzanò
author_facet Maria J. Sarmento
Michele Oneto
Simone Pelicci
Luca Pesce
Lorenzo Scipioni
Mario Faretta
Laura Furia
Gaetano Ivan Dellino
Pier Giuseppe Pelicci
Paolo Bianchini
Alberto Diaspro
Luca Lanzanò
author_sort Maria J. Sarmento
title Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_short Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_full Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_fullStr Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_full_unstemmed Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
title_sort exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d
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