Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
A known limitation of super-resolution STED microscopy is the need of high laser power which can cause photobleaching and phototoxicity. Here the authors further optimize this method and show that modulating STED intensity during acquisition results in an enhanced resolution and reduced background.
Enregistré dans:
Auteurs principaux: | Maria J. Sarmento, Michele Oneto, Simone Pelicci, Luca Pesce, Lorenzo Scipioni, Mario Faretta, Laura Furia, Gaetano Ivan Dellino, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro, Luca Lanzanò |
---|---|
Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2018
|
Sujets: | |
Accès en ligne: | https://doaj.org/article/c9c25d83d741467483f9fddb84e0dc3d |
Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
Documents similaires
-
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector
par: L. Scipioni, et autres
Publié: (2018) -
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
par: Luca Lanzanò, et autres
Publié: (2017) -
Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
par: Adrien Mau, et autres
Publié: (2021) -
Tunable light and drug induced depletion of target proteins
par: Wen Deng, et autres
Publié: (2020) -
Quantitative Super-Resolution Microscopy to Assess Adhesion of Neuronal Cells on Single-Layer Graphene Substrates
par: Silvia Scalisi, et autres
Publié: (2021)