Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.

The highly conserved ATP-dependent Lon protease plays important roles in diverse biological processes. The lon gene is usually nonessential for viability; however, lon mutants of several bacterial species, although viable, exhibit cellular defects. Here, we show that a lack of Lon protease causes pl...

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Autores principales:	Eunhye Goo, Ingyu Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9c3489e3d874b4ba26c2aa3cd656c1b
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spelling	oai:doaj.org-article:c9c3489e3d874b4ba26c2aa3cd656c1b2021-12-02T20:06:16ZEssential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.1932-620310.1371/journal.pone.0257257https://doaj.org/article/c9c3489e3d874b4ba26c2aa3cd656c1b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257257https://doaj.org/toc/1932-6203The highly conserved ATP-dependent Lon protease plays important roles in diverse biological processes. The lon gene is usually nonessential for viability; however, lon mutants of several bacterial species, although viable, exhibit cellular defects. Here, we show that a lack of Lon protease causes pleiotropic effects in the rice pathogen Burkholderia glumae. The null mutation of lon produced three colony types, big (BLONB), normal (BLONN), and small (BLONS), in Luria-Bertani (LB) medium. Colonies of the BLONB and BLONN types were re-segregated upon subculture, while those of the BLONS type were too small to manipulate. The BLONN type was chosen for further studies, as only this type was fully genetically complemented. BLONN-type cells did not reach the maximum growth capacity, and their population decreased drastically after the stationary phase in LB medium. BLONN-type cells were defective in the biosynthesis of quorum sensing (QS) signals and exhibited reduced oxalate biosynthetic activity, causing environmental alkaline toxicity and population collapse. Addition of excessive N-octanoyl-homoserine lactone (C8-HSL) to BLONN-type cell cultures did not fully restore oxalate biosynthesis, suggesting that the decrease in oxalate biosynthesis in BLONN-type cells was not due to insufficient C8-HSL. Co-expression of lon and tofR in Escherichia coli suggested that Lon negatively affects the TofR level in a C8-HSL-dependent manner. Lon protease interacted with the oxalate biosynthetic enzymes, ObcA and ObcB, indicating potential roles for the oxalate biosynthetic activity. These results suggest that Lon protease influences colony morphology, growth, QS system, and oxalate biosynthesis in B. glumae.Eunhye GooIngyu HwangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eunhye Goo Ingyu Hwang Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
description	The highly conserved ATP-dependent Lon protease plays important roles in diverse biological processes. The lon gene is usually nonessential for viability; however, lon mutants of several bacterial species, although viable, exhibit cellular defects. Here, we show that a lack of Lon protease causes pleiotropic effects in the rice pathogen Burkholderia glumae. The null mutation of lon produced three colony types, big (BLONB), normal (BLONN), and small (BLONS), in Luria-Bertani (LB) medium. Colonies of the BLONB and BLONN types were re-segregated upon subculture, while those of the BLONS type were too small to manipulate. The BLONN type was chosen for further studies, as only this type was fully genetically complemented. BLONN-type cells did not reach the maximum growth capacity, and their population decreased drastically after the stationary phase in LB medium. BLONN-type cells were defective in the biosynthesis of quorum sensing (QS) signals and exhibited reduced oxalate biosynthetic activity, causing environmental alkaline toxicity and population collapse. Addition of excessive N-octanoyl-homoserine lactone (C8-HSL) to BLONN-type cell cultures did not fully restore oxalate biosynthesis, suggesting that the decrease in oxalate biosynthesis in BLONN-type cells was not due to insufficient C8-HSL. Co-expression of lon and tofR in Escherichia coli suggested that Lon negatively affects the TofR level in a C8-HSL-dependent manner. Lon protease interacted with the oxalate biosynthetic enzymes, ObcA and ObcB, indicating potential roles for the oxalate biosynthetic activity. These results suggest that Lon protease influences colony morphology, growth, QS system, and oxalate biosynthesis in B. glumae.
format	article
author	Eunhye Goo Ingyu Hwang
author_facet	Eunhye Goo Ingyu Hwang
author_sort	Eunhye Goo
title	Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
title_short	Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
title_full	Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
title_fullStr	Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
title_full_unstemmed	Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.
title_sort	essential roles of lon protease in the morpho-physiological traits of the rice pathogen burkholderia glumae.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c9c3489e3d874b4ba26c2aa3cd656c1b
work_keys_str_mv	AT eunhyegoo essentialrolesoflonproteaseinthemorphophysiologicaltraitsofthericepathogenburkholderiaglumae AT ingyuhwang essentialrolesoflonproteaseinthemorphophysiologicaltraitsofthericepathogenburkholderiaglumae
_version_	1718375449294274560

Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae.

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