JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis

Agrobacterium tumefaciens can cause crown gall tumors by transferring both an oncogenic piece of DNA (T-DNA) and several effector proteins into a wide range of host plants. For the translocated effector VirE3 multiple functions have been reported. It acts as a transcription factor in the nucleus bin...

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Autores principales:	Shijuan Li, Bingliang Xu, Xiaolei Niu, Xiang Lu, Jianping Cheng, Meiliang Zhou, Paul J. J. Hooykaas
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Agrobacterium tumefaciens Arabidopsis thaliana VirE3 JAZ8 jasmonates Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/c9c5c2e6ac5e4703a04a9b028a987cf2
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spelling	oai:doaj.org-article:c9c5c2e6ac5e4703a04a9b028a987cf22021-11-19T04:50:12ZJAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis1664-462X10.3389/fpls.2021.685533https://doaj.org/article/c9c5c2e6ac5e4703a04a9b028a987cf22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.685533/fullhttps://doaj.org/toc/1664-462XAgrobacterium tumefaciens can cause crown gall tumors by transferring both an oncogenic piece of DNA (T-DNA) and several effector proteins into a wide range of host plants. For the translocated effector VirE3 multiple functions have been reported. It acts as a transcription factor in the nucleus binding to the Arabidopsis thaliana pBrp TFIIB-like protein to activate the expression of VBF, an F-box protein involved in degradation of the VirE2 and VIP1 proteins, facilitating Agrobacterium-mediated transformation. Also VirE3 has been found at the plasma membrane, where it could interact with VirE2. Here, we identified AtJAZ8 in a yeast two-hybrid screening with VirE3 as a bait and confirmed the interaction by pull-down and bimolecular fluorescence complementation assays. We also found that the deletion of virE3 reduced Agrobacterium virulence in a root tumor assay. Overexpression of virE3 in Arabidopsis enhanced tumorigenesis, whereas overexpression of AtJAZ8 in Arabidopsis significantly decreased the numbers of tumors formed. Further experiments demonstrated that AtJAZ8 inhibited the activity of VirE3 as a plant transcriptional regulator, and overexpression of AtJAZ8 in Arabidopsis activated AtPR1 gene expression while it repressed the expression of AtPDF1.2. Conversely, overexpression of virE3 in Arabidopsis suppressed the expression of AtPR1 whereas activated the expression of AtPDF1.2. Our results proposed a novel mechanism of counter defense signaling pathways used by Agrobacterium, suggesting that VirE3 and JAZ8 may antagonistically modulate the salicylic acid/jasmonic acid (SA/JA)-mediated plant defense signaling response during Agrobacterium infection.Shijuan LiBingliang XuXiaolei NiuXiang LuJianping ChengMeiliang ZhouPaul J. J. HooykaasFrontiers Media S.A.articleAgrobacterium tumefaciensArabidopsis thalianaVirE3JAZ8jasmonatesPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Agrobacterium tumefaciens Arabidopsis thaliana VirE3 JAZ8 jasmonates Plant culture SB1-1110
spellingShingle	Agrobacterium tumefaciens Arabidopsis thaliana VirE3 JAZ8 jasmonates Plant culture SB1-1110 Shijuan Li Bingliang Xu Xiaolei Niu Xiang Lu Jianping Cheng Meiliang Zhou Paul J. J. Hooykaas JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
description	Agrobacterium tumefaciens can cause crown gall tumors by transferring both an oncogenic piece of DNA (T-DNA) and several effector proteins into a wide range of host plants. For the translocated effector VirE3 multiple functions have been reported. It acts as a transcription factor in the nucleus binding to the Arabidopsis thaliana pBrp TFIIB-like protein to activate the expression of VBF, an F-box protein involved in degradation of the VirE2 and VIP1 proteins, facilitating Agrobacterium-mediated transformation. Also VirE3 has been found at the plasma membrane, where it could interact with VirE2. Here, we identified AtJAZ8 in a yeast two-hybrid screening with VirE3 as a bait and confirmed the interaction by pull-down and bimolecular fluorescence complementation assays. We also found that the deletion of virE3 reduced Agrobacterium virulence in a root tumor assay. Overexpression of virE3 in Arabidopsis enhanced tumorigenesis, whereas overexpression of AtJAZ8 in Arabidopsis significantly decreased the numbers of tumors formed. Further experiments demonstrated that AtJAZ8 inhibited the activity of VirE3 as a plant transcriptional regulator, and overexpression of AtJAZ8 in Arabidopsis activated AtPR1 gene expression while it repressed the expression of AtPDF1.2. Conversely, overexpression of virE3 in Arabidopsis suppressed the expression of AtPR1 whereas activated the expression of AtPDF1.2. Our results proposed a novel mechanism of counter defense signaling pathways used by Agrobacterium, suggesting that VirE3 and JAZ8 may antagonistically modulate the salicylic acid/jasmonic acid (SA/JA)-mediated plant defense signaling response during Agrobacterium infection.
format	article
author	Shijuan Li Bingliang Xu Xiaolei Niu Xiang Lu Jianping Cheng Meiliang Zhou Paul J. J. Hooykaas
author_facet	Shijuan Li Bingliang Xu Xiaolei Niu Xiang Lu Jianping Cheng Meiliang Zhou Paul J. J. Hooykaas
author_sort	Shijuan Li
title	JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
title_short	JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
title_full	JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
title_fullStr	JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
title_full_unstemmed	JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
title_sort	jaz8 interacts with vire3 attenuating agrobacterium mediated root tumorigenesis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c9c5c2e6ac5e4703a04a9b028a987cf2
work_keys_str_mv	AT shijuanli jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT bingliangxu jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT xiaoleiniu jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT xianglu jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT jianpingcheng jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT meiliangzhou jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis AT pauljjhooykaas jaz8interactswithvire3attenuatingagrobacteriummediatedroottumorigenesis
_version_	1718420401680285696

JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis

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