Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protoc...

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Autores principales: Haitao Chen, Yan Zeng, Yongzhi Yang, Lingli Huang, Bolin Tang, He Zhang, Fei Hao, Wei Liu, Youhan Li, Yanbin Liu, Xiaoshuang Zhang, Ru Zhang, Yesheng Zhang, Yongxin Li, Kun Wang, Hua He, Zhongkai Wang, Guangyi Fan, Hui Yang, Aike Bao, Zhanhuan Shang, Jianghua Chen, Wen Wang, Qiang Qiu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/c9c662acf2c2427fb705e058ea826493
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Sumario:Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protocol.

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