Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protoc...

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Autores principales:	Haitao Chen, Yan Zeng, Yongzhi Yang, Lingli Huang, Bolin Tang, He Zhang, Fei Hao, Wei Liu, Youhan Li, Yanbin Liu, Xiaoshuang Zhang, Ru Zhang, Yesheng Zhang, Yongxin Li, Kun Wang, Hua He, Zhongkai Wang, Guangyi Fan, Hui Yang, Aike Bao, Zhanhuan Shang, Jianghua Chen, Wen Wang, Qiang Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c9c662acf2c2427fb705e058ea826493
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spelling	oai:doaj.org-article:c9c662acf2c2427fb705e058ea8264932021-12-02T14:58:44ZAllele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa10.1038/s41467-020-16338-x2041-1723https://doaj.org/article/c9c662acf2c2427fb705e058ea8264932020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16338-xhttps://doaj.org/toc/2041-1723Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protocol.Haitao ChenYan ZengYongzhi YangLingli HuangBolin TangHe ZhangFei HaoWei LiuYouhan LiYanbin LiuXiaoshuang ZhangRu ZhangYesheng ZhangYongxin LiKun WangHua HeZhongkai WangGuangyi FanHui YangAike BaoZhanhuan ShangJianghua ChenWen WangQiang QiuNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Haitao Chen Yan Zeng Yongzhi Yang Lingli Huang Bolin Tang He Zhang Fei Hao Wei Liu Youhan Li Yanbin Liu Xiaoshuang Zhang Ru Zhang Yesheng Zhang Yongxin Li Kun Wang Hua He Zhongkai Wang Guangyi Fan Hui Yang Aike Bao Zhanhuan Shang Jianghua Chen Wen Wang Qiang Qiu Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
description	Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protocol.
format	article
author	Haitao Chen Yan Zeng Yongzhi Yang Lingli Huang Bolin Tang He Zhang Fei Hao Wei Liu Youhan Li Yanbin Liu Xiaoshuang Zhang Ru Zhang Yesheng Zhang Yongxin Li Kun Wang Hua He Zhongkai Wang Guangyi Fan Hui Yang Aike Bao Zhanhuan Shang Jianghua Chen Wen Wang Qiang Qiu
author_facet	Haitao Chen Yan Zeng Yongzhi Yang Lingli Huang Bolin Tang He Zhang Fei Hao Wei Liu Youhan Li Yanbin Liu Xiaoshuang Zhang Ru Zhang Yesheng Zhang Yongxin Li Kun Wang Hua He Zhongkai Wang Guangyi Fan Hui Yang Aike Bao Zhanhuan Shang Jianghua Chen Wen Wang Qiang Qiu
author_sort	Haitao Chen
title	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
title_short	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
title_full	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
title_fullStr	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
title_full_unstemmed	Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
title_sort	allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c9c662acf2c2427fb705e058ea826493
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Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

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