codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells

Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections...

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Autores principales:	Vânia Gaio, Nathalie Lopes, Nuno Cerca, Angela França
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Staphylococcus epidermidis infections VBNC cells induction model planktonic cells biofilms glucose magnesium Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c9ce795a504245c7afd14ba9f0439b05
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spelling	oai:doaj.org-article:c9ce795a504245c7afd14ba9f0439b052021-11-15T05:01:47ZcodY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells2235-298810.3389/fcimb.2021.771666https://doaj.org/article/c9ce795a504245c7afd14ba9f0439b052021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.771666/fullhttps://doaj.org/toc/2235-2988Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections. Previous transcriptomics analysis of S. epidermidis strain 9142 biofilms with higher proportions of VBNC cells suggested that the genes pdhA, codY and mazEF could be involved in the induction of the VBNC state. However, it was previously demonstrated that VBNC induction is strain-dependent. To properly assess the role of these genes in VBNC induction, the construction of mutant strains is necessary. Thus, herein, we assessed if VBNC cells could be induced in strain 1457, a strain amenable to genetic manipulation, and if the previously identified genes were involved in the modulation of the VBNC state in this strain. Furthermore, we evaluated the formation of VBNC cells on planktonic cultures. Our results showed that despite being commonly associated with biofilms, the proportion of VBNC cells can be modulated in both biofilm and planktonic cultures and that the expression of codY and pdhA was upregulated under VBNC inducing conditions in both phenotypes. Overall, our study revealed that the formation of VBNC cells in S. epidermidis is independent of the mode of growth and that the genes codY and pdhA seem to be relevant for the regulation of this physiological condition.Vânia GaioNathalie LopesNuno CercaAngela FrançaFrontiers Media S.A.articleStaphylococcus epidermidis infectionsVBNC cells induction modelplanktonic cellsbiofilmsglucosemagnesiumMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus epidermidis infections VBNC cells induction model planktonic cells biofilms glucose magnesium Microbiology QR1-502
spellingShingle	Staphylococcus epidermidis infections VBNC cells induction model planktonic cells biofilms glucose magnesium Microbiology QR1-502 Vânia Gaio Nathalie Lopes Nuno Cerca Angela França codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
description	Staphylococcus epidermidis biofilm cells can enter a physiological state known as viable but non-culturable (VBNC), where, despite being alive, they do not grow in conventional laboratory media. As such, the presence of VBNC cells impacts the diagnosis of S. epidermidis biofilm-associated infections. Previous transcriptomics analysis of S. epidermidis strain 9142 biofilms with higher proportions of VBNC cells suggested that the genes pdhA, codY and mazEF could be involved in the induction of the VBNC state. However, it was previously demonstrated that VBNC induction is strain-dependent. To properly assess the role of these genes in VBNC induction, the construction of mutant strains is necessary. Thus, herein, we assessed if VBNC cells could be induced in strain 1457, a strain amenable to genetic manipulation, and if the previously identified genes were involved in the modulation of the VBNC state in this strain. Furthermore, we evaluated the formation of VBNC cells on planktonic cultures. Our results showed that despite being commonly associated with biofilms, the proportion of VBNC cells can be modulated in both biofilm and planktonic cultures and that the expression of codY and pdhA was upregulated under VBNC inducing conditions in both phenotypes. Overall, our study revealed that the formation of VBNC cells in S. epidermidis is independent of the mode of growth and that the genes codY and pdhA seem to be relevant for the regulation of this physiological condition.
format	article
author	Vânia Gaio Nathalie Lopes Nuno Cerca Angela França
author_facet	Vânia Gaio Nathalie Lopes Nuno Cerca Angela França
author_sort	Vânia Gaio
title	codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_short	codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_full	codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_fullStr	codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_full_unstemmed	codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells
title_sort	cody and pdha expression is induced in staphylococcus epidermidis biofilm and planktonic populations with higher proportions of viable but non-culturable cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c9ce795a504245c7afd14ba9f0439b05
work_keys_str_mv	AT vaniagaio codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells AT nathalielopes codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells AT nunocerca codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells AT angelafranca codyandpdhaexpressionisinducedinstaphylococcusepidermidisbiofilmandplanktonicpopulationswithhigherproportionsofviablebutnonculturablecells
_version_	1718428830789533696

codY and pdhA Expression Is Induced in Staphylococcus epidermidis Biofilm and Planktonic Populations With Higher Proportions of Viable but Non-Culturable Cells

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