Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

Abstract Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being eva...

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Autores principales:	Won Il Choi, Abhishek Sahu, Cristian Vilos, Nazila Kamaly, Seong-Min Jo, Jin Hyung Lee, Giyoong Tae
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9d2f69ccb604a70b0037d2c98b75495
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spelling	oai:doaj.org-article:c9d2f69ccb604a70b0037d2c98b754952021-12-02T15:04:56ZBioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors10.1038/s41598-017-14040-52045-2322https://doaj.org/article/c9d2f69ccb604a70b0037d2c98b754952017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14040-5https://doaj.org/toc/2045-2322Abstract Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.Won Il ChoiAbhishek SahuCristian VilosNazila KamalySeong-Min JoJin Hyung LeeGiyoong TaeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Won Il Choi Abhishek Sahu Cristian Vilos Nazila Kamaly Seong-Min Jo Jin Hyung Lee Giyoong Tae Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
description	Abstract Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.
format	article
author	Won Il Choi Abhishek Sahu Cristian Vilos Nazila Kamaly Seong-Min Jo Jin Hyung Lee Giyoong Tae
author_facet	Won Il Choi Abhishek Sahu Cristian Vilos Nazila Kamaly Seong-Min Jo Jin Hyung Lee Giyoong Tae
author_sort	Won Il Choi
title	Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
title_short	Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
title_full	Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
title_fullStr	Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
title_full_unstemmed	Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors
title_sort	bioinspired heparin nanosponge prepared by photo-crosslinking for controlled release of growth factors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c9d2f69ccb604a70b0037d2c98b75495
work_keys_str_mv	AT wonilchoi bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT abhisheksahu bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT cristianvilos bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT nazilakamaly bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT seongminjo bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT jinhyunglee bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors AT giyoongtae bioinspiredheparinnanospongepreparedbyphotocrosslinkingforcontrolledreleaseofgrowthfactors
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Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

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