Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.

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Detalles Bibliográficos
Autores principales: Yicong Wu, Abhishek Kumar, Corey Smith, Evan Ardiel, Panagiotis Chandris, Ryan Christensen, Ivan Rey-Suarez, Min Guo, Harshad D. Vishwasrao, Jiji Chen, Jianyong Tang, Arpita Upadhyaya, Patrick J. La Riviere, Hari Shroff
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/c9d70825284743998dcbe927064d2824
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Descripción
Sumario:Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.