Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.

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Autores principales: Yicong Wu, Abhishek Kumar, Corey Smith, Evan Ardiel, Panagiotis Chandris, Ryan Christensen, Ivan Rey-Suarez, Min Guo, Harshad D. Vishwasrao, Jiji Chen, Jianyong Tang, Arpita Upadhyaya, Patrick J. La Riviere, Hari Shroff
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/c9d70825284743998dcbe927064d2824
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spelling oai:doaj.org-article:c9d70825284743998dcbe927064d28242021-12-02T17:06:00ZReflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy10.1038/s41467-017-01250-82041-1723https://doaj.org/article/c9d70825284743998dcbe927064d28242017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01250-8https://doaj.org/toc/2041-1723Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.Yicong WuAbhishek KumarCorey SmithEvan ArdielPanagiotis ChandrisRyan ChristensenIvan Rey-SuarezMin GuoHarshad D. VishwasraoJiji ChenJianyong TangArpita UpadhyayaPatrick J. La RiviereHari ShroffNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Yicong Wu
Abhishek Kumar
Corey Smith
Evan Ardiel
Panagiotis Chandris
Ryan Christensen
Ivan Rey-Suarez
Min Guo
Harshad D. Vishwasrao
Jiji Chen
Jianyong Tang
Arpita Upadhyaya
Patrick J. La Riviere
Hari Shroff
Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
description Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.
format article
author Yicong Wu
Abhishek Kumar
Corey Smith
Evan Ardiel
Panagiotis Chandris
Ryan Christensen
Ivan Rey-Suarez
Min Guo
Harshad D. Vishwasrao
Jiji Chen
Jianyong Tang
Arpita Upadhyaya
Patrick J. La Riviere
Hari Shroff
author_facet Yicong Wu
Abhishek Kumar
Corey Smith
Evan Ardiel
Panagiotis Chandris
Ryan Christensen
Ivan Rey-Suarez
Min Guo
Harshad D. Vishwasrao
Jiji Chen
Jianyong Tang
Arpita Upadhyaya
Patrick J. La Riviere
Hari Shroff
author_sort Yicong Wu
title Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_short Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_full Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_fullStr Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_full_unstemmed Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_sort reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/c9d70825284743998dcbe927064d2824
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AT evanardiel reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
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AT harshaddvishwasrao reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
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AT jianyongtang reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
AT arpitaupadhyaya reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
AT patrickjlariviere reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
AT harishroff reflectiveimagingimprovesspatiotemporalresolutionandcollectionefficiencyinlightsheetmicroscopy
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