Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells

Abstract The Cre/loxP system is a powerful tool for generating conditional gene knockout (KO) mice and elucidate gene function in vivo. CD19-Cre and Mb1-iCre transgenic mice are commonly used for generating B cell-specific KO mice and investigate the development, as well as the physiological and pat...

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Autores principales:	Tomoharu Yasuda, Yuichi Saito, Chisato Ono, Kazuhiko Kawata, Akemi Baba, Yoshihiro Baba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9d7f962546b4556b38d6cddb656c148
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spelling	oai:doaj.org-article:c9d7f962546b4556b38d6cddb656c1482021-12-02T11:35:41ZGeneration and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells10.1038/s41598-021-84786-62045-2322https://doaj.org/article/c9d7f962546b4556b38d6cddb656c1482021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84786-6https://doaj.org/toc/2045-2322Abstract The Cre/loxP system is a powerful tool for generating conditional gene knockout (KO) mice and elucidate gene function in vivo. CD19-Cre and Mb1-iCre transgenic mice are commonly used for generating B cell-specific KO mice and investigate the development, as well as the physiological and pathophysiological roles of B cells. However, the CD19-Cre line low efficiency and the Mb1-iCre line occasional ectopic recombination represent challenges for their use. Thus, we developed a CD19-codon-improved Cre (CD19-iCre) knock-in mouse with the T2A-iCre sequence inserted into the Cd19 locus, just before the stop codon. The CD19-iCre mice were compared with existing models, crossed with the Rosa26-EYFP reporter mice, and their recombination activity in B cells carrying different Cre alleles was assessed. CD19-iCre mice showed more effective Cre recombination in the early B cell developmental stages compared with the CD19-Cre mice. The efficiencies of the CD19-iCre and Mb1-iCre lines were similar; however, the B lineage-specific recombination was more stringent in the CD19-iCre line. Furthermore, the utility value of the CD19-iCre model was superior than that of the CD19-Cre mice regarding deletion efficiency in IL10-floxed mice. Thus, the CD19-iCre line is a valuable tool for highly efficient gene targeting specific to the B cell compartment.Tomoharu YasudaYuichi SaitoChisato OnoKazuhiko KawataAkemi BabaYoshihiro BabaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoharu Yasuda Yuichi Saito Chisato Ono Kazuhiko Kawata Akemi Baba Yoshihiro Baba Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
description	Abstract The Cre/loxP system is a powerful tool for generating conditional gene knockout (KO) mice and elucidate gene function in vivo. CD19-Cre and Mb1-iCre transgenic mice are commonly used for generating B cell-specific KO mice and investigate the development, as well as the physiological and pathophysiological roles of B cells. However, the CD19-Cre line low efficiency and the Mb1-iCre line occasional ectopic recombination represent challenges for their use. Thus, we developed a CD19-codon-improved Cre (CD19-iCre) knock-in mouse with the T2A-iCre sequence inserted into the Cd19 locus, just before the stop codon. The CD19-iCre mice were compared with existing models, crossed with the Rosa26-EYFP reporter mice, and their recombination activity in B cells carrying different Cre alleles was assessed. CD19-iCre mice showed more effective Cre recombination in the early B cell developmental stages compared with the CD19-Cre mice. The efficiencies of the CD19-iCre and Mb1-iCre lines were similar; however, the B lineage-specific recombination was more stringent in the CD19-iCre line. Furthermore, the utility value of the CD19-iCre model was superior than that of the CD19-Cre mice regarding deletion efficiency in IL10-floxed mice. Thus, the CD19-iCre line is a valuable tool for highly efficient gene targeting specific to the B cell compartment.
format	article
author	Tomoharu Yasuda Yuichi Saito Chisato Ono Kazuhiko Kawata Akemi Baba Yoshihiro Baba
author_facet	Tomoharu Yasuda Yuichi Saito Chisato Ono Kazuhiko Kawata Akemi Baba Yoshihiro Baba
author_sort	Tomoharu Yasuda
title	Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
title_short	Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
title_full	Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
title_fullStr	Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
title_full_unstemmed	Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells
title_sort	generation and characterization of cd19-icre mice as a tool for efficient and specific conditional gene targeting in b cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c9d7f962546b4556b38d6cddb656c148
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Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells

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