The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface

Abstract Identification of novel therapeutic targets is required for developing alternate strategies to treat infections caused by the extensively drug-resistant bacterial pathogen, Acinetobacter baumannii. As capsular polysaccharide (CPS) is a prime virulence determinant required for evasion of hos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jacob Tickner, Sophia Hawas, Makrina Totsika, Johanna J. Kenyon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9d8f136e9514067a256e125f8716acc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c9d8f136e9514067a256e125f8716acc
record_format	dspace
spelling	oai:doaj.org-article:c9d8f136e9514067a256e125f8716acc2021-11-08T10:46:52ZThe Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface10.1038/s41598-021-01206-52045-2322https://doaj.org/article/c9d8f136e9514067a256e125f8716acc2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01206-5https://doaj.org/toc/2045-2322Abstract Identification of novel therapeutic targets is required for developing alternate strategies to treat infections caused by the extensively drug-resistant bacterial pathogen, Acinetobacter baumannii. As capsular polysaccharide (CPS) is a prime virulence determinant required for evasion of host immune defenses, understanding the pathways for synthesis and assembly of this discrete cell-surface barrier is important. In this study, we assess cell-bound and cell-free CPS material from A. baumannii AB5075 wildtype and transposon library mutants and demonstrate that the Wzi outer membrane protein is required for the proper assembly of the CPS layer on the cell surface. Loss of Wzi resulted in an estimated 4.4-fold reduction in cell-associated CPS with a reciprocal increase in CPS material shed in the extracellular surrounds. Transmission electron microscopy revealed a disrupted CPS layer with sparse patches of CPS on the external face of the outer membrane when Wzi function was lost. However, this genotype did not have a significant effect on biofilm formation. Genetic analysis demonstrated that the wzi gene is ubiquitous in the species, though the nucleotide sequences were surprisingly diverse. Though divergence was not concomitant with variation at the CPS biosynthesis K locus, an association between wzi type and the first sugar of the CPS representing the base of the structure most likely to interact with Wzi was observed.Jacob TicknerSophia HawasMakrina TotsikaJohanna J. KenyonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacob Tickner Sophia Hawas Makrina Totsika Johanna J. Kenyon The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
description	Abstract Identification of novel therapeutic targets is required for developing alternate strategies to treat infections caused by the extensively drug-resistant bacterial pathogen, Acinetobacter baumannii. As capsular polysaccharide (CPS) is a prime virulence determinant required for evasion of host immune defenses, understanding the pathways for synthesis and assembly of this discrete cell-surface barrier is important. In this study, we assess cell-bound and cell-free CPS material from A. baumannii AB5075 wildtype and transposon library mutants and demonstrate that the Wzi outer membrane protein is required for the proper assembly of the CPS layer on the cell surface. Loss of Wzi resulted in an estimated 4.4-fold reduction in cell-associated CPS with a reciprocal increase in CPS material shed in the extracellular surrounds. Transmission electron microscopy revealed a disrupted CPS layer with sparse patches of CPS on the external face of the outer membrane when Wzi function was lost. However, this genotype did not have a significant effect on biofilm formation. Genetic analysis demonstrated that the wzi gene is ubiquitous in the species, though the nucleotide sequences were surprisingly diverse. Though divergence was not concomitant with variation at the CPS biosynthesis K locus, an association between wzi type and the first sugar of the CPS representing the base of the structure most likely to interact with Wzi was observed.
format	article
author	Jacob Tickner Sophia Hawas Makrina Totsika Johanna J. Kenyon
author_facet	Jacob Tickner Sophia Hawas Makrina Totsika Johanna J. Kenyon
author_sort	Jacob Tickner
title	The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
title_short	The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
title_full	The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
title_fullStr	The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
title_full_unstemmed	The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
title_sort	wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the acinetobacter baumannii cell surface
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c9d8f136e9514067a256e125f8716acc
work_keys_str_mv	AT jacobtickner thewzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT sophiahawas thewzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT makrinatotsika thewzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT johannajkenyon thewzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT jacobtickner wzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT sophiahawas wzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT makrinatotsika wzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface AT johannajkenyon wzioutermembraneproteinmediatesassemblyofatightcapsularpolysaccharidelayerontheacinetobacterbaumanniicellsurface
_version_	1718442660323131392

The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface

Ejemplares similares