Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats

Abstract Sepsis causes multiple organs failures and eventually death. Changes in blood constituents due to sepsis lead to alterations in hemorheological properties, and cell adhesiveness. In this study, a new microfluidic system is proposed to measure temporal variations in biophysical properties of...

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Autores principales:	Eunseop Yeom, Hye Mi Kim, Jun Hong Park, Woorak Choi, Junsang Doh, Sang Joon Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9dd265acec04bbe87094de954ec8e33
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spelling	oai:doaj.org-article:c9dd265acec04bbe87094de954ec8e332021-12-02T12:30:12ZMicrofluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats10.1038/s41598-017-01985-w2045-2322https://doaj.org/article/c9dd265acec04bbe87094de954ec8e332017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01985-whttps://doaj.org/toc/2045-2322Abstract Sepsis causes multiple organs failures and eventually death. Changes in blood constituents due to sepsis lead to alterations in hemorheological properties, and cell adhesiveness. In this study, a new microfluidic system is proposed to measure temporal variations in biophysical properties of blood after injecting lipopolysaccharide (LPS) into a rat extracorporeal model under ex vivo condition. To measure blood viscosity, the interfacial line between blood and a reference fluid is formed in a Y-shaped channel. Based on the relation between interfacial width and pressure ratio, the temporal variation in blood viscosity is estimated. Optical images of blood flows are analyzed by decreasing flow rate for examination of red blood cell (RBC) aggregation. Platelets initiated by shear acceleration around the stenosis adhere to the post-stenosed region. By applying a correlation map that visualizes the decorrelation of the streaming blood flow, the area of adhered platelets can be quantitatively attained without labeling of platelets. To assess sepsis inflammation, conventional biomarkers (PCT and IL-8) are also monitored. The increasing tendency for blood viscosity, RBC aggregation, platelet adhesion, and septic biomarkers are observed after LPS injection. This microfluidic system would be beneficial for monitoring the changes in hemorheological properties and platelet activation caused by sepsis.Eunseop YeomHye Mi KimJun Hong ParkWoorak ChoiJunsang DohSang Joon LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eunseop Yeom Hye Mi Kim Jun Hong Park Woorak Choi Junsang Doh Sang Joon Lee Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
description	Abstract Sepsis causes multiple organs failures and eventually death. Changes in blood constituents due to sepsis lead to alterations in hemorheological properties, and cell adhesiveness. In this study, a new microfluidic system is proposed to measure temporal variations in biophysical properties of blood after injecting lipopolysaccharide (LPS) into a rat extracorporeal model under ex vivo condition. To measure blood viscosity, the interfacial line between blood and a reference fluid is formed in a Y-shaped channel. Based on the relation between interfacial width and pressure ratio, the temporal variation in blood viscosity is estimated. Optical images of blood flows are analyzed by decreasing flow rate for examination of red blood cell (RBC) aggregation. Platelets initiated by shear acceleration around the stenosis adhere to the post-stenosed region. By applying a correlation map that visualizes the decorrelation of the streaming blood flow, the area of adhered platelets can be quantitatively attained without labeling of platelets. To assess sepsis inflammation, conventional biomarkers (PCT and IL-8) are also monitored. The increasing tendency for blood viscosity, RBC aggregation, platelet adhesion, and septic biomarkers are observed after LPS injection. This microfluidic system would be beneficial for monitoring the changes in hemorheological properties and platelet activation caused by sepsis.
format	article
author	Eunseop Yeom Hye Mi Kim Jun Hong Park Woorak Choi Junsang Doh Sang Joon Lee
author_facet	Eunseop Yeom Hye Mi Kim Jun Hong Park Woorak Choi Junsang Doh Sang Joon Lee
author_sort	Eunseop Yeom
title	Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
title_short	Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
title_full	Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
title_fullStr	Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
title_full_unstemmed	Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats
title_sort	microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in lps-injected rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c9dd265acec04bbe87094de954ec8e33
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Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats

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