Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model

Abstract Control of blood clotting in root canal systems is one of the most critical and difficult concerns for regenerative endodontics therapy (RET). The purpose of this study was to investigate the effects of using gelatin- and fibrin-based hemostatic hydrogels as a scaffold on pulp regeneration...

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Autores principales:	Ji-Hyun Jang, Joung-Ho Moon, Sahng Gyoon Kim, Sun-Young Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9e25117a526463fb3e09402b8389f39
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spelling	oai:doaj.org-article:c9e25117a526463fb3e09402b8389f392021-12-02T16:06:39ZPulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model10.1038/s41598-020-69437-62045-2322https://doaj.org/article/c9e25117a526463fb3e09402b8389f392020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69437-6https://doaj.org/toc/2045-2322Abstract Control of blood clotting in root canal systems is one of the most critical and difficult concerns for regenerative endodontics therapy (RET). The purpose of this study was to investigate the effects of using gelatin- and fibrin-based hemostatic hydrogels as a scaffold on pulp regeneration in a minipig model. Cell viability of human dental pulp stem cells cultured three-dimensionally in gelatin-based and fibrin-based scaffolds was evaluated by MTT and live/dead assay. RET was performed on 24 immature premolars with an autologous blood clot (PC), gelatin-based and fibrin-based hemostatic matrices (GM and FM), or without the insertion of a scaffold (NC). The follow-up period was 12 weeks. Radiographic and histologic assessments for pulp regeneration were performed. Gelatin-based scaffolds exhibited significantly higher cell viability than fibrin-based scaffolds after 15 days (P < 0.05). The PC and GM groups showed favorable root development without inflammation and newly mineralized tissue deposited in the root canal system, while FM group presented inflammatory changes with the continuation of root development. The NC group exhibited internal root resorption with periapical lesions. The application of GM in RET led to favorable clinical outcomes of root development without inflammatory changes compared to conventional RET. Our results suggest that GM may serve as a viable regenerative scaffold for pulp regeneration.Ji-Hyun JangJoung-Ho MoonSahng Gyoon KimSun-Young KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-Hyun Jang Joung-Ho Moon Sahng Gyoon Kim Sun-Young Kim Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
description	Abstract Control of blood clotting in root canal systems is one of the most critical and difficult concerns for regenerative endodontics therapy (RET). The purpose of this study was to investigate the effects of using gelatin- and fibrin-based hemostatic hydrogels as a scaffold on pulp regeneration in a minipig model. Cell viability of human dental pulp stem cells cultured three-dimensionally in gelatin-based and fibrin-based scaffolds was evaluated by MTT and live/dead assay. RET was performed on 24 immature premolars with an autologous blood clot (PC), gelatin-based and fibrin-based hemostatic matrices (GM and FM), or without the insertion of a scaffold (NC). The follow-up period was 12 weeks. Radiographic and histologic assessments for pulp regeneration were performed. Gelatin-based scaffolds exhibited significantly higher cell viability than fibrin-based scaffolds after 15 days (P < 0.05). The PC and GM groups showed favorable root development without inflammation and newly mineralized tissue deposited in the root canal system, while FM group presented inflammatory changes with the continuation of root development. The NC group exhibited internal root resorption with periapical lesions. The application of GM in RET led to favorable clinical outcomes of root development without inflammatory changes compared to conventional RET. Our results suggest that GM may serve as a viable regenerative scaffold for pulp regeneration.
format	article
author	Ji-Hyun Jang Joung-Ho Moon Sahng Gyoon Kim Sun-Young Kim
author_facet	Ji-Hyun Jang Joung-Ho Moon Sahng Gyoon Kim Sun-Young Kim
author_sort	Ji-Hyun Jang
title	Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
title_short	Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
title_full	Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
title_fullStr	Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
title_full_unstemmed	Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
title_sort	pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c9e25117a526463fb3e09402b8389f39
work_keys_str_mv	AT jihyunjang pulpregenerationwithhemostaticmatricesasascaffoldinanimmaturetoothminipigmodel AT jounghomoon pulpregenerationwithhemostaticmatricesasascaffoldinanimmaturetoothminipigmodel AT sahnggyoonkim pulpregenerationwithhemostaticmatricesasascaffoldinanimmaturetoothminipigmodel AT sunyoungkim pulpregenerationwithhemostaticmatricesasascaffoldinanimmaturetoothminipigmodel
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Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model

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