Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma

Fluoro-edenite (FE) is an asbestiform fiber identified in Biancavilla (Sicily, Italy). Environmental exposure to FE has been associated with a higher incidence of malignant mesothelioma (MM). The present study aimed to validate the predicted diagnostic significance of hsa-miR-323a-3p, hsa-miR-101-3p...

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Autores principales:	Veronica Filetti, Carla Loreto, Luca Falzone, Claudia Lombardo, Emanuele Cannizzaro, Sergio Castorina, Caterina Ledda, Venerando Rapisarda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fluoro-edenite asbestos malignant mesothelioma microRNA diagnosis prognosis Medicine R
Acceso en línea:	https://doaj.org/article/c9f278254ef445bbabcb04ead1baf514
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spelling	oai:doaj.org-article:c9f278254ef445bbabcb04ead1baf5142021-11-25T18:08:00ZDiagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma10.3390/jpm111112052075-4426https://doaj.org/article/c9f278254ef445bbabcb04ead1baf5142021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1205https://doaj.org/toc/2075-4426Fluoro-edenite (FE) is an asbestiform fiber identified in Biancavilla (Sicily, Italy). Environmental exposure to FE has been associated with a higher incidence of malignant mesothelioma (MM). The present study aimed to validate the predicted diagnostic significance of hsa-miR-323a-3p, hsa-miR-101-3p, and hsa-miR-20b-5p on a subset of MM patients exposed to FE and matched with healthy controls. For this purpose, MM tissues vs. nonmalignant pleura tissues were analyzed through droplet digital PCR (ddPCR) to evaluate differences in the expression levels of the selected miRNAs and their MM diagnostic potential. In addition, further computational analysis has been performed to establish the correlation of these miRNAs with the available online asbestos exposure data and clinic-pathological parameters to verify the potential role of these miRNAs as prognostic tools. ddPCR results showed that the three analyzed miRNAs were significantly down-regulated in MM cases vs. controls. Receiver operating characteristic (ROC) analysis revealed high specificity and sensitivity rates for both hsa-miR-323a-3p and hsa-miR-20b-5p, which thus acquire a diagnostic value for MM. In silico results showed a potential prognostic role of hsa-miR-101-3p due to a significant association of its higher expression and increased overall survival (OS) of MM patients.Veronica FilettiCarla LoretoLuca FalzoneClaudia LombardoEmanuele CannizzaroSergio CastorinaCaterina LeddaVenerando RapisardaMDPI AGarticlefluoro-edeniteasbestosmalignant mesotheliomamicroRNAdiagnosisprognosisMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1205, p 1205 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fluoro-edenite asbestos malignant mesothelioma microRNA diagnosis prognosis Medicine R
spellingShingle	fluoro-edenite asbestos malignant mesothelioma microRNA diagnosis prognosis Medicine R Veronica Filetti Carla Loreto Luca Falzone Claudia Lombardo Emanuele Cannizzaro Sergio Castorina Caterina Ledda Venerando Rapisarda Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
description	Fluoro-edenite (FE) is an asbestiform fiber identified in Biancavilla (Sicily, Italy). Environmental exposure to FE has been associated with a higher incidence of malignant mesothelioma (MM). The present study aimed to validate the predicted diagnostic significance of hsa-miR-323a-3p, hsa-miR-101-3p, and hsa-miR-20b-5p on a subset of MM patients exposed to FE and matched with healthy controls. For this purpose, MM tissues vs. nonmalignant pleura tissues were analyzed through droplet digital PCR (ddPCR) to evaluate differences in the expression levels of the selected miRNAs and their MM diagnostic potential. In addition, further computational analysis has been performed to establish the correlation of these miRNAs with the available online asbestos exposure data and clinic-pathological parameters to verify the potential role of these miRNAs as prognostic tools. ddPCR results showed that the three analyzed miRNAs were significantly down-regulated in MM cases vs. controls. Receiver operating characteristic (ROC) analysis revealed high specificity and sensitivity rates for both hsa-miR-323a-3p and hsa-miR-20b-5p, which thus acquire a diagnostic value for MM. In silico results showed a potential prognostic role of hsa-miR-101-3p due to a significant association of its higher expression and increased overall survival (OS) of MM patients.
format	article
author	Veronica Filetti Carla Loreto Luca Falzone Claudia Lombardo Emanuele Cannizzaro Sergio Castorina Caterina Ledda Venerando Rapisarda
author_facet	Veronica Filetti Carla Loreto Luca Falzone Claudia Lombardo Emanuele Cannizzaro Sergio Castorina Caterina Ledda Venerando Rapisarda
author_sort	Veronica Filetti
title	Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
title_short	Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
title_full	Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
title_fullStr	Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
title_full_unstemmed	Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma
title_sort	diagnostic and prognostic value of three micrornas in environmental asbestiform fibers-associated malignant mesothelioma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c9f278254ef445bbabcb04ead1baf514
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Diagnostic and Prognostic Value of Three microRNAs in Environmental Asbestiform Fibers-Associated Malignant Mesothelioma

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