A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis

Abstract Although accumulating evidence indicates that both β-catenin and osterix (Osx) are essential for bone and tooth development, few studies have investigated the interaction of these two key proteins in the context of cementogenesis. In this study, we used transgenic mice with constitutively a...

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Autores principales: Hwajung Choi, Tak-Heun Kim, Siqin Yang, Jeong-Chae Lee, Hyung-Keun You, Eui-Sic Cho
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/c9fb65efdaf94d9e805c11ba5ed1c5bc
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spelling oai:doaj.org-article:c9fb65efdaf94d9e805c11ba5ed1c5bc2021-12-02T11:52:44ZA Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis10.1038/s41598-017-08607-52045-2322https://doaj.org/article/c9fb65efdaf94d9e805c11ba5ed1c5bc2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08607-5https://doaj.org/toc/2045-2322Abstract Although accumulating evidence indicates that both β-catenin and osterix (Osx) are essential for bone and tooth development, few studies have investigated the interaction of these two key proteins in the context of cementogenesis. In this study, we used transgenic mice with constitutively active β-catenin and inactive Osx in the dental mesenchyme to address this question. We found that cementoblasts with constitutively active β-catenin require Osx to produce excessive cellular cementum, and that ablation of Osx prevents this abnormal accumulation. Importantly, cementoblasts transduced with retrovirus expressing constitutively active β-catenin exhibited upregulation of Osx expression through direct binding to the promoter region of Osx. Osx regulates Lef1 expression and consequently could regulate T-cell factor/lymphoid enhancer factor (Tcf/Lef) binding activity in Wnt/β-catenin signaling. However, the loss of Tcf/Lef binding activity by Osx ablation was not rescued by transduction of retrovirus expressing constitutively active β-catenin or ectopic Lef1 overexpression. These results suggest that the Tcf/Lef binding activity of Wnt/β-catenin signaling is Osx-dependent during cementogenesis. Moreover, Osx differentially regulates the expression of various Tcf family members, suggesting that Osx regulates cementogenesis by utilizing various Tcf/Lef-dependent mechanisms. This is the first report to show that downstream Osx signaling through Tcf/Lefs is critical for cementogenesis.Hwajung ChoiTak-Heun KimSiqin YangJeong-Chae LeeHyung-Keun YouEui-Sic ChoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hwajung Choi
Tak-Heun Kim
Siqin Yang
Jeong-Chae Lee
Hyung-Keun You
Eui-Sic Cho
A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
description Abstract Although accumulating evidence indicates that both β-catenin and osterix (Osx) are essential for bone and tooth development, few studies have investigated the interaction of these two key proteins in the context of cementogenesis. In this study, we used transgenic mice with constitutively active β-catenin and inactive Osx in the dental mesenchyme to address this question. We found that cementoblasts with constitutively active β-catenin require Osx to produce excessive cellular cementum, and that ablation of Osx prevents this abnormal accumulation. Importantly, cementoblasts transduced with retrovirus expressing constitutively active β-catenin exhibited upregulation of Osx expression through direct binding to the promoter region of Osx. Osx regulates Lef1 expression and consequently could regulate T-cell factor/lymphoid enhancer factor (Tcf/Lef) binding activity in Wnt/β-catenin signaling. However, the loss of Tcf/Lef binding activity by Osx ablation was not rescued by transduction of retrovirus expressing constitutively active β-catenin or ectopic Lef1 overexpression. These results suggest that the Tcf/Lef binding activity of Wnt/β-catenin signaling is Osx-dependent during cementogenesis. Moreover, Osx differentially regulates the expression of various Tcf family members, suggesting that Osx regulates cementogenesis by utilizing various Tcf/Lef-dependent mechanisms. This is the first report to show that downstream Osx signaling through Tcf/Lefs is critical for cementogenesis.
format article
author Hwajung Choi
Tak-Heun Kim
Siqin Yang
Jeong-Chae Lee
Hyung-Keun You
Eui-Sic Cho
author_facet Hwajung Choi
Tak-Heun Kim
Siqin Yang
Jeong-Chae Lee
Hyung-Keun You
Eui-Sic Cho
author_sort Hwajung Choi
title A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
title_short A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
title_full A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
title_fullStr A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
title_full_unstemmed A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
title_sort reciprocal interaction between β-catenin and osterix in cementogenesis
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/c9fb65efdaf94d9e805c11ba5ed1c5bc
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