A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies

Summary: The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon i...

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Autores principales:	Gabriel A. Russell, Garrie Peng, Cynthia Faubert, Elena F. Verdu, Siegfried Hapfelmeier, Irah L. King
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell culture Health Sciences Immunology Microbiology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/ca0c8ffe12dd4c24b19f7de13bf355a5
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spelling	oai:doaj.org-article:ca0c8ffe12dd4c24b19f7de13bf355a52021-11-12T04:47:48ZA protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies2666-166710.1016/j.xpro.2021.100946https://doaj.org/article/ca0c8ffe12dd4c24b19f7de13bf355a52021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006523https://doaj.org/toc/2666-1667Summary: The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.Gabriel A. RussellGarrie PengCynthia FaubertElena F. VerduSiegfried HapfelmeierIrah L. KingElsevierarticleCell cultureHealth SciencesImmunologyMicrobiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100946- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell culture Health Sciences Immunology Microbiology Science (General) Q1-390
spellingShingle	Cell culture Health Sciences Immunology Microbiology Science (General) Q1-390 Gabriel A. Russell Garrie Peng Cynthia Faubert Elena F. Verdu Siegfried Hapfelmeier Irah L. King A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
description	Summary: The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
format	article
author	Gabriel A. Russell Garrie Peng Cynthia Faubert Elena F. Verdu Siegfried Hapfelmeier Irah L. King
author_facet	Gabriel A. Russell Garrie Peng Cynthia Faubert Elena F. Verdu Siegfried Hapfelmeier Irah L. King
author_sort	Gabriel A. Russell
title	A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_short	A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_full	A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_fullStr	A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_full_unstemmed	A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_sort	protocol for generating germ-free heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ca0c8ffe12dd4c24b19f7de13bf355a5
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A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies

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