Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals

The aim of the present paper is to assess the relationship between oxysterol levels and retinal microvascular function in individuals of various age groups, free of clinically evident diseases. Forty-two apparently healthy individuals were included in the present study (group 1: 19–30 years, group 2...

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Autores principales:	Hala Shokr, Irundika HK Dias, Doina Gherghel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oxidative stress oxysterols cardiovascular disease risk Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ca0f6fa7aa6c4aefab0ba40ff4c7802e
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spelling	oai:doaj.org-article:ca0f6fa7aa6c4aefab0ba40ff4c7802e2021-11-25T16:27:54ZOxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals10.3390/antiox101117562076-3921https://doaj.org/article/ca0f6fa7aa6c4aefab0ba40ff4c7802e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1756https://doaj.org/toc/2076-3921The aim of the present paper is to assess the relationship between oxysterol levels and retinal microvascular function in individuals of various age groups, free of clinically evident diseases. Forty-two apparently healthy individuals were included in the present study (group 1: 19–30 years, group 2: 31–50 years, and group 3: 51–70 years). Retinal microvascular function was assessed using the dynamic retinal vessel analyzer (DVA, IMEDOS GmbH, Jena, Germany). Fasting plasma was obtained from all subjects and quantification of monohydroxy and dihydroxy oxysterols assessment was performed using LC-MS/MS following reverse phase chromatography. A Griess assay was used to evaluate the Nitric Oxide (NO) concentration in all individuals. The glutathione redox ratio was also analyzed by means of whole blood glutathione recycling assay. In all participants, the levels of 7-Ketocholesterol, 25-hydroxycholesterol and 7β-hydroxycholesterol correlated significantly and positively with the time to maximum arteriolar dilation. In addition, 25-hydroxycholesterol and 7β-hydroxycholesterol negatively correlated to the percentage of maximum arteriolar dilation. A negative correlation was observed for 27-hydroxycholesterol and 7β-hydroxycholesterol with microvascular arteriolar constriction. These results suggest that, with age, abnormal oxysterol levels correlate with early changes in microvascular bed function. This relationship could signal early risk for cardiovascular diseases (CVDs) in an ageing population.Hala ShokrIrundika HK DiasDoina GherghelMDPI AGarticleoxidative stressoxysterolscardiovascular disease riskTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1756, p 1756 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress oxysterols cardiovascular disease risk Therapeutics. Pharmacology RM1-950
spellingShingle	oxidative stress oxysterols cardiovascular disease risk Therapeutics. Pharmacology RM1-950 Hala Shokr Irundika HK Dias Doina Gherghel Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
description	The aim of the present paper is to assess the relationship between oxysterol levels and retinal microvascular function in individuals of various age groups, free of clinically evident diseases. Forty-two apparently healthy individuals were included in the present study (group 1: 19–30 years, group 2: 31–50 years, and group 3: 51–70 years). Retinal microvascular function was assessed using the dynamic retinal vessel analyzer (DVA, IMEDOS GmbH, Jena, Germany). Fasting plasma was obtained from all subjects and quantification of monohydroxy and dihydroxy oxysterols assessment was performed using LC-MS/MS following reverse phase chromatography. A Griess assay was used to evaluate the Nitric Oxide (NO) concentration in all individuals. The glutathione redox ratio was also analyzed by means of whole blood glutathione recycling assay. In all participants, the levels of 7-Ketocholesterol, 25-hydroxycholesterol and 7β-hydroxycholesterol correlated significantly and positively with the time to maximum arteriolar dilation. In addition, 25-hydroxycholesterol and 7β-hydroxycholesterol negatively correlated to the percentage of maximum arteriolar dilation. A negative correlation was observed for 27-hydroxycholesterol and 7β-hydroxycholesterol with microvascular arteriolar constriction. These results suggest that, with age, abnormal oxysterol levels correlate with early changes in microvascular bed function. This relationship could signal early risk for cardiovascular diseases (CVDs) in an ageing population.
format	article
author	Hala Shokr Irundika HK Dias Doina Gherghel
author_facet	Hala Shokr Irundika HK Dias Doina Gherghel
author_sort	Hala Shokr
title	Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
title_short	Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
title_full	Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
title_fullStr	Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
title_full_unstemmed	Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals
title_sort	oxysterols and retinal microvascular dysfunction as early risk markers for cardiovascular disease in normal, ageing individuals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ca0f6fa7aa6c4aefab0ba40ff4c7802e
work_keys_str_mv	AT halashokr oxysterolsandretinalmicrovasculardysfunctionasearlyriskmarkersforcardiovasculardiseaseinnormalageingindividuals AT irundikahkdias oxysterolsandretinalmicrovasculardysfunctionasearlyriskmarkersforcardiovasculardiseaseinnormalageingindividuals AT doinagherghel oxysterolsandretinalmicrovasculardysfunctionasearlyriskmarkersforcardiovasculardiseaseinnormalageingindividuals
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Oxysterols and Retinal Microvascular Dysfunction as Early Risk Markers for Cardiovascular Disease in Normal, Ageing Individuals

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