One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons

Abstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decrea...

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Autores principales:	Maude Gerbaix, Vasily Gnyubkin, Delphine Farlay, Cécile Olivier, Patrick Ammann, Guillaume Courbon, Norbert Laroche, Rachel Genthial, Hélène Follet, Françoise Peyrin, Boris Shenkman, Guillemette Gauquelin-Koch, Laurence Vico
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca18ee232ea8420da5ef90bbd29606f1
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spelling	oai:doaj.org-article:ca18ee232ea8420da5ef90bbd29606f12021-12-02T12:30:36ZOne-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons10.1038/s41598-017-03014-22045-2322https://doaj.org/article/ca18ee232ea8420da5ef90bbd29606f12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03014-2https://doaj.org/toc/2045-2322Abstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decreased the trabecular bone volume (−64% vs. Habitat Control), dramatically increased the bone resorption (+140% vs. Habitat Control) and induced marrow adiposity invasion. At the diaphysis, cortical thinning associated with periosteal resorption was observed. In the Flight animal group, the osteocyte lacunae displayed a reduced volume and a more spherical shape (synchrotron radiation analyses), and empty lacunae were highly increased (+344% vs. Habitat Control). Tissue-level mechanical cortical properties (i.e., hardness and modulus) were locally decreased by spaceflight, whereas the mineral characteristics and collagen maturity were unaffected. In the vertebrae, spaceflight decreased the overall bone volume and altered the modulus in the periphery of the trabecular struts. Despite normalized osteoclastic activity and an increased osteoblast number, bone recovery was not observed 8 days after landing. In conclusion, spaceflight induces osteocyte death, which may trigger bone resorption and result in bone mass and microstructural deterioration. Moreover, osteocyte cell death, lacunae mineralization and fatty marrow, which are hallmarks of ageing, may impede tissue maintenance and repair.Maude GerbaixVasily GnyubkinDelphine FarlayCécile OlivierPatrick AmmannGuillaume CourbonNorbert LarocheRachel GenthialHélène FolletFrançoise PeyrinBoris ShenkmanGuillemette Gauquelin-KochLaurence VicoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
description	Abstract The weightless environment during spaceflight induces site-specific bone loss. The 30-day Bion-M1 mission offered a unique opportunity to characterize the skeletal changes after spaceflight and an 8-day recovery period in mature male C57/BL6 mice. In the femur metaphysis, spaceflight decreased the trabecular bone volume (−64% vs. Habitat Control), dramatically increased the bone resorption (+140% vs. Habitat Control) and induced marrow adiposity invasion. At the diaphysis, cortical thinning associated with periosteal resorption was observed. In the Flight animal group, the osteocyte lacunae displayed a reduced volume and a more spherical shape (synchrotron radiation analyses), and empty lacunae were highly increased (+344% vs. Habitat Control). Tissue-level mechanical cortical properties (i.e., hardness and modulus) were locally decreased by spaceflight, whereas the mineral characteristics and collagen maturity were unaffected. In the vertebrae, spaceflight decreased the overall bone volume and altered the modulus in the periphery of the trabecular struts. Despite normalized osteoclastic activity and an increased osteoblast number, bone recovery was not observed 8 days after landing. In conclusion, spaceflight induces osteocyte death, which may trigger bone resorption and result in bone mass and microstructural deterioration. Moreover, osteocyte cell death, lacunae mineralization and fatty marrow, which are hallmarks of ageing, may impede tissue maintenance and repair.
format	article
author	Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico
author_facet	Maude Gerbaix Vasily Gnyubkin Delphine Farlay Cécile Olivier Patrick Ammann Guillaume Courbon Norbert Laroche Rachel Genthial Hélène Follet Françoise Peyrin Boris Shenkman Guillemette Gauquelin-Koch Laurence Vico
author_sort	Maude Gerbaix
title	One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
title_short	One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
title_full	One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
title_fullStr	One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
title_full_unstemmed	One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
title_sort	one-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ca18ee232ea8420da5ef90bbd29606f1
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One-month spaceflight compromises the bone microstructure, tissue-level mechanical properties, osteocyte survival and lacunae volume in mature mice skeletons

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