Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity

Peihao Yin,1,* Yan Wang,1,* YanYan Qiu,1 LiLi Hou,1 Xuan Liu,1 Jianmin Qin,1 Yourong Duan,2 Peifeng Liu,2 Ming Qiu,3 Qi Li11Department of Clinical Oncology, Putuo Hospital and Interventional Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, Chin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Duan YR, Liu PF, Qiu M, Li Q, Yin PH, Wang Y, Qiu YY, Hou LL, Liu X, Qin JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca192fe9053f41bb838fccf18695699d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ca192fe9053f41bb838fccf18695699d
record_format	dspace
spelling	oai:doaj.org-article:ca192fe9053f41bb838fccf18695699d2021-12-02T02:42:26ZBufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity1176-91141178-2013https://doaj.org/article/ca192fe9053f41bb838fccf18695699d2012-07-01T00:00:00Zhttp://www.dovepress.com/bufalin-loaded-mpeg-plga-pll-crgd-nanoparticles-preparation-cellular-u-a10531https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Peihao Yin,1,* Yan Wang,1,* YanYan Qiu,1 LiLi Hou,1 Xuan Liu,1 Jianmin Qin,1 Yourong Duan,2 Peifeng Liu,2 Ming Qiu,3 Qi Li11Department of Clinical Oncology, Putuo Hospital and Interventional Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Shanghai Cancer Institute, Jiaotong University, Shanghai, China; 3Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China *These authors contributed equally to this workBackground: Recent studies have shown that bufalin has a good antitumor effect but has high toxicity, poor water solubility, a short half-life, a narrow therapeutic window, and a toxic dose that is close to the therapeutic dose, which all limit its clinical application. This study aimed to determine the targeting efficacy of nanoparticles (NPs) made of methoxy polyethylene glycol (mPEG), polylactic-co-glycolic acid (PLGA), poly-L-lysine (PLL), and cyclic arginine-glycine-aspartic acid (cRGD) loaded with bufalin, ie, bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles (BNPs), in SW620 colon cancer-bearing mice.Methods: BNPs showed uniform size. The size, shape, zeta potential, drug loading, encapsulation efficiency, and release of these nanoparticles were studied in vitro. The tumor targeting, cellular uptake, and growth-inhibitory effect of BNPs in vivo were tested.Results: BNPs were of uniform size with an average particle size of 164 &plusmn; 84 nm and zeta potential of 2.77 mV. The encapsulation efficiency was 81.7% &plusmn; 0.89%, and the drug load was 3.92% &plusmn; 0.16%. The results of in vitro cytotoxicity studies showed that although the blank NPs were nontoxic, they enhanced the cytotoxicity of bufalin in BNPs. Drug release experiments showed that the release of the drug was prolonged and sustained. The results of confocal laser scanning microscopy indicated that BNPs could effectively bind to human umbilical vein endothelial cells. In the SW620 xenograft mice model, the BNPs could effectively target the tumor in vivo. The BNPs were significantly more effective than other NPs in preventing tumor growth.Conclusion: BNPs had even size distribution, were stable, and had a slow-releasing and tumor-targeting effect. BNPs significantly inhibited colon cancer growth in vitro and in vivo. As a novel drug carrier system, BNPs are a potentially promising targeting treatment for colon cancer.Keywords: colon cancer, nanoparticles, tumor target, bufalinDuan YRLiu PFQiu MLi QYin PHWang YQiu YYHou LLLiu XQin JMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3961-3969 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Duan YR Liu PF Qiu M Li Q Yin PH Wang Y Qiu YY Hou LL Liu X Qin JM Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
description	Peihao Yin,1,* Yan Wang,1,* YanYan Qiu,1 LiLi Hou,1 Xuan Liu,1 Jianmin Qin,1 Yourong Duan,2 Peifeng Liu,2 Ming Qiu,3 Qi Li11Department of Clinical Oncology, Putuo Hospital and Interventional Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Shanghai Cancer Institute, Jiaotong University, Shanghai, China; 3Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China *These authors contributed equally to this workBackground: Recent studies have shown that bufalin has a good antitumor effect but has high toxicity, poor water solubility, a short half-life, a narrow therapeutic window, and a toxic dose that is close to the therapeutic dose, which all limit its clinical application. This study aimed to determine the targeting efficacy of nanoparticles (NPs) made of methoxy polyethylene glycol (mPEG), polylactic-co-glycolic acid (PLGA), poly-L-lysine (PLL), and cyclic arginine-glycine-aspartic acid (cRGD) loaded with bufalin, ie, bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles (BNPs), in SW620 colon cancer-bearing mice.Methods: BNPs showed uniform size. The size, shape, zeta potential, drug loading, encapsulation efficiency, and release of these nanoparticles were studied in vitro. The tumor targeting, cellular uptake, and growth-inhibitory effect of BNPs in vivo were tested.Results: BNPs were of uniform size with an average particle size of 164 &plusmn; 84 nm and zeta potential of 2.77 mV. The encapsulation efficiency was 81.7% &plusmn; 0.89%, and the drug load was 3.92% &plusmn; 0.16%. The results of in vitro cytotoxicity studies showed that although the blank NPs were nontoxic, they enhanced the cytotoxicity of bufalin in BNPs. Drug release experiments showed that the release of the drug was prolonged and sustained. The results of confocal laser scanning microscopy indicated that BNPs could effectively bind to human umbilical vein endothelial cells. In the SW620 xenograft mice model, the BNPs could effectively target the tumor in vivo. The BNPs were significantly more effective than other NPs in preventing tumor growth.Conclusion: BNPs had even size distribution, were stable, and had a slow-releasing and tumor-targeting effect. BNPs significantly inhibited colon cancer growth in vitro and in vivo. As a novel drug carrier system, BNPs are a potentially promising targeting treatment for colon cancer.Keywords: colon cancer, nanoparticles, tumor target, bufalin
format	article
author	Duan YR Liu PF Qiu M Li Q Yin PH Wang Y Qiu YY Hou LL Liu X Qin JM
author_facet	Duan YR Liu PF Qiu M Li Q Yin PH Wang Y Qiu YY Hou LL Liu X Qin JM
author_sort	Duan YR
title	Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
title_short	Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
title_full	Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
title_fullStr	Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
title_full_unstemmed	Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
title_sort	bufalin-loaded mpeg-plga-pll-crgd nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ca192fe9053f41bb838fccf18695699d
work_keys_str_mv	AT duanyr bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT liupf bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT qium bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT liq bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT yinph bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT wangy bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT qiuyy bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT houll bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT liux bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity AT qinjm bufalinloadedmpegplgapllcrgdnanoparticlespreparationcellularuptaketissuedistributionandanticanceractivity
_version_	1718402250202677248

Bufalin-loaded mPEG-PLGA-PLL-cRGD nanoparticles: preparation, cellular uptake, tissue distribution, and anticancer activity

Ejemplares similares