Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA

Teegala Lakshminarayan Reddy,1,5 P Sivarama Krishnarao,2 Garikapati Koteswara Rao,1,5 Eswar Bhimireddy,2 P Venkateswarlu,4 Debendra K Mohapatra,2,5 JS Yadav,2 Utpal Bhadra,3 Manika Pal Bhadra1,5 1Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, 2Natural Products Chemistry D...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Reddy TL, Krishnarao PS, Rao GK, Bhimireddy E, Venkateswarlu P, Mohapatra DK, Yadav JS, Bhadra U, Pal Bhadra M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca1f931a209a4a428f8edb81e465fed4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ca1f931a209a4a428f8edb81e465fed4
record_format	dspace
spelling	oai:doaj.org-article:ca1f931a209a4a428f8edb81e465fed42021-12-02T03:58:30ZPara amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA1178-2013https://doaj.org/article/ca1f931a209a4a428f8edb81e465fed42015-10-01T00:00:00Zhttps://www.dovepress.com/para-amino-benzoic-acid-derived-self-assembled-biocompatible-nanoparti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Teegala Lakshminarayan Reddy,1,5 P Sivarama Krishnarao,2 Garikapati Koteswara Rao,1,5 Eswar Bhimireddy,2 P Venkateswarlu,4 Debendra K Mohapatra,2,5 JS Yadav,2 Utpal Bhadra,3 Manika Pal Bhadra1,5 1Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, 2Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, 3Functional Genomics and Gene Silencing Group, CSIR-Indian Institute of Chemical Technology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, 4Department of Chemistry, Sri Venkateswara University, Tirpupati, 5Academy of Scientific and Innovative Research, New Delhi, India Abstract: A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery. Keywords: biocompatibility, nanoparticles, siRNA, cell uptake, endocytosis, gene silencingReddy TLKrishnarao PSRao GKBhimireddy EVenkateswarlu PMohapatra DKYadav JSBhadra UPal Bhadra MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6411-6424 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Reddy TL Krishnarao PS Rao GK Bhimireddy E Venkateswarlu P Mohapatra DK Yadav JS Bhadra U Pal Bhadra M Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
description	Teegala Lakshminarayan Reddy,1,5 P Sivarama Krishnarao,2 Garikapati Koteswara Rao,1,5 Eswar Bhimireddy,2 P Venkateswarlu,4 Debendra K Mohapatra,2,5 JS Yadav,2 Utpal Bhadra,3 Manika Pal Bhadra1,5 1Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, 2Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, 3Functional Genomics and Gene Silencing Group, CSIR-Indian Institute of Chemical Technology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, 4Department of Chemistry, Sri Venkateswara University, Tirpupati, 5Academy of Scientific and Innovative Research, New Delhi, India Abstract: A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery. Keywords: biocompatibility, nanoparticles, siRNA, cell uptake, endocytosis, gene silencing
format	article
author	Reddy TL Krishnarao PS Rao GK Bhimireddy E Venkateswarlu P Mohapatra DK Yadav JS Bhadra U Pal Bhadra M
author_facet	Reddy TL Krishnarao PS Rao GK Bhimireddy E Venkateswarlu P Mohapatra DK Yadav JS Bhadra U Pal Bhadra M
author_sort	Reddy TL
title	Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_short	Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_full	Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_fullStr	Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_full_unstemmed	Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_sort	para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of sirna
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/ca1f931a209a4a428f8edb81e465fed4
work_keys_str_mv	AT reddytl paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT krishnaraops paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT raogk paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT bhimireddye paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT venkateswarlup paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT mohapatradk paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT yadavjs paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT bhadrau paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna AT palbhadram paraaminobenzoicacidderivedselfassembledbiocompatiblenanoparticlesforefficientdeliveryofsirna
_version_	1718401517748224000

Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA

Ejemplares similares