Voltage-Gated Lipid Ion Channels.

Synthetic lipid membranes can display channel-like ion conduction events even in the absence of proteins. We show here that these events are voltage-gated with a quadratic voltage dependence as expected from electrostatic theory of capacitors. To this end, we recorded channel traces and current hist...

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Autores principales:	Andreas Blicher, Thomas Heimburg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca22d399b65c4768b2a2b3cea471d599
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spelling	oai:doaj.org-article:ca22d399b65c4768b2a2b3cea471d5992021-11-18T07:41:28ZVoltage-Gated Lipid Ion Channels.1932-620310.1371/journal.pone.0065707https://doaj.org/article/ca22d399b65c4768b2a2b3cea471d5992013-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0065707https://doaj.org/toc/1932-6203Synthetic lipid membranes can display channel-like ion conduction events even in the absence of proteins. We show here that these events are voltage-gated with a quadratic voltage dependence as expected from electrostatic theory of capacitors. To this end, we recorded channel traces and current histograms in patch-experiments on lipid membranes. We derived a theoretical current-voltage relationship for pores in lipid membranes that describes the experimental data very well when assuming an asymmetric membrane. We determined the equilibrium constant between closed and open state and the open probability as a function of voltage. The voltage-dependence of the lipid pores is found comparable to that of protein channels. Lifetime distributions of open and closed events indicate that the channel open distribution does not follow exponential statistics but rather power law behavior for long open times.Andreas BlicherThomas HeimburgPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65707 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreas Blicher Thomas Heimburg Voltage-Gated Lipid Ion Channels.
description	Synthetic lipid membranes can display channel-like ion conduction events even in the absence of proteins. We show here that these events are voltage-gated with a quadratic voltage dependence as expected from electrostatic theory of capacitors. To this end, we recorded channel traces and current histograms in patch-experiments on lipid membranes. We derived a theoretical current-voltage relationship for pores in lipid membranes that describes the experimental data very well when assuming an asymmetric membrane. We determined the equilibrium constant between closed and open state and the open probability as a function of voltage. The voltage-dependence of the lipid pores is found comparable to that of protein channels. Lifetime distributions of open and closed events indicate that the channel open distribution does not follow exponential statistics but rather power law behavior for long open times.
format	article
author	Andreas Blicher Thomas Heimburg
author_facet	Andreas Blicher Thomas Heimburg
author_sort	Andreas Blicher
title	Voltage-Gated Lipid Ion Channels.
title_short	Voltage-Gated Lipid Ion Channels.
title_full	Voltage-Gated Lipid Ion Channels.
title_fullStr	Voltage-Gated Lipid Ion Channels.
title_full_unstemmed	Voltage-Gated Lipid Ion Channels.
title_sort	voltage-gated lipid ion channels.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ca22d399b65c4768b2a2b3cea471d599
work_keys_str_mv	AT andreasblicher voltagegatedlipidionchannels AT thomasheimburg voltagegatedlipidionchannels
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Voltage-Gated Lipid Ion Channels.

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