Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)

This study describes for the first time the comprehensive characterization of tetrapyrrole cofactor biosynthetic pathways developed for bacterial community (BC) inhabiting shale rock. Based on the genomic and proteomic metadata, we have detailed the biosynthesis of siroheme, heme, cobalamin, and the...

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Autores principales:	Robert Stasiuk, Tomasz Krucoń, Renata Matlakowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	porphyrin heme siroheme cobalamin shale rock bacteria Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ca233ef2036e42c38f7d9213e714f6df
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spelling	oai:doaj.org-article:ca233ef2036e42c38f7d9213e714f6df2021-11-11T18:40:34ZBiosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)10.3390/molecules262167461420-3049https://doaj.org/article/ca233ef2036e42c38f7d9213e714f6df2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6746https://doaj.org/toc/1420-3049This study describes for the first time the comprehensive characterization of tetrapyrrole cofactor biosynthetic pathways developed for bacterial community (BC) inhabiting shale rock. Based on the genomic and proteomic metadata, we have detailed the biosynthesis of siroheme, heme, cobalamin, and the major precursor uroporphyrinogen III by a deep BC living on a rock containing sedimentary tetrapyrrole compounds. The obtained results showed the presence of incomplete heme and cobalamin biosynthesis pathways in the studied BC. At the same time, the production of proteins containing these cofactors, such as cytochromes, catalases and sulfite reductase, was observed. The results obtained are crucial for understanding the ecology of bacteria inhabiting shale rock, as well as their metabolism and potential impact on the biogeochemistry of these rocks. Based on the findings, we hypothesize that the bacteria may use primary or modified sedimentary porphyrins and their degradation products as precursors for synthesizing tetrapyrrole cofactors. Experimental testing of this hypothesis is of course necessary, but its evidence would point to an important and unique phenomenon of the tetrapyrrole ring cycle on Earth involving bacteria.Robert StasiukTomasz KrucońRenata MatlakowskaMDPI AGarticleporphyrinhemesirohemecobalaminshale rockbacteriaOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6746, p 6746 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	porphyrin heme siroheme cobalamin shale rock bacteria Organic chemistry QD241-441
spellingShingle	porphyrin heme siroheme cobalamin shale rock bacteria Organic chemistry QD241-441 Robert Stasiuk Tomasz Krucoń Renata Matlakowska Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
description	This study describes for the first time the comprehensive characterization of tetrapyrrole cofactor biosynthetic pathways developed for bacterial community (BC) inhabiting shale rock. Based on the genomic and proteomic metadata, we have detailed the biosynthesis of siroheme, heme, cobalamin, and the major precursor uroporphyrinogen III by a deep BC living on a rock containing sedimentary tetrapyrrole compounds. The obtained results showed the presence of incomplete heme and cobalamin biosynthesis pathways in the studied BC. At the same time, the production of proteins containing these cofactors, such as cytochromes, catalases and sulfite reductase, was observed. The results obtained are crucial for understanding the ecology of bacteria inhabiting shale rock, as well as their metabolism and potential impact on the biogeochemistry of these rocks. Based on the findings, we hypothesize that the bacteria may use primary or modified sedimentary porphyrins and their degradation products as precursors for synthesizing tetrapyrrole cofactors. Experimental testing of this hypothesis is of course necessary, but its evidence would point to an important and unique phenomenon of the tetrapyrrole ring cycle on Earth involving bacteria.
format	article
author	Robert Stasiuk Tomasz Krucoń Renata Matlakowska
author_facet	Robert Stasiuk Tomasz Krucoń Renata Matlakowska
author_sort	Robert Stasiuk
title	Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
title_short	Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
title_full	Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
title_fullStr	Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
title_full_unstemmed	Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)
title_sort	biosynthesis of tetrapyrrole cofactors by bacterial community inhabiting porphyrine-containing shale rock (fore-sudetic monocline)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ca233ef2036e42c38f7d9213e714f6df
work_keys_str_mv	AT robertstasiuk biosynthesisoftetrapyrrolecofactorsbybacterialcommunityinhabitingporphyrinecontainingshalerockforesudeticmonocline AT tomaszkrucon biosynthesisoftetrapyrrolecofactorsbybacterialcommunityinhabitingporphyrinecontainingshalerockforesudeticmonocline AT renatamatlakowska biosynthesisoftetrapyrrolecofactorsbybacterialcommunityinhabitingporphyrinecontainingshalerockforesudeticmonocline
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Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)

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