Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment

Ensieh Ghasemian Lemraski,1 Hossein Jahangirian,2 Maryam Dashti,1 Elaheh Khajehali,3 Soheila Sharafinia,4 Roshanak Rafiee-Moghaddam,2 Thomas J Webster2 1Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran; 2Department of Chemical Engineering, Northeastern University, Boston, MA...

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Autores principales:	Ghasemian Lemraski E, Jahangirian H, Dashti M, Khajehali E, Sharafinia S, Rafiee-Moghaddam R, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	antimicrobial healing hydrogel copper nanofiber chitosan Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca285e59a2794a07a66be844ee2974e6
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spelling	oai:doaj.org-article:ca285e59a2794a07a66be844ee2974e62021-12-02T13:36:08ZAntimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment1178-2013https://doaj.org/article/ca285e59a2794a07a66be844ee2974e62021-01-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-double-layer-wound-dressing-based-on-chitosanpolyvinyl-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ensieh Ghasemian Lemraski,1 Hossein Jahangirian,2 Maryam Dashti,1 Elaheh Khajehali,3 Soheila Sharafinia,4 Roshanak Rafiee-Moghaddam,2 Thomas J Webster2 1Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran; 2Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 3Department of Food Hygiene, Faculty of Veterinary Medicine, Ilam University, Ilam, Iran; 4Department of Chemistry Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranCorrespondence: Ensieh Ghasemian Lemraski; Hossein Jahangirian Email e.ghasemian@ilam.ac.ir; kamran.jahangirian@gmail.comPurpose: Today, the development of wounds and their side effects has become a problematic issue in medical science research. Hydrogel-based dressings are some of the best candidates for this purpose due to their ability to keep the wound bed clean, as well as provide proper moisture, tissue compatibility and an antimicrobial effect for wound healing. On the other hand, copper and its compounds have been used experimentally for many years in studies as an antimicrobial substance. Various studies have been performed determining the antimicrobial properties of this element, during which significant effects on infection have been shown.Methods: Chitosan/polyvinyl alcohol/copper nanofibers were successfully prepared by incorporating Cu onto a polymer electrospun using an electrospinning technique. A double-layer nanofiber composed of poly(vinyl alcohol) and chitosan incorporated with Cu nanoparticles as a protective layer and a second layer composed of polyvinylpyrrolidone (PVP) nanofibers which was adjacent to the damaged cells was prepared. The prepared nanofiber was characterized by TGA, FT-IR, TEM, SEM-EDS, and X-ray powder diffraction. The antimicrobial efficiency of the nanofibers was demonstrated through biological tests on some Gram-positive and Gram-negative bacteria. Finally, the prepared hydrogel formulations were prepared to evaluate their effect on the healing process of rat open wounds.Results: In this study, data from SEM, TEM, EDS, and XRD confirmed the formation of uniform fibers with nanodiameters and the presence of Cu nanoparticles onto the electrospun nanofibers. The antibacterial activity of copper was observed against all of the selected bacteria, but the Gram-positive bacteria were more sensitive compared to Gram-negative bacteria.Conclusion: According to the obtained results, the hydrogel wound dressing prepared in this research can be effective in caring for open wounds in the early stages of wound healing and preventing the occurrence of prolonged open wounds.Keywords: antimicrobial, healing, hydrogel, copper, nanofiber, chitosanGhasemian Lemraski EJahangirian HDashti MKhajehali ESharafinia SRafiee-Moghaddam RWebster TJDove Medical PressarticleantimicrobialhealinghydrogelcoppernanofiberchitosanMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 223-235 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial healing hydrogel copper nanofiber chitosan Medicine (General) R5-920
spellingShingle	antimicrobial healing hydrogel copper nanofiber chitosan Medicine (General) R5-920 Ghasemian Lemraski E Jahangirian H Dashti M Khajehali E Sharafinia S Rafiee-Moghaddam R Webster TJ Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
description	Ensieh Ghasemian Lemraski,1 Hossein Jahangirian,2 Maryam Dashti,1 Elaheh Khajehali,3 Soheila Sharafinia,4 Roshanak Rafiee-Moghaddam,2 Thomas J Webster2 1Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran; 2Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 3Department of Food Hygiene, Faculty of Veterinary Medicine, Ilam University, Ilam, Iran; 4Department of Chemistry Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranCorrespondence: Ensieh Ghasemian Lemraski; Hossein Jahangirian Email e.ghasemian@ilam.ac.ir; kamran.jahangirian@gmail.comPurpose: Today, the development of wounds and their side effects has become a problematic issue in medical science research. Hydrogel-based dressings are some of the best candidates for this purpose due to their ability to keep the wound bed clean, as well as provide proper moisture, tissue compatibility and an antimicrobial effect for wound healing. On the other hand, copper and its compounds have been used experimentally for many years in studies as an antimicrobial substance. Various studies have been performed determining the antimicrobial properties of this element, during which significant effects on infection have been shown.Methods: Chitosan/polyvinyl alcohol/copper nanofibers were successfully prepared by incorporating Cu onto a polymer electrospun using an electrospinning technique. A double-layer nanofiber composed of poly(vinyl alcohol) and chitosan incorporated with Cu nanoparticles as a protective layer and a second layer composed of polyvinylpyrrolidone (PVP) nanofibers which was adjacent to the damaged cells was prepared. The prepared nanofiber was characterized by TGA, FT-IR, TEM, SEM-EDS, and X-ray powder diffraction. The antimicrobial efficiency of the nanofibers was demonstrated through biological tests on some Gram-positive and Gram-negative bacteria. Finally, the prepared hydrogel formulations were prepared to evaluate their effect on the healing process of rat open wounds.Results: In this study, data from SEM, TEM, EDS, and XRD confirmed the formation of uniform fibers with nanodiameters and the presence of Cu nanoparticles onto the electrospun nanofibers. The antibacterial activity of copper was observed against all of the selected bacteria, but the Gram-positive bacteria were more sensitive compared to Gram-negative bacteria.Conclusion: According to the obtained results, the hydrogel wound dressing prepared in this research can be effective in caring for open wounds in the early stages of wound healing and preventing the occurrence of prolonged open wounds.Keywords: antimicrobial, healing, hydrogel, copper, nanofiber, chitosan
format	article
author	Ghasemian Lemraski E Jahangirian H Dashti M Khajehali E Sharafinia S Rafiee-Moghaddam R Webster TJ
author_facet	Ghasemian Lemraski E Jahangirian H Dashti M Khajehali E Sharafinia S Rafiee-Moghaddam R Webster TJ
author_sort	Ghasemian Lemraski E
title	Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
title_short	Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
title_full	Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
title_fullStr	Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
title_full_unstemmed	Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
title_sort	antimicrobial double-layer wound dressing based on chitosan/polyvinyl alcohol/copper: in vitro and in vivo assessment
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/ca285e59a2794a07a66be844ee2974e6
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Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment

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