Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia

Retnowati Y, Moeljopawiro S, Djohan TjS, Soetarto ES. 2018. Antimicrobial activities of actinomycetes isolates from rhizospheric soil on different mangrove forests of Torosiaje, Gorontalo, Indonesia. Biodiversitas 19: 2196-2203. Mangrove forests are very productive ecosystems that form unique saline...

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Autores principales:	YULIANA RETNOWATI, SUKARTI MOELJOPAWIRO, TJUT SUGANDAWATY DJOHAN, ENDANG SUTARININGSIH SOETARTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	actinomycetes antibiotics mangrove forest rhizospheric soil Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ca2ee0a67fb74e308ec7c7cb04edc0b7
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spelling	oai:doaj.org-article:ca2ee0a67fb74e308ec7c7cb04edc0b72021-11-16T14:04:40ZAntimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia1412-033X2085-472210.13057/biodiv/d190627https://doaj.org/article/ca2ee0a67fb74e308ec7c7cb04edc0b72018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3112https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Retnowati Y, Moeljopawiro S, Djohan TjS, Soetarto ES. 2018. Antimicrobial activities of actinomycetes isolates from rhizospheric soil on different mangrove forests of Torosiaje, Gorontalo, Indonesia. Biodiversitas 19: 2196-2203. Mangrove forests are very productive ecosystems that form unique saline environment very rich in organic matter, containing nitrogen and sulfur available for microorganisms. Mangrove forest as an extreme environment is promising to be sources of antibiotic-producing actinomycetes. The objectives of this study were to analyze the antimicrobial activities of metabolites produced by actinomycete isolates from rhizospheric soil of mangrove forest of Torosiaje, Gorontalo, Indonesia, and identify the active compound for novel antibiotics production. Six isolates from a coastal mangrove forest was selected to produce secondary metabolite. The crude extract of the six selected actinomycete isolates showed antimicrobial activities against pathogenic microbes; the highest antimicrobial activities was indicated by metabolite produced by FUAm2-h1 and FMBg2-x3 isolates. The metabolite crude extracts produced by two potential isolates inhibited growth of pathogenic microbe on MIC value of 0.0625 to 0.5mgmL-1. Bio-autography assay detected an active compound on Rf value of 0.94, especially on extracellular metabolite produced by strain FUAm2-h1. The bioactive compounds were identified by liquid chromatography joined with low-resolution mass spectroscopy (LC/MS) and analysed by MEDINA's database The active compounds composed of 17 substances, and only 3 substances showed a high quantity with molecular weight of 507.37, 344.32 and 563.66 mol G-1, respectively. FTIR analyses identified the functional groups in the active compounds consisted of amide, amine, alkuna, alkane, NO2 nitro compound, alcohol, ether, carboxylic acid, ester and C-H aromatic ring. The biosynthesis of antibiotic on FUAm2-h1 and FMBg2-x3 isolates was regulated by double genes, i.e., PKS-II and NRPS genes. The antimicrobial activities of two actinomycete isolates showed the performance of antibiotics suspected as aromatics polyketides group. The FUAm2-h1 and FMBg2-x3 isolates showed high similarity with Streptomyces qinglanensis strain 172205 and Streptomyces sanyensis strain 219820, respectively, in terms of 16S rRNA gene sequences. The potential of those selected actinomycetes from extreme environments of mangrove forest constitute a source of promising actinomycete strains producing biologically active secondary metabolites.YULIANA RETNOWATISUKARTI MOELJOPAWIROTJUT SUGANDAWATY DJOHANENDANG SUTARININGSIH SOETARTOMBI & UNS Soloarticleactinomycetesantibioticsmangrove forestrhizospheric soilBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 2196-2203 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinomycetes antibiotics mangrove forest rhizospheric soil Biology (General) QH301-705.5
spellingShingle	actinomycetes antibiotics mangrove forest rhizospheric soil Biology (General) QH301-705.5 YULIANA RETNOWATI SUKARTI MOELJOPAWIRO TJUT SUGANDAWATY DJOHAN ENDANG SUTARININGSIH SOETARTO Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
description	Retnowati Y, Moeljopawiro S, Djohan TjS, Soetarto ES. 2018. Antimicrobial activities of actinomycetes isolates from rhizospheric soil on different mangrove forests of Torosiaje, Gorontalo, Indonesia. Biodiversitas 19: 2196-2203. Mangrove forests are very productive ecosystems that form unique saline environment very rich in organic matter, containing nitrogen and sulfur available for microorganisms. Mangrove forest as an extreme environment is promising to be sources of antibiotic-producing actinomycetes. The objectives of this study were to analyze the antimicrobial activities of metabolites produced by actinomycete isolates from rhizospheric soil of mangrove forest of Torosiaje, Gorontalo, Indonesia, and identify the active compound for novel antibiotics production. Six isolates from a coastal mangrove forest was selected to produce secondary metabolite. The crude extract of the six selected actinomycete isolates showed antimicrobial activities against pathogenic microbes; the highest antimicrobial activities was indicated by metabolite produced by FUAm2-h1 and FMBg2-x3 isolates. The metabolite crude extracts produced by two potential isolates inhibited growth of pathogenic microbe on MIC value of 0.0625 to 0.5mgmL-1. Bio-autography assay detected an active compound on Rf value of 0.94, especially on extracellular metabolite produced by strain FUAm2-h1. The bioactive compounds were identified by liquid chromatography joined with low-resolution mass spectroscopy (LC/MS) and analysed by MEDINA's database The active compounds composed of 17 substances, and only 3 substances showed a high quantity with molecular weight of 507.37, 344.32 and 563.66 mol G-1, respectively. FTIR analyses identified the functional groups in the active compounds consisted of amide, amine, alkuna, alkane, NO2 nitro compound, alcohol, ether, carboxylic acid, ester and C-H aromatic ring. The biosynthesis of antibiotic on FUAm2-h1 and FMBg2-x3 isolates was regulated by double genes, i.e., PKS-II and NRPS genes. The antimicrobial activities of two actinomycete isolates showed the performance of antibiotics suspected as aromatics polyketides group. The FUAm2-h1 and FMBg2-x3 isolates showed high similarity with Streptomyces qinglanensis strain 172205 and Streptomyces sanyensis strain 219820, respectively, in terms of 16S rRNA gene sequences. The potential of those selected actinomycetes from extreme environments of mangrove forest constitute a source of promising actinomycete strains producing biologically active secondary metabolites.
format	article
author	YULIANA RETNOWATI SUKARTI MOELJOPAWIRO TJUT SUGANDAWATY DJOHAN ENDANG SUTARININGSIH SOETARTO
author_facet	YULIANA RETNOWATI SUKARTI MOELJOPAWIRO TJUT SUGANDAWATY DJOHAN ENDANG SUTARININGSIH SOETARTO
author_sort	YULIANA RETNOWATI
title	Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
title_short	Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
title_full	Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
title_fullStr	Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
title_full_unstemmed	Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia
title_sort	antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of torosiaje, gorontalo, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/ca2ee0a67fb74e308ec7c7cb04edc0b7
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Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia

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