Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by Cercospora zeina

Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeina (C. zeina), is one of the most destructive soil-borne diseases in maize (Zea mays L.), and severely reduces maize production in Southwest China. However, the mechanism of res...

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Autores principales:	Wenzhu He, Yonghui Zhu, Yifeng Leng, Lin Yang, Biao Zhang, Junpin Yang, Xiao Zhang, Hai Lan, Haitao Tang, Jie Chen, Shibin Gao, Jun Tan, Jiwei Kang, Luchang Deng, Yan Li, Yuanyuan He, Tingzhao Rong, Moju Cao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	maize RNA-Seq gray leaf spot WGCNA disease resistance Botany QK1-989
Acceso en línea:	https://doaj.org/article/ca359c8ff65f4836ae89353ac13c84ef
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spelling	oai:doaj.org-article:ca359c8ff65f4836ae89353ac13c84ef2021-11-25T18:44:20ZTranscriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>10.3390/plants101122572223-7747https://doaj.org/article/ca359c8ff65f4836ae89353ac13c84ef2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2257https://doaj.org/toc/2223-7747Gray leaf spot (GLS), caused by the fungal pathogen <i>Cercospora zeina</i> (<i>C. zeina</i>), is one of the most destructive soil-borne diseases in maize (<i>Zea mays</i> L.), and severely reduces maize production in Southwest China. However, the mechanism of resistance to GLS is not clear and few resistant alleles have been identified. Two maize inbred lines, which were shown to be resistant (R6) and susceptible (S8) to GLS, were injected by <i>C. zeina</i> spore suspensions. Transcriptome analysis was carried out with leaf tissue at 0, 6, 24, 144, and 240 h after inoculation. Compared with 0 h of inoculation, a total of 667 and 419 stable common differentially expressed genes (DEGs) were found in the resistant and susceptible lines across the four timepoints, respectively. The DEGs were usually enriched in ‘response to stimulus’ and ‘response to stress’ in GO term analysis, and ‘plant–pathogen interaction’, ‘MAPK signaling pathways’, and ‘plant hormone signal transduction’ pathways, which were related to maize’s response to GLS, were enriched in KEGG analysis. Weighted-Genes Co-expression Network Analysis (WGCNA) identified two modules, while twenty hub genes identified from these indicated that plant hormone signaling, calcium signaling pathways, and transcription factors played a central role in GLS sensing and response. Combing DEGs and QTL mapping, five genes were identified as the consensus genes for the resistance of GLS. Two genes, were both putative Leucine-rich repeat protein kinase family proteins, specifically expressed in R6. In summary, our results can provide resources for gene mining and exploring the mechanism of resistance to GLS in maize.Wenzhu HeYonghui ZhuYifeng LengLin YangBiao ZhangJunpin YangXiao ZhangHai LanHaitao TangJie ChenShibin GaoJun TanJiwei KangLuchang DengYan LiYuanyuan HeTingzhao RongMoju CaoMDPI AGarticlemaizeRNA-Seqgray leaf spotWGCNAdisease resistanceBotanyQK1-989ENPlants, Vol 10, Iss 2257, p 2257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maize RNA-Seq gray leaf spot WGCNA disease resistance Botany QK1-989
spellingShingle	maize RNA-Seq gray leaf spot WGCNA disease resistance Botany QK1-989 Wenzhu He Yonghui Zhu Yifeng Leng Lin Yang Biao Zhang Junpin Yang Xiao Zhang Hai Lan Haitao Tang Jie Chen Shibin Gao Jun Tan Jiwei Kang Luchang Deng Yan Li Yuanyuan He Tingzhao Rong Moju Cao Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
description	Gray leaf spot (GLS), caused by the fungal pathogen <i>Cercospora zeina</i> (<i>C. zeina</i>), is one of the most destructive soil-borne diseases in maize (<i>Zea mays</i> L.), and severely reduces maize production in Southwest China. However, the mechanism of resistance to GLS is not clear and few resistant alleles have been identified. Two maize inbred lines, which were shown to be resistant (R6) and susceptible (S8) to GLS, were injected by <i>C. zeina</i> spore suspensions. Transcriptome analysis was carried out with leaf tissue at 0, 6, 24, 144, and 240 h after inoculation. Compared with 0 h of inoculation, a total of 667 and 419 stable common differentially expressed genes (DEGs) were found in the resistant and susceptible lines across the four timepoints, respectively. The DEGs were usually enriched in ‘response to stimulus’ and ‘response to stress’ in GO term analysis, and ‘plant–pathogen interaction’, ‘MAPK signaling pathways’, and ‘plant hormone signal transduction’ pathways, which were related to maize’s response to GLS, were enriched in KEGG analysis. Weighted-Genes Co-expression Network Analysis (WGCNA) identified two modules, while twenty hub genes identified from these indicated that plant hormone signaling, calcium signaling pathways, and transcription factors played a central role in GLS sensing and response. Combing DEGs and QTL mapping, five genes were identified as the consensus genes for the resistance of GLS. Two genes, were both putative Leucine-rich repeat protein kinase family proteins, specifically expressed in R6. In summary, our results can provide resources for gene mining and exploring the mechanism of resistance to GLS in maize.
format	article
author	Wenzhu He Yonghui Zhu Yifeng Leng Lin Yang Biao Zhang Junpin Yang Xiao Zhang Hai Lan Haitao Tang Jie Chen Shibin Gao Jun Tan Jiwei Kang Luchang Deng Yan Li Yuanyuan He Tingzhao Rong Moju Cao
author_facet	Wenzhu He Yonghui Zhu Yifeng Leng Lin Yang Biao Zhang Junpin Yang Xiao Zhang Hai Lan Haitao Tang Jie Chen Shibin Gao Jun Tan Jiwei Kang Luchang Deng Yan Li Yuanyuan He Tingzhao Rong Moju Cao
author_sort	Wenzhu He
title	Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
title_short	Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
title_full	Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
title_fullStr	Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
title_full_unstemmed	Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>
title_sort	transcriptomic analysis reveals candidate genes responding maize gray leaf spot caused by <i>cercospora zeina</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ca359c8ff65f4836ae89353ac13c84ef
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Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by <i>Cercospora zeina</i>

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