Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.

<h4>Background</h4>SARS-CoV-2 diagnosis relies on the performance of nasopharyngeal swabs. Alternative sample sites have been assessed but the heterogeneity of the studies have made comparing different sites difficult.<h4>Objectives</h4>Our aim was to compare the performance...

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Autores principales:	Dominik Kerimov, Pekka Tamminen, Hanna Viskari, Lauri Lehtimäki, Janne Aittoniemi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca396588ce17455380e79e74e69b2d1c
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spelling	oai:doaj.org-article:ca396588ce17455380e79e74e69b2d1c2021-12-02T20:16:20ZSampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.1932-620310.1371/journal.pone.0260184https://doaj.org/article/ca396588ce17455380e79e74e69b2d1c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0260184https://doaj.org/toc/1932-6203<h4>Background</h4>SARS-CoV-2 diagnosis relies on the performance of nasopharyngeal swabs. Alternative sample sites have been assessed but the heterogeneity of the studies have made comparing different sites difficult.<h4>Objectives</h4>Our aim was to compare the performance of four different sampling sites for SARS-CoV-2 samples with nasopharynx being the benchmark.<h4>Study design</h4>COVID-19 positive patients were recruited prospectively, and samples were collected and analysed for SARS-CoV-2 with RT-PCR from all four anatomical sites in 43 patients, who provided written informed consent.<h4>Results</h4>All anterior nasal and saliva samples were positive, while two oropharyngeal samples were negative. There was no significant difference in the cycle threshold values of nasopharyngeal and anterior nasal samples while saliva and oropharynx had higher cycle threshold values.<h4>Conclusions</h4>Anterior nasal swab performs as good as nasopharynx swab with saliva also finding all the positives but with higher cycle threshold values. Thus, we can conclude that anterior nasal swabs can be used for SARS-CoV-2 detection instead of nasopharyngeal swabs if the situation would require so.Dominik KerimovPekka TamminenHanna ViskariLauri LehtimäkiJanne AittoniemiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0260184 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominik Kerimov Pekka Tamminen Hanna Viskari Lauri Lehtimäki Janne Aittoniemi Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
description	<h4>Background</h4>SARS-CoV-2 diagnosis relies on the performance of nasopharyngeal swabs. Alternative sample sites have been assessed but the heterogeneity of the studies have made comparing different sites difficult.<h4>Objectives</h4>Our aim was to compare the performance of four different sampling sites for SARS-CoV-2 samples with nasopharynx being the benchmark.<h4>Study design</h4>COVID-19 positive patients were recruited prospectively, and samples were collected and analysed for SARS-CoV-2 with RT-PCR from all four anatomical sites in 43 patients, who provided written informed consent.<h4>Results</h4>All anterior nasal and saliva samples were positive, while two oropharyngeal samples were negative. There was no significant difference in the cycle threshold values of nasopharyngeal and anterior nasal samples while saliva and oropharynx had higher cycle threshold values.<h4>Conclusions</h4>Anterior nasal swab performs as good as nasopharynx swab with saliva also finding all the positives but with higher cycle threshold values. Thus, we can conclude that anterior nasal swabs can be used for SARS-CoV-2 detection instead of nasopharyngeal swabs if the situation would require so.
format	article
author	Dominik Kerimov Pekka Tamminen Hanna Viskari Lauri Lehtimäki Janne Aittoniemi
author_facet	Dominik Kerimov Pekka Tamminen Hanna Viskari Lauri Lehtimäki Janne Aittoniemi
author_sort	Dominik Kerimov
title	Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
title_short	Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
title_full	Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
title_fullStr	Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
title_full_unstemmed	Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.
title_sort	sampling site for sars-cov-2 rt-pcr-an intrapatient four-site comparison from tampere, finland.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ca396588ce17455380e79e74e69b2d1c
work_keys_str_mv	AT dominikkerimov samplingsiteforsarscov2rtpcranintrapatientfoursitecomparisonfromtamperefinland AT pekkatamminen samplingsiteforsarscov2rtpcranintrapatientfoursitecomparisonfromtamperefinland AT hannaviskari samplingsiteforsarscov2rtpcranintrapatientfoursitecomparisonfromtamperefinland AT laurilehtimaki samplingsiteforsarscov2rtpcranintrapatientfoursitecomparisonfromtamperefinland AT janneaittoniemi samplingsiteforsarscov2rtpcranintrapatientfoursitecomparisonfromtamperefinland
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Sampling site for SARS-CoV-2 RT-PCR-An intrapatient four-site comparison from Tampere, Finland.

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