Paracrine activity of adipose derived stem cells on limbal epithelial stem cells

Abstract Limbal stem cells deficiency (LSCD) is an eye disease caused by the loss of stem cells in the corneal limbus as a succession of an injury due physical, biological, or chemical agents. Current therapies of LSCD are focused on the transplantation of donor corneas or tissue equivalents produce...

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Autores principales:	Bartosz Sikora, Aleksandra Skubis-Sikora, Agnieszka Prusek, Joanna Gola
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca3cb924408a497f8a2d34433a256cb7
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spelling	oai:doaj.org-article:ca3cb924408a497f8a2d34433a256cb72021-12-02T18:01:40ZParacrine activity of adipose derived stem cells on limbal epithelial stem cells10.1038/s41598-021-99435-12045-2322https://doaj.org/article/ca3cb924408a497f8a2d34433a256cb72021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99435-1https://doaj.org/toc/2045-2322Abstract Limbal stem cells deficiency (LSCD) is an eye disease caused by the loss of stem cells in the corneal limbus as a succession of an injury due physical, biological, or chemical agents. Current therapies of LSCD are focused on the transplantation of donor corneas or tissue equivalents produced from autologous limbal stem cells. Every year there are waiting millions of patients for the cornea transplantation all over the world and the list is growing due to the relatively low number of cornea donors. On the other hand, the transplantation of tissue or cells into the recipient’s body is associated with the higher risk of possible side effects. The possibility of the application of an indirect treatment using the properties of the paracrine activity of stem cells, would be beneficial for the patients with transplant failures. This study was to evaluate the paracrine effect of mesenchymal stem cells derived from adipose tissue (ADSC) on the viability of limbal epithelial stem cells (LESC). The paracrine effect was assessed by treating LESC with conditioned medium collected from ADSC culture. Cell viability, cytotoxicity, apoptosis and proliferation were evaluated using in vitro assays in standard conditions and induced inflammation. After the exposure to the examined conditions, the expression of genes related to pro- and anti- inflammatory factors was evaluated and compared to the secretion of selected cytokines by ELISA test. Moreover, the changes in LESC phenotype were assessed using of phenotype microarrays. Our findings suggest that paracrine activity of ADSC on LESC promotes its proliferation and has a potential role in mitigation of the adverse impact of inflammation induced by lipopolysaccharide.Bartosz SikoraAleksandra Skubis-SikoraAgnieszka PrusekJoanna GolaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bartosz Sikora Aleksandra Skubis-Sikora Agnieszka Prusek Joanna Gola Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
description	Abstract Limbal stem cells deficiency (LSCD) is an eye disease caused by the loss of stem cells in the corneal limbus as a succession of an injury due physical, biological, or chemical agents. Current therapies of LSCD are focused on the transplantation of donor corneas or tissue equivalents produced from autologous limbal stem cells. Every year there are waiting millions of patients for the cornea transplantation all over the world and the list is growing due to the relatively low number of cornea donors. On the other hand, the transplantation of tissue or cells into the recipient’s body is associated with the higher risk of possible side effects. The possibility of the application of an indirect treatment using the properties of the paracrine activity of stem cells, would be beneficial for the patients with transplant failures. This study was to evaluate the paracrine effect of mesenchymal stem cells derived from adipose tissue (ADSC) on the viability of limbal epithelial stem cells (LESC). The paracrine effect was assessed by treating LESC with conditioned medium collected from ADSC culture. Cell viability, cytotoxicity, apoptosis and proliferation were evaluated using in vitro assays in standard conditions and induced inflammation. After the exposure to the examined conditions, the expression of genes related to pro- and anti- inflammatory factors was evaluated and compared to the secretion of selected cytokines by ELISA test. Moreover, the changes in LESC phenotype were assessed using of phenotype microarrays. Our findings suggest that paracrine activity of ADSC on LESC promotes its proliferation and has a potential role in mitigation of the adverse impact of inflammation induced by lipopolysaccharide.
format	article
author	Bartosz Sikora Aleksandra Skubis-Sikora Agnieszka Prusek Joanna Gola
author_facet	Bartosz Sikora Aleksandra Skubis-Sikora Agnieszka Prusek Joanna Gola
author_sort	Bartosz Sikora
title	Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
title_short	Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
title_full	Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
title_fullStr	Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
title_full_unstemmed	Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
title_sort	paracrine activity of adipose derived stem cells on limbal epithelial stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ca3cb924408a497f8a2d34433a256cb7
work_keys_str_mv	AT bartoszsikora paracrineactivityofadiposederivedstemcellsonlimbalepithelialstemcells AT aleksandraskubissikora paracrineactivityofadiposederivedstemcellsonlimbalepithelialstemcells AT agnieszkaprusek paracrineactivityofadiposederivedstemcellsonlimbalepithelialstemcells AT joannagola paracrineactivityofadiposederivedstemcellsonlimbalepithelialstemcells
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Paracrine activity of adipose derived stem cells on limbal epithelial stem cells

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