Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.

<h4>Background</h4>Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sour...

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Autor principal:	Jason L Rasgon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca3edfcff0c746e08de18d994c682cc8
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spelling	oai:doaj.org-article:ca3edfcff0c746e08de18d994c682cc82021-11-25T06:12:24ZStable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.1932-620310.1371/journal.pone.0002198https://doaj.org/article/ca3edfcff0c746e08de18d994c682cc82008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18493314/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sources are only useful for approximately 36 hours post-feeding. A critical need exists for technologies to extend this window and gain a more complete picture of mosquito feeding behavior for epidemiological studies. Stable isotopes are useful for investigating organism feeding behavior because the isotopic ratio of an organism's tissues reflects that of the material it ingests.<h4>Methodology/principal findings</h4>Proof-of-principle data indicates that after bloodfeeding, Aedes albopictus mosquitoes acquire diagnostic Carbon and Nitrogen stable isotope profiles from their vertebrate hosts that can be accurately identified one week post-feeding, approximately 4 days after the entire bloodmeal has been digested. Total C/N ratio served as a biomarker marker for bloodfeeding (P<0.02), while deltaN was the most informative variable which could distinguish between unfed, chicken-fed and human-fed mosquitoes (P<0.01). By plotting C/N vs. deltaN, all feeding treatments could be identified in a double-blind analysis.<h4>Conclusions/significance</h4>These proof-of-principle experiments indicate that analysis of stable isotopes can be used to distinguish bloodfed from unfed mosquitoes, and also distinguish between different vertebrate bloodmeal sources even after all blood has been digested. The development of stable isotope-based assays for mosquito bloodmeal identification may be a powerful tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens.Jason L RasgonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 5, p e2198 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jason L Rasgon Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
description	<h4>Background</h4>Vertebrate bloodfeeding is a critical component of a mosquito's ability to transmit pathogens that cause diseases such as malaria, dengue fever and viral encephalitis. Due to degradation by the digestive process, current methods to identify mosquito bloodmeal sources are only useful for approximately 36 hours post-feeding. A critical need exists for technologies to extend this window and gain a more complete picture of mosquito feeding behavior for epidemiological studies. Stable isotopes are useful for investigating organism feeding behavior because the isotopic ratio of an organism's tissues reflects that of the material it ingests.<h4>Methodology/principal findings</h4>Proof-of-principle data indicates that after bloodfeeding, Aedes albopictus mosquitoes acquire diagnostic Carbon and Nitrogen stable isotope profiles from their vertebrate hosts that can be accurately identified one week post-feeding, approximately 4 days after the entire bloodmeal has been digested. Total C/N ratio served as a biomarker marker for bloodfeeding (P<0.02), while deltaN was the most informative variable which could distinguish between unfed, chicken-fed and human-fed mosquitoes (P<0.01). By plotting C/N vs. deltaN, all feeding treatments could be identified in a double-blind analysis.<h4>Conclusions/significance</h4>These proof-of-principle experiments indicate that analysis of stable isotopes can be used to distinguish bloodfed from unfed mosquitoes, and also distinguish between different vertebrate bloodmeal sources even after all blood has been digested. The development of stable isotope-based assays for mosquito bloodmeal identification may be a powerful tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens.
format	article
author	Jason L Rasgon
author_facet	Jason L Rasgon
author_sort	Jason L Rasgon
title	Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
title_short	Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
title_full	Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
title_fullStr	Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
title_full_unstemmed	Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
title_sort	stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/ca3edfcff0c746e08de18d994c682cc8
work_keys_str_mv	AT jasonlrasgon stableisotopeanalysiscanpotentiallyidentifycompletelydigestedbloodmealsinmosquitoes
_version_	1718414069997764608

Stable isotope analysis can potentially identify completely-digested bloodmeals in mosquitoes.

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