The Denture-Associated Oral Microbiome in Health and Stomatitis

ABSTRACT While investigation of the microbiome on natural oral surfaces has generated a wealth of information, few studies have examined the microbial communities colonizing dentures and their relationship to oral health. To address this knowledge gap, we characterized the bacterial community associ...

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Autores principales:	Baochen Shi, Tingxi Wu, Jeffrey McLean, Anna Edlund, Youngik Young, Xuesong He, Hongyang Lv, Xuedong Zhou, Wenyuan Shi, Huiying Li, Renate Lux
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	bacteria denture microbiome stomatitis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ca49e9c42c9840f896862dd4f05cc193
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spelling	oai:doaj.org-article:ca49e9c42c9840f896862dd4f05cc1932021-11-15T15:22:03ZThe Denture-Associated Oral Microbiome in Health and Stomatitis10.1128/mSphere.00215-162379-5042https://doaj.org/article/ca49e9c42c9840f896862dd4f05cc1932016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00215-16https://doaj.org/toc/2379-5042ABSTRACT While investigation of the microbiome on natural oral surfaces has generated a wealth of information, few studies have examined the microbial communities colonizing dentures and their relationship to oral health. To address this knowledge gap, we characterized the bacterial community associated with dentures and remaining teeth in healthy individuals and patients with denture stomatitis. The microbiome compositions of matched denture and tooth plaque samples of 10 healthy individuals and 9 stomatitis patients were determined by 16S rRNA gene pyrosequencing. The microbial communities colonizing dentures and remaining teeth in health and disease were very similar to each other. Matched denture and tooth samples from the same individuals shared a significantly higher percentage of identical phylotypes than random pairs of samples from different study participants. Despite these overall similarities, several bacterial phylotypes displayed discrete health- and stomatitis-associated denture colonization, while others were distinct in health and disease independently of the surface. Certain phylotypes exhibited differential colonization of dentures and teeth independently of denture health status. In conclusion, denture and natural tooth surfaces in health and stomatitis harbor similar bacterial communities. Individual-related rather than surface-specific factors play a significant role in the bacterial phylotype composition colonizing dentures and teeth. This individual-specific mutual influence on denture and tooth surface colonization could be an important factor in maintaining oral health in denture wearers. Discrete differences in colonization patterns for distinct genera and phylotypes warrant further studies regarding their potential involvement or utility as specific indicators of health and disease development in denture-wearing individuals. IMPORTANCE Denture stomatitis is a prevalent inflammatory condition of the mucosal tissue in denture wearers that is triggered by microorganisms. While Candida has been extensively studied for its role in stomatitis etiology, the bacterial component largely remains to be investigated. Our data show that certain types of bacteria are significantly associated with denture health and disease. Furthermore, the bacterial communities residing on the teeth and dentures of the same person are similar to each other independently of the surface, and thus, denture health could impact the maintenance of remaining teeth and vice versa.Baochen ShiTingxi WuJeffrey McLeanAnna EdlundYoungik YoungXuesong HeHongyang LvXuedong ZhouWenyuan ShiHuiying LiRenate LuxAmerican Society for MicrobiologyarticlebacteriadenturemicrobiomestomatitisMicrobiologyQR1-502ENmSphere, Vol 1, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria denture microbiome stomatitis Microbiology QR1-502
spellingShingle	bacteria denture microbiome stomatitis Microbiology QR1-502 Baochen Shi Tingxi Wu Jeffrey McLean Anna Edlund Youngik Young Xuesong He Hongyang Lv Xuedong Zhou Wenyuan Shi Huiying Li Renate Lux The Denture-Associated Oral Microbiome in Health and Stomatitis
description	ABSTRACT While investigation of the microbiome on natural oral surfaces has generated a wealth of information, few studies have examined the microbial communities colonizing dentures and their relationship to oral health. To address this knowledge gap, we characterized the bacterial community associated with dentures and remaining teeth in healthy individuals and patients with denture stomatitis. The microbiome compositions of matched denture and tooth plaque samples of 10 healthy individuals and 9 stomatitis patients were determined by 16S rRNA gene pyrosequencing. The microbial communities colonizing dentures and remaining teeth in health and disease were very similar to each other. Matched denture and tooth samples from the same individuals shared a significantly higher percentage of identical phylotypes than random pairs of samples from different study participants. Despite these overall similarities, several bacterial phylotypes displayed discrete health- and stomatitis-associated denture colonization, while others were distinct in health and disease independently of the surface. Certain phylotypes exhibited differential colonization of dentures and teeth independently of denture health status. In conclusion, denture and natural tooth surfaces in health and stomatitis harbor similar bacterial communities. Individual-related rather than surface-specific factors play a significant role in the bacterial phylotype composition colonizing dentures and teeth. This individual-specific mutual influence on denture and tooth surface colonization could be an important factor in maintaining oral health in denture wearers. Discrete differences in colonization patterns for distinct genera and phylotypes warrant further studies regarding their potential involvement or utility as specific indicators of health and disease development in denture-wearing individuals. IMPORTANCE Denture stomatitis is a prevalent inflammatory condition of the mucosal tissue in denture wearers that is triggered by microorganisms. While Candida has been extensively studied for its role in stomatitis etiology, the bacterial component largely remains to be investigated. Our data show that certain types of bacteria are significantly associated with denture health and disease. Furthermore, the bacterial communities residing on the teeth and dentures of the same person are similar to each other independently of the surface, and thus, denture health could impact the maintenance of remaining teeth and vice versa.
format	article
author	Baochen Shi Tingxi Wu Jeffrey McLean Anna Edlund Youngik Young Xuesong He Hongyang Lv Xuedong Zhou Wenyuan Shi Huiying Li Renate Lux
author_facet	Baochen Shi Tingxi Wu Jeffrey McLean Anna Edlund Youngik Young Xuesong He Hongyang Lv Xuedong Zhou Wenyuan Shi Huiying Li Renate Lux
author_sort	Baochen Shi
title	The Denture-Associated Oral Microbiome in Health and Stomatitis
title_short	The Denture-Associated Oral Microbiome in Health and Stomatitis
title_full	The Denture-Associated Oral Microbiome in Health and Stomatitis
title_fullStr	The Denture-Associated Oral Microbiome in Health and Stomatitis
title_full_unstemmed	The Denture-Associated Oral Microbiome in Health and Stomatitis
title_sort	denture-associated oral microbiome in health and stomatitis
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/ca49e9c42c9840f896862dd4f05cc193
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The Denture-Associated Oral Microbiome in Health and Stomatitis

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