Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7

Yanzhao Bi, Yifan Zhang, Chunying Cui, Lulu Ren, Xueyun Jiang School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, People’s Republic of China Abstract: Nanodiamond (ND) is a renowned material in nonviral small interfering RNA (siRNA) carrier field...

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Autores principales:	Bi YZ, Zhang YF, Cui CY, Ren LL, Jiang XY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	NDCONH(CH2)2NH-VDGR siRNA carrier RNAi Gene silencing Survivin. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca4b5f7a6c504a24a654b863397f497f
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spelling	oai:doaj.org-article:ca4b5f7a6c504a24a654b863397f497f2021-12-02T08:07:34ZGene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-71178-2013https://doaj.org/article/ca4b5f7a6c504a24a654b863397f497f2016-11-01T00:00:00Zhttps://www.dovepress.com/gene-silencing-effects-of-anti-survivin-sirna-delivered-by-rgdv-functi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanzhao Bi, Yifan Zhang, Chunying Cui, Lulu Ren, Xueyun Jiang School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, People’s Republic of China Abstract: Nanodiamond (ND) is a renowned material in nonviral small interfering RNA (siRNA) carrier field due to its unique physical, chemical, and biological properties. In our previous work, it was proven that ND could deliver siRNA into cells efficiently and downregulate the expression of desired protein. However, synthesizing a high-efficient tumor-targeting carrier using ND is still a challenge. In this study, a novel carrier, NDCONH(CH2)2NH-VDGR, was synthesized for siRNA delivery, and its properties were characterized with methods including Fourier transform infrared spectrometry, transmission electron microscopy, scanning electron microscopy, gel retardation assay, differential scanning calorimetry, confocal microscopy, releasing test, real-time polymerase chain reaction (PCR) assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry, cytotoxicity assay, and gene-silencing efficacy assay in vitro and in vivo. The mechanism of NDCONH(CH2)2NH-VDGR/survivin-siRNA-induced tumor apoptosis was evaluated via flow cytometer assay using Annexin V–fluorescein isothiocyanate/propidium iodide staining method. The NDCONH(CH2)2NH-VDGR/survivin-siRNA nanoparticle with 60–110 nm diameter and 35.65±3.90 mV zeta potential was prepared. For real-time PCR assay, the results showed that the expression of survivin mRNA was reduced to 46.77%±6.3%. The expression of survivin protein was downregulated to 48.49%±2.25%, as evaluated by ELISA assay. MTT assay showed that NDCONH(CH2)2NH-VDGR/survivin-siRNA had an inhibitory effect on MCF-7 cell proliferation. According to these results, the survivin-siRNA could be delivered, transported, and released stably, which benefits in increasing the gene-silencing effect. Therefore, as an siRNA carrier, NDCONH(CH2)2NH-VDGR was suggested to be used in siRNA delivery system and in cancer treatments. Keywords: NDCONH(CH2)2NH-VDGR, siRNA carrier, RNAi, survivinBi YZZhang YFCui CYRen LLJiang XYDove Medical PressarticleNDCONH(CH2)2NH-VDGRsiRNA carrierRNAiGene silencingSurvivin.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5771-5787 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	NDCONH(CH2)2NH-VDGR siRNA carrier RNAi Gene silencing Survivin. Medicine (General) R5-920
spellingShingle	NDCONH(CH2)2NH-VDGR siRNA carrier RNAi Gene silencing Survivin. Medicine (General) R5-920 Bi YZ Zhang YF Cui CY Ren LL Jiang XY Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
description	Yanzhao Bi, Yifan Zhang, Chunying Cui, Lulu Ren, Xueyun Jiang School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, People’s Republic of China Abstract: Nanodiamond (ND) is a renowned material in nonviral small interfering RNA (siRNA) carrier field due to its unique physical, chemical, and biological properties. In our previous work, it was proven that ND could deliver siRNA into cells efficiently and downregulate the expression of desired protein. However, synthesizing a high-efficient tumor-targeting carrier using ND is still a challenge. In this study, a novel carrier, NDCONH(CH2)2NH-VDGR, was synthesized for siRNA delivery, and its properties were characterized with methods including Fourier transform infrared spectrometry, transmission electron microscopy, scanning electron microscopy, gel retardation assay, differential scanning calorimetry, confocal microscopy, releasing test, real-time polymerase chain reaction (PCR) assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry, cytotoxicity assay, and gene-silencing efficacy assay in vitro and in vivo. The mechanism of NDCONH(CH2)2NH-VDGR/survivin-siRNA-induced tumor apoptosis was evaluated via flow cytometer assay using Annexin V–fluorescein isothiocyanate/propidium iodide staining method. The NDCONH(CH2)2NH-VDGR/survivin-siRNA nanoparticle with 60–110 nm diameter and 35.65±3.90 mV zeta potential was prepared. For real-time PCR assay, the results showed that the expression of survivin mRNA was reduced to 46.77%±6.3%. The expression of survivin protein was downregulated to 48.49%±2.25%, as evaluated by ELISA assay. MTT assay showed that NDCONH(CH2)2NH-VDGR/survivin-siRNA had an inhibitory effect on MCF-7 cell proliferation. According to these results, the survivin-siRNA could be delivered, transported, and released stably, which benefits in increasing the gene-silencing effect. Therefore, as an siRNA carrier, NDCONH(CH2)2NH-VDGR was suggested to be used in siRNA delivery system and in cancer treatments. Keywords: NDCONH(CH2)2NH-VDGR, siRNA carrier, RNAi, survivin
format	article
author	Bi YZ Zhang YF Cui CY Ren LL Jiang XY
author_facet	Bi YZ Zhang YF Cui CY Ren LL Jiang XY
author_sort	Bi YZ
title	Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
title_short	Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
title_full	Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
title_fullStr	Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
title_full_unstemmed	Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
title_sort	gene-silencing effects of anti-survivin sirna delivered by rgdv-functionalized nanodiamond carrier in the breast carcinoma cell line mcf-7
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ca4b5f7a6c504a24a654b863397f497f
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Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7

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