A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L

Abstract Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytop...

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Autores principales:	Mei Ru, Kunru Wang, Zhenqing Bai, Liang Peng, Shaoxuan He, Yong Wang, Zongsuo Liang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca4bfdfb16a24219b1459cf83f20cc02
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spelling	oai:doaj.org-article:ca4bfdfb16a24219b1459cf83f20cc022021-12-02T15:04:55ZA tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L10.1038/s41598-017-05290-42045-2322https://doaj.org/article/ca4bfdfb16a24219b1459cf83f20cc022017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05290-4https://doaj.org/toc/2045-2322Abstract Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate. In vivo activity was confirmed by functional restoration of the Escherichia coli tyrosine auxotrophic mutant DL39. Agrobacterium rhizogenes-mediated antisense/sense expression of PvTAT in hairy roots was used to evaluate the contribution of PvTAT to RA synthesis. PvTAT were reduced by 46–95% and RA were decreased by 36–91% with low catalytic activity in antisense transgenic hairy root lines; furthermore, PvTAT were increased 0.77–2.6-fold with increased 1.3–1.8-fold RA and strong catalytic activity in sense transgenic hairy root lines compared with wild-type counterparts. The comprehensive physiological and catalytic evidence fills in the gap in RA-producing plants which didn’t provide evidence for TAT expression and catalytic activities in vitro and in vivo. That also highlights RA biosynthesis pathway in P. vulgaris and provides useful information to engineer natural products.Mei RuKunru WangZhenqing BaiLiang PengShaoxuan HeYong WangZongsuo LiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mei Ru Kunru Wang Zhenqing Bai Liang Peng Shaoxuan He Yong Wang Zongsuo Liang A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
description	Abstract Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate. In vivo activity was confirmed by functional restoration of the Escherichia coli tyrosine auxotrophic mutant DL39. Agrobacterium rhizogenes-mediated antisense/sense expression of PvTAT in hairy roots was used to evaluate the contribution of PvTAT to RA synthesis. PvTAT were reduced by 46–95% and RA were decreased by 36–91% with low catalytic activity in antisense transgenic hairy root lines; furthermore, PvTAT were increased 0.77–2.6-fold with increased 1.3–1.8-fold RA and strong catalytic activity in sense transgenic hairy root lines compared with wild-type counterparts. The comprehensive physiological and catalytic evidence fills in the gap in RA-producing plants which didn’t provide evidence for TAT expression and catalytic activities in vitro and in vivo. That also highlights RA biosynthesis pathway in P. vulgaris and provides useful information to engineer natural products.
format	article
author	Mei Ru Kunru Wang Zhenqing Bai Liang Peng Shaoxuan He Yong Wang Zongsuo Liang
author_facet	Mei Ru Kunru Wang Zhenqing Bai Liang Peng Shaoxuan He Yong Wang Zongsuo Liang
author_sort	Mei Ru
title	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
title_short	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
title_full	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
title_fullStr	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
title_full_unstemmed	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
title_sort	tyrosine aminotransferase involved in rosmarinic acid biosynthesis in prunella vulgaris l
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ca4bfdfb16a24219b1459cf83f20cc02
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A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L

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