Electrical activity in rat retina in a streptozotocin-induced diabetes model

Objectives: Diabetic retinopathy remains the major cause of blindness among the working-age population of developed countries. Considering this, experimental models of diabetes involving laboratory animals are important for assessing clinically significant methods to determine early pathologic alter...

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Autores principales:	Ekaterina M. Klochihina, Aleksey K. Erdyakov, Maria P. Morozova, Svetlana A. Gavrilova, Elena S. Akhapkina, Evgeniy V. Ivanov, Zera N. Dzhemilova, Ekaterina V. Artemova, Alla Y. Tokmakova, Vladimir B. Koshelev, Gagik R. Galstyan
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2018
Materias:	electroretinography ophthalmoscopy streptozotocin diabetic retinopathy Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/ca50da56e1f94e5c8d74a0574d86b2b8
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spelling	oai:doaj.org-article:ca50da56e1f94e5c8d74a0574d86b2b82021-11-14T09:00:22ZElectrical activity in rat retina in a streptozotocin-induced diabetes model2072-03512072-037810.14341/DM9490https://doaj.org/article/ca50da56e1f94e5c8d74a0574d86b2b82018-11-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/9490https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Objectives: Diabetic retinopathy remains the major cause of blindness among the working-age population of developed countries. Considering this, experimental models of diabetes involving laboratory animals are important for assessing clinically significant methods to determine early pathologic alterations of the retina. The early detection of diabetic retinopathy in combination with a search for new pathogenetic targets will enable focusing on new strategies to limit the development of critical changes in the retina and to prolong retinal functioning during the development of diabetes mellitus. Aim: This study aimed to define parameters of electroretinography test that identifies changes due to retinal impairment in diabetes. Methods: Experimental diabetes was induced in Wistar rats by intraperitoneally injecting streptozocin (65 mg/kg; group ‘DM’). The control group (‘CB’) received intraperitoneal injections of the vehicle, i.e. citric buffer. On each consecutive day of the experiment, all rats received insulin detemir (2 u/kg). Ophthalmoscopy and electroretinography were conducted before initiating the experiment and after 50, 58 and 66 days of injectin sptreptozocin. Results: Amid 2u\kg insulin injection the glucose level in venous blood in «DM» group amounted to 30-40 mM. The ophthalmoscopy showed that the optic nerve disk paled by the 50th day, with its line erasing. During electroretinography, wave amplitude in oscillatory potential test tended to decrease. β-wave latency of photopic system increased with α-wave latency of photopic system and α- and β-waves latency of scotopic system not altering. In addition, the amplitude of rhythmic stimulation of 8 and 12 Hz decreased. Conclusion: The most apparent parameters of electroretinography for modelling streptozocin-induced diabetes are wave amplitude during the oscillatory potential test, photopic B-wave latency and the amplitude of rhythmic stimulation. These results suggest that in diabetes, ischaemic injury is an important cause of early dysfunction of inner retinal layers.Ekaterina M. KlochihinaAleksey K. ErdyakovMaria P. MorozovaSvetlana A. GavrilovaElena S. AkhapkinaEvgeniy V. IvanovZera N. DzhemilovaEkaterina V. ArtemovaAlla Y. TokmakovaVladimir B. KoshelevGagik R. GalstyanEndocrinology Research Centrearticleelectroretinographyophthalmoscopystreptozotocindiabetic retinopathyNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 21, Iss 5, Pp 356-363 (2018)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	electroretinography ophthalmoscopy streptozotocin diabetic retinopathy Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	electroretinography ophthalmoscopy streptozotocin diabetic retinopathy Nutritional diseases. Deficiency diseases RC620-627 Ekaterina M. Klochihina Aleksey K. Erdyakov Maria P. Morozova Svetlana A. Gavrilova Elena S. Akhapkina Evgeniy V. Ivanov Zera N. Dzhemilova Ekaterina V. Artemova Alla Y. Tokmakova Vladimir B. Koshelev Gagik R. Galstyan Electrical activity in rat retina in a streptozotocin-induced diabetes model
description	Objectives: Diabetic retinopathy remains the major cause of blindness among the working-age population of developed countries. Considering this, experimental models of diabetes involving laboratory animals are important for assessing clinically significant methods to determine early pathologic alterations of the retina. The early detection of diabetic retinopathy in combination with a search for new pathogenetic targets will enable focusing on new strategies to limit the development of critical changes in the retina and to prolong retinal functioning during the development of diabetes mellitus. Aim: This study aimed to define parameters of electroretinography test that identifies changes due to retinal impairment in diabetes. Methods: Experimental diabetes was induced in Wistar rats by intraperitoneally injecting streptozocin (65 mg/kg; group ‘DM’). The control group (‘CB’) received intraperitoneal injections of the vehicle, i.e. citric buffer. On each consecutive day of the experiment, all rats received insulin detemir (2 u/kg). Ophthalmoscopy and electroretinography were conducted before initiating the experiment and after 50, 58 and 66 days of injectin sptreptozocin. Results: Amid 2u\kg insulin injection the glucose level in venous blood in «DM» group amounted to 30-40 mM. The ophthalmoscopy showed that the optic nerve disk paled by the 50th day, with its line erasing. During electroretinography, wave amplitude in oscillatory potential test tended to decrease. β-wave latency of photopic system increased with α-wave latency of photopic system and α- and β-waves latency of scotopic system not altering. In addition, the amplitude of rhythmic stimulation of 8 and 12 Hz decreased. Conclusion: The most apparent parameters of electroretinography for modelling streptozocin-induced diabetes are wave amplitude during the oscillatory potential test, photopic B-wave latency and the amplitude of rhythmic stimulation. These results suggest that in diabetes, ischaemic injury is an important cause of early dysfunction of inner retinal layers.
format	article
author	Ekaterina M. Klochihina Aleksey K. Erdyakov Maria P. Morozova Svetlana A. Gavrilova Elena S. Akhapkina Evgeniy V. Ivanov Zera N. Dzhemilova Ekaterina V. Artemova Alla Y. Tokmakova Vladimir B. Koshelev Gagik R. Galstyan
author_facet	Ekaterina M. Klochihina Aleksey K. Erdyakov Maria P. Morozova Svetlana A. Gavrilova Elena S. Akhapkina Evgeniy V. Ivanov Zera N. Dzhemilova Ekaterina V. Artemova Alla Y. Tokmakova Vladimir B. Koshelev Gagik R. Galstyan
author_sort	Ekaterina M. Klochihina
title	Electrical activity in rat retina in a streptozotocin-induced diabetes model
title_short	Electrical activity in rat retina in a streptozotocin-induced diabetes model
title_full	Electrical activity in rat retina in a streptozotocin-induced diabetes model
title_fullStr	Electrical activity in rat retina in a streptozotocin-induced diabetes model
title_full_unstemmed	Electrical activity in rat retina in a streptozotocin-induced diabetes model
title_sort	electrical activity in rat retina in a streptozotocin-induced diabetes model
publisher	Endocrinology Research Centre
publishDate	2018
url	https://doaj.org/article/ca50da56e1f94e5c8d74a0574d86b2b8
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Electrical activity in rat retina in a streptozotocin-induced diabetes model

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