Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation

Context Severe nephrotoxicity greatly limits the clinical use of the common effective chemotherapeutic agent cyclophosphamide (CYP). Huaiqihuang (HQH) is a Chinese herbal complex with various pharmacological activities, widely used for treating kidney disease. Objective This study estimates the prot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yueming Zhang, Jian Chang, Huan Gao, Xiaoyu Qu, Jinghui Zhai, Lina Tao, Jingmeng Sun, Yanqing Song
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	chinese medicine chemotherapeutic agent oxidative stress apoptosis inflammation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ca57ac7432d9427d8ee9d317979cfbc5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ca57ac7432d9427d8ee9d317979cfbc5
record_format	dspace
spelling	oai:doaj.org-article:ca57ac7432d9427d8ee9d317979cfbc52021-11-04T15:00:41ZHuaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation1388-02091744-511610.1080/13880209.2021.1990356https://doaj.org/article/ca57ac7432d9427d8ee9d317979cfbc52021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1990356https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Severe nephrotoxicity greatly limits the clinical use of the common effective chemotherapeutic agent cyclophosphamide (CYP). Huaiqihuang (HQH) is a Chinese herbal complex with various pharmacological activities, widely used for treating kidney disease. Objective This study estimates the protective effect of HQH against CYP-induced nephrotoxicity in rats. Materials and methods Four groups of 10 Sprague-Dawley rats were pre-treated with once-daily oral gavage of 3 and 6 mg/kg HQH for 5 days before receiving a single dose of CYP (200 mg/kg i.p.) on the 5th day; the control group received equivalent dose of saline. Renal function indices, morphological changes, oxidative stress, apoptosis and inflammatory mediators were measured. In addition, phosphorylation of the NF-κB/MAPK pathway and the activation of the NLRP3 inflammasome were analysed. Results Both doses of HQH reduced the levels of serum creatinine (31.27%, 43.61%), urea nitrogen (22.66%, 32.27%) and urine protein (12.87%, 15.98%) in the CYP-treated rats, and improved histopathological aberrations. Additionally, HQH decreased the production of MDA (37.02%, 46.18%) and increased the activities of antioxidant enzyme CAT (59.18%, 112.25%) and SOD (67.10%, 308.34%) after CYP treatment. HQH protected against CYP-induced nephrotoxicity by modulating apoptosis-related protein and suppressing the inflammatory responses. Furthermore, the phosphorylation of the NF-κB/MAPK pathway and the activation of the NLRP3 inflammasome were significantly boosted in CYP-treated rats, which was also abrogated by HQH treatment. Conclusions HQH effectively protected against CYP-induced nephrotoxicity, which was associated with regulating oxidative stress, apoptosis and inflammation, and so HQH may be a useful agent for treating nephrotoxicity caused by CYP.Yueming ZhangJian ChangHuan GaoXiaoyu QuJinghui ZhaiLina TaoJingmeng SunYanqing SongTaylor & Francis Grouparticlechinese medicinechemotherapeutic agentoxidative stressapoptosisinflammationTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1425-1431 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chinese medicine chemotherapeutic agent oxidative stress apoptosis inflammation Therapeutics. Pharmacology RM1-950
spellingShingle	chinese medicine chemotherapeutic agent oxidative stress apoptosis inflammation Therapeutics. Pharmacology RM1-950 Yueming Zhang Jian Chang Huan Gao Xiaoyu Qu Jinghui Zhai Lina Tao Jingmeng Sun Yanqing Song Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
description	Context Severe nephrotoxicity greatly limits the clinical use of the common effective chemotherapeutic agent cyclophosphamide (CYP). Huaiqihuang (HQH) is a Chinese herbal complex with various pharmacological activities, widely used for treating kidney disease. Objective This study estimates the protective effect of HQH against CYP-induced nephrotoxicity in rats. Materials and methods Four groups of 10 Sprague-Dawley rats were pre-treated with once-daily oral gavage of 3 and 6 mg/kg HQH for 5 days before receiving a single dose of CYP (200 mg/kg i.p.) on the 5th day; the control group received equivalent dose of saline. Renal function indices, morphological changes, oxidative stress, apoptosis and inflammatory mediators were measured. In addition, phosphorylation of the NF-κB/MAPK pathway and the activation of the NLRP3 inflammasome were analysed. Results Both doses of HQH reduced the levels of serum creatinine (31.27%, 43.61%), urea nitrogen (22.66%, 32.27%) and urine protein (12.87%, 15.98%) in the CYP-treated rats, and improved histopathological aberrations. Additionally, HQH decreased the production of MDA (37.02%, 46.18%) and increased the activities of antioxidant enzyme CAT (59.18%, 112.25%) and SOD (67.10%, 308.34%) after CYP treatment. HQH protected against CYP-induced nephrotoxicity by modulating apoptosis-related protein and suppressing the inflammatory responses. Furthermore, the phosphorylation of the NF-κB/MAPK pathway and the activation of the NLRP3 inflammasome were significantly boosted in CYP-treated rats, which was also abrogated by HQH treatment. Conclusions HQH effectively protected against CYP-induced nephrotoxicity, which was associated with regulating oxidative stress, apoptosis and inflammation, and so HQH may be a useful agent for treating nephrotoxicity caused by CYP.
format	article
author	Yueming Zhang Jian Chang Huan Gao Xiaoyu Qu Jinghui Zhai Lina Tao Jingmeng Sun Yanqing Song
author_facet	Yueming Zhang Jian Chang Huan Gao Xiaoyu Qu Jinghui Zhai Lina Tao Jingmeng Sun Yanqing Song
author_sort	Yueming Zhang
title	Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
title_short	Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
title_full	Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
title_fullStr	Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
title_full_unstemmed	Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation
title_sort	huaiqihuang (hqh) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the mapk/nf-κb pathway and nlrp3 inflammasome activation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ca57ac7432d9427d8ee9d317979cfbc5
work_keys_str_mv	AT yuemingzhang huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT jianchang huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT huangao huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT xiaoyuqu huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT jinghuizhai huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT linatao huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT jingmengsun huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation AT yanqingsong huaiqihuanghqhgranulealleviatescyclophosphamideinducednephrotoxicityviasuppressingthemapknfkbpathwayandnlrp3inflammasomeactivation
_version_	1718444798704091136

Huaiqihuang (HQH) granule alleviates cyclophosphamide-induced nephrotoxicity via suppressing the MAPK/NF-κB pathway and NLRP3 inflammasome activation

Ejemplares similares