Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.

The re-emergence of virulent strains of the Infectious Bursal Disease Virus (IBDV) leads to significant economic losses of poultry industry in Pakistan during last few years. This disease causes the infection of bursa, which leads to major immune losses. A total number of 30 samples from five IBD ou...

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Autores principales:	Sanaullah Sajid, Sajjad Ur Rahman, Mashkoor Mohsin Gilani, Zia Ud Din Sindhu, Manel Ben Ali, Amor Hedfi, Mohammed Almalki, Shahid Mahmood
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca58bd91f12a4560a29267368f13fe7c
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spelling	oai:doaj.org-article:ca58bd91f12a4560a29267368f13fe7c2021-12-02T20:18:06ZMolecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.1932-620310.1371/journal.pone.0254605https://doaj.org/article/ca58bd91f12a4560a29267368f13fe7c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254605https://doaj.org/toc/1932-6203The re-emergence of virulent strains of the Infectious Bursal Disease Virus (IBDV) leads to significant economic losses of poultry industry in Pakistan during last few years. This disease causes the infection of bursa, which leads to major immune losses. A total number of 30 samples from five IBD outbreaks during the period of 2019-20 were collected from different areas of Faisalabad district, Pakistan and assayed by targeting the IBD virus VP2 region through RT-PCR. Among all the outbreaks, almost 80% of poultry birds were found positive for the IBDV. The bursa tissues were collected from the infected birds and histopathological examination of samples revealed severe lymphocytic depletion, infiltration of inflammatory cells, and necrosis of the bursa of Fabricius (BF). Positive samples were subjected to re-isolation and molecular characterization of IBDV. The Pakistan IBDV genes were subjected to DNA sequencing to determine the virus nucleotide sequences. The sequences of 100 Serotype-I IBDVs showing nearest homology were compared and identified with the study sequence. The construction of the phylogenetic tree for nucleotide sequences was accomplished by the neighbor-joining method in MEGA-6 with reference strains. The VP2 segment reassortment of IBDVs carrying segment A were identified as one important type of circulating strains in Pakistan. The findings indicated the molecular features of the Pakistan IBDV strains playing a role in the evolution of new strains of the virus, which will contribute to the vaccine selection and effective prevention of the disease.Sanaullah SajidSajjad Ur RahmanMashkoor Mohsin GilaniZia Ud Din SindhuManel Ben AliAmor HedfiMohammed AlmalkiShahid MahmoodPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254605 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanaullah Sajid Sajjad Ur Rahman Mashkoor Mohsin Gilani Zia Ud Din Sindhu Manel Ben Ali Amor Hedfi Mohammed Almalki Shahid Mahmood Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
description	The re-emergence of virulent strains of the Infectious Bursal Disease Virus (IBDV) leads to significant economic losses of poultry industry in Pakistan during last few years. This disease causes the infection of bursa, which leads to major immune losses. A total number of 30 samples from five IBD outbreaks during the period of 2019-20 were collected from different areas of Faisalabad district, Pakistan and assayed by targeting the IBD virus VP2 region through RT-PCR. Among all the outbreaks, almost 80% of poultry birds were found positive for the IBDV. The bursa tissues were collected from the infected birds and histopathological examination of samples revealed severe lymphocytic depletion, infiltration of inflammatory cells, and necrosis of the bursa of Fabricius (BF). Positive samples were subjected to re-isolation and molecular characterization of IBDV. The Pakistan IBDV genes were subjected to DNA sequencing to determine the virus nucleotide sequences. The sequences of 100 Serotype-I IBDVs showing nearest homology were compared and identified with the study sequence. The construction of the phylogenetic tree for nucleotide sequences was accomplished by the neighbor-joining method in MEGA-6 with reference strains. The VP2 segment reassortment of IBDVs carrying segment A were identified as one important type of circulating strains in Pakistan. The findings indicated the molecular features of the Pakistan IBDV strains playing a role in the evolution of new strains of the virus, which will contribute to the vaccine selection and effective prevention of the disease.
format	article
author	Sanaullah Sajid Sajjad Ur Rahman Mashkoor Mohsin Gilani Zia Ud Din Sindhu Manel Ben Ali Amor Hedfi Mohammed Almalki Shahid Mahmood
author_facet	Sanaullah Sajid Sajjad Ur Rahman Mashkoor Mohsin Gilani Zia Ud Din Sindhu Manel Ben Ali Amor Hedfi Mohammed Almalki Shahid Mahmood
author_sort	Sanaullah Sajid
title	Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
title_short	Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
title_full	Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
title_fullStr	Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
title_full_unstemmed	Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.
title_sort	molecular characterization and demographic study on infectious bursal disease virus in faisalabad district.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ca58bd91f12a4560a29267368f13fe7c
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Molecular Characterization and Demographic Study on Infectious Bursal Disease Virus in Faisalabad District.

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