Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans

Laryssa Pinheiro Costa Silva,1 Jairo Pinto Oliveira,2 Wanderson Juvencio Keijok,2 André Romero da Silva,3 Anderson Rocha Aguiar,1 Marco Cesar Cunegundes Guimarães,2 Carolina Magri Ferraz,1 Jackson Victor Araújo,4 Fernando Luiz Tobias,5 Fábio Ribeiro Braga1...

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Autores principales:	Costa Silva LP, Oliveira JP, Keijok WJ, da Silva AR, Aguiar AR, Guimarães MCC, Ferraz CM, Araújo JV, Tobias FL, Braga FR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	silver nanoparticles green synthesis nematophagous fungi and Duddingtonia flagrans. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca5db764a16a4dd5aa1f1e50cbeec798
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spelling	oai:doaj.org-article:ca5db764a16a4dd5aa1f1e50cbeec7982021-12-02T02:28:07ZExtracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans1178-2013https://doaj.org/article/ca5db764a16a4dd5aa1f1e50cbeec7982017-08-01T00:00:00Zhttps://www.dovepress.com/extracellular-biosynthesis-of-silver-nanoparticles-using-the-cell-free-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Laryssa Pinheiro Costa Silva,1 Jairo Pinto Oliveira,2 Wanderson Juvencio Keijok,2 André Romero da Silva,3 Anderson Rocha Aguiar,1 Marco Cesar Cunegundes Guimarães,2 Carolina Magri Ferraz,1 Jackson Victor Araújo,4 Fernando Luiz Tobias,5 Fábio Ribeiro Braga1 1Department of Parasitology, University Vila Velha, Vila Velha, Espírito Santo, Brazil; 2Morphology Department, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; 3Federal Institute of Education, Science and Technology of Espírito Santo, Aracruz, Espírito Santo, Brazil; 4Department of Veterinary Medicine, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil; 5Department of Microbiology, University Vila Velha, Vila Velha, Espírito Santo, Brazil Abstract: The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion. Keywords: silver nanoparticles, green synthesis, nematophagous fungi, Duddingtonia flagransCosta Silva LPOliveira JPKeijok WJda Silva ARAguiar ARGuimarães MCCFerraz CMAraújo JVTobias FLBraga FRDove Medical Pressarticlesilver nanoparticlesgreen synthesisnematophagous fungi and Duddingtonia flagrans.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6373-6381 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles green synthesis nematophagous fungi and Duddingtonia flagrans. Medicine (General) R5-920
spellingShingle	silver nanoparticles green synthesis nematophagous fungi and Duddingtonia flagrans. Medicine (General) R5-920 Costa Silva LP Oliveira JP Keijok WJ da Silva AR Aguiar AR Guimarães MCC Ferraz CM Araújo JV Tobias FL Braga FR Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
description	Laryssa Pinheiro Costa Silva,1 Jairo Pinto Oliveira,2 Wanderson Juvencio Keijok,2 André Romero da Silva,3 Anderson Rocha Aguiar,1 Marco Cesar Cunegundes Guimarães,2 Carolina Magri Ferraz,1 Jackson Victor Araújo,4 Fernando Luiz Tobias,5 Fábio Ribeiro Braga1 1Department of Parasitology, University Vila Velha, Vila Velha, Espírito Santo, Brazil; 2Morphology Department, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; 3Federal Institute of Education, Science and Technology of Espírito Santo, Aracruz, Espírito Santo, Brazil; 4Department of Veterinary Medicine, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil; 5Department of Microbiology, University Vila Velha, Vila Velha, Espírito Santo, Brazil Abstract: The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion. Keywords: silver nanoparticles, green synthesis, nematophagous fungi, Duddingtonia flagrans
format	article
author	Costa Silva LP Oliveira JP Keijok WJ da Silva AR Aguiar AR Guimarães MCC Ferraz CM Araújo JV Tobias FL Braga FR
author_facet	Costa Silva LP Oliveira JP Keijok WJ da Silva AR Aguiar AR Guimarães MCC Ferraz CM Araújo JV Tobias FL Braga FR
author_sort	Costa Silva LP
title	Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
title_short	Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
title_full	Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
title_fullStr	Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
title_full_unstemmed	Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
title_sort	extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus duddingtonia flagrans
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/ca5db764a16a4dd5aa1f1e50cbeec798
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Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans

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