Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

Decellularization is the process of removing cellular components from native tissues or organs to obtain an acellular, collagenous scaffold for use in tissue engineering and organ regeneration. Surfactants are widely used to produce acellular scaffolds for clinical applications. However, cell–surfac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sonthikan Sitthisang, Jeeranan Boonruangkan, Meng Fatt Leong, Kerm Sin Chian, Young-Jin Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quantitative phase imaging L929 fibroblasts cell removal decellularization Applied optics. Photonics TA1501-1820
Acceso en línea:	https://doaj.org/article/ca66b16c660443cc93794d751d6ccc02
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ca66b16c660443cc93794d751d6ccc02
record_format	dspace
spelling	oai:doaj.org-article:ca66b16c660443cc93794d751d6ccc022021-11-25T18:43:33ZQuantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates10.3390/photonics81105082304-6732https://doaj.org/article/ca66b16c660443cc93794d751d6ccc022021-11-01T00:00:00Zhttps://www.mdpi.com/2304-6732/8/11/508https://doaj.org/toc/2304-6732Decellularization is the process of removing cellular components from native tissues or organs to obtain an acellular, collagenous scaffold for use in tissue engineering and organ regeneration. Surfactants are widely used to produce acellular scaffolds for clinical applications. However, cell–surfactants interactions have not been studied in depth. Cell-surfactant interaction was studied in a time-lapsed manner using sodium dodecyl sulfate (SDS) solution (surfactant) on adherent L929 fibroblasts as a test solution, phosphate-buffered saline (PBS) solution as control solution (isotonic), and deionized water as positive test solution (hypotonic), respectively. The QPI results show changes in the relative height and cross-sectional area of the cells, with various test solutions and exposure times. In particular, it was observed that the removal of the cell with SDS involved the disruption of the cellular membrane and detachment of the cell contents from the adhering surface. This study demonstrated the feasibility of using the QPI technique to understand the decellularization process.Sonthikan SitthisangJeeranan BoonruangkanMeng Fatt LeongKerm Sin ChianYoung-Jin KimMDPI AGarticlequantitative phase imagingL929 fibroblastscell removaldecellularizationApplied optics. PhotonicsTA1501-1820ENPhotonics, Vol 8, Iss 508, p 508 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantitative phase imaging L929 fibroblasts cell removal decellularization Applied optics. Photonics TA1501-1820
spellingShingle	quantitative phase imaging L929 fibroblasts cell removal decellularization Applied optics. Photonics TA1501-1820 Sonthikan Sitthisang Jeeranan Boonruangkan Meng Fatt Leong Kerm Sin Chian Young-Jin Kim Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
description	Decellularization is the process of removing cellular components from native tissues or organs to obtain an acellular, collagenous scaffold for use in tissue engineering and organ regeneration. Surfactants are widely used to produce acellular scaffolds for clinical applications. However, cell–surfactants interactions have not been studied in depth. Cell-surfactant interaction was studied in a time-lapsed manner using sodium dodecyl sulfate (SDS) solution (surfactant) on adherent L929 fibroblasts as a test solution, phosphate-buffered saline (PBS) solution as control solution (isotonic), and deionized water as positive test solution (hypotonic), respectively. The QPI results show changes in the relative height and cross-sectional area of the cells, with various test solutions and exposure times. In particular, it was observed that the removal of the cell with SDS involved the disruption of the cellular membrane and detachment of the cell contents from the adhering surface. This study demonstrated the feasibility of using the QPI technique to understand the decellularization process.
format	article
author	Sonthikan Sitthisang Jeeranan Boonruangkan Meng Fatt Leong Kerm Sin Chian Young-Jin Kim
author_facet	Sonthikan Sitthisang Jeeranan Boonruangkan Meng Fatt Leong Kerm Sin Chian Young-Jin Kim
author_sort	Sonthikan Sitthisang
title	Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
title_short	Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
title_full	Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
title_fullStr	Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
title_full_unstemmed	Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates
title_sort	quantitative phase imaging to study the effect of sodium dodecyl surfactant on adherent l929 fibroblasts on tissue culture plates
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ca66b16c660443cc93794d751d6ccc02
work_keys_str_mv	AT sonthikansitthisang quantitativephaseimagingtostudytheeffectofsodiumdodecylsurfactantonadherentl929fibroblastsontissuecultureplates AT jeerananboonruangkan quantitativephaseimagingtostudytheeffectofsodiumdodecylsurfactantonadherentl929fibroblastsontissuecultureplates AT mengfattleong quantitativephaseimagingtostudytheeffectofsodiumdodecylsurfactantonadherentl929fibroblastsontissuecultureplates AT kermsinchian quantitativephaseimagingtostudytheeffectofsodiumdodecylsurfactantonadherentl929fibroblastsontissuecultureplates AT youngjinkim quantitativephaseimagingtostudytheeffectofsodiumdodecylsurfactantonadherentl929fibroblastsontissuecultureplates
_version_	1718410799427354624

Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

Ejemplares similares