Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits

We have suggested that the molecular mechanism of vascular wall damage in diabetes is not substantially different from that in atherosclerosis. Thus, it can be assumed that aldehyde-modified LDL should be eliminated from the blood stream with much greater speed than non-oxidized LDL. In the availabl...

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Autores principales:	M. A. Grechnikova, S. P. Domogatskiy, G. G. Konovalova, A. K. Tikhaze, V. Z. Lankin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	oxidative stress modified ldl mda glyoxal methylglyoxal Science Q
Acceso en línea:	https://doaj.org/article/ca6b5b2f13c7409ca697c6893df434c5
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spelling	oai:doaj.org-article:ca6b5b2f13c7409ca697c6893df434c52021-11-23T06:14:34ZClearance of carbonyl-modified lipoproteins from the bloodstream of rabbits2541-94202587-959610.12737/article_590823a4b59f98.13160142https://doaj.org/article/ca6b5b2f13c7409ca697c6893df434c52016-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/198https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596We have suggested that the molecular mechanism of vascular wall damage in diabetes is not substantially different from that in atherosclerosis. Thus, it can be assumed that aldehyde-modified LDL should be eliminated from the blood stream with much greater speed than non-oxidized LDL. In the available literature there is information about the clearance of native human LDL from the bloodstream, whereas information on the clearance of the aldehyde-modified LDL in animals or humans was not found. Based on this, the present work is devoted to the clearance of aldehyde-modified LDL of rabbits and humans introduced into the bloodstream of rabbits. We investigated the clearance of glyoxal-, methylglyoxal- and MDA-modified LDL from the bloodstream of rabbits. We used biotinylated LDL of rabbit blood plasma and FITC-labeled LDL of human blood plasma. LDL was isolated with preparative ultracentrifugation in NaBr gradient. It was shown that glyoxal- and methylglyoxal-modified LDL of rabbits and humans circulated in the bloodstream for the same time as native LDL while MDA-modified LDL was rapidly eliminated from the bloodstream. The data obtained indicated the possibility of greater atherogenic potential of glyoxal- and methylglyoxal-modified LDL as they circulate in the bloodstream for a rather long time. At the same time, MDA-modified LDL is likely to be exposed to enhanced elimination by macrophages after their "linkage" to blood cells.M. A. GrechnikovaS. P. DomogatskiyG. G. KonovalovaA. K. TikhazeV. Z. LankinScientific Сentre for Family Health and Human Reproduction Problemsarticleoxidative stressmodified ldlmdaglyoxalmethylglyoxalScienceQRUActa Biomedica Scientifica, Vol 1, Iss 3(2), Pp 104-108 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	oxidative stress modified ldl mda glyoxal methylglyoxal Science Q
spellingShingle	oxidative stress modified ldl mda glyoxal methylglyoxal Science Q M. A. Grechnikova S. P. Domogatskiy G. G. Konovalova A. K. Tikhaze V. Z. Lankin Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
description	We have suggested that the molecular mechanism of vascular wall damage in diabetes is not substantially different from that in atherosclerosis. Thus, it can be assumed that aldehyde-modified LDL should be eliminated from the blood stream with much greater speed than non-oxidized LDL. In the available literature there is information about the clearance of native human LDL from the bloodstream, whereas information on the clearance of the aldehyde-modified LDL in animals or humans was not found. Based on this, the present work is devoted to the clearance of aldehyde-modified LDL of rabbits and humans introduced into the bloodstream of rabbits. We investigated the clearance of glyoxal-, methylglyoxal- and MDA-modified LDL from the bloodstream of rabbits. We used biotinylated LDL of rabbit blood plasma and FITC-labeled LDL of human blood plasma. LDL was isolated with preparative ultracentrifugation in NaBr gradient. It was shown that glyoxal- and methylglyoxal-modified LDL of rabbits and humans circulated in the bloodstream for the same time as native LDL while MDA-modified LDL was rapidly eliminated from the bloodstream. The data obtained indicated the possibility of greater atherogenic potential of glyoxal- and methylglyoxal-modified LDL as they circulate in the bloodstream for a rather long time. At the same time, MDA-modified LDL is likely to be exposed to enhanced elimination by macrophages after their "linkage" to blood cells.
format	article
author	M. A. Grechnikova S. P. Domogatskiy G. G. Konovalova A. K. Tikhaze V. Z. Lankin
author_facet	M. A. Grechnikova S. P. Domogatskiy G. G. Konovalova A. K. Tikhaze V. Z. Lankin
author_sort	M. A. Grechnikova
title	Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
title_short	Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
title_full	Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
title_fullStr	Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
title_full_unstemmed	Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
title_sort	clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/ca6b5b2f13c7409ca697c6893df434c5
work_keys_str_mv	AT magrechnikova clearanceofcarbonylmodifiedlipoproteinsfromthebloodstreamofrabbits AT spdomogatskiy clearanceofcarbonylmodifiedlipoproteinsfromthebloodstreamofrabbits AT ggkonovalova clearanceofcarbonylmodifiedlipoproteinsfromthebloodstreamofrabbits AT aktikhaze clearanceofcarbonylmodifiedlipoproteinsfromthebloodstreamofrabbits AT vzlankin clearanceofcarbonylmodifiedlipoproteinsfromthebloodstreamofrabbits
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Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits

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