Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

Abstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we h...

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Autores principales:	Saskia Seiser, Lukas Janker, Nina Zila, Michael Mildner, Ana Rakita, Johannes Matiasek, Andrea Bileck, Christopher Gerner, Verena Paulitschke, Adelheid Elbe-Bürger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca6e0d3da544492aad3a726a3e29cf09
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spelling	oai:doaj.org-article:ca6e0d3da544492aad3a726a3e29cf092021-12-02T11:46:07ZOctenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin10.1038/s41598-020-79378-92045-2322https://doaj.org/article/ca6e0d3da544492aad3a726a3e29cf092021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79378-9https://doaj.org/toc/2045-2322Abstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.Saskia SeiserLukas JankerNina ZilaMichael MildnerAna RakitaJohannes MatiasekAndrea BileckChristopher GernerVerena PaulitschkeAdelheid Elbe-BürgerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saskia Seiser Lukas Janker Nina Zila Michael Mildner Ana Rakita Johannes Matiasek Andrea Bileck Christopher Gerner Verena Paulitschke Adelheid Elbe-Bürger Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
description	Abstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.
format	article
author	Saskia Seiser Lukas Janker Nina Zila Michael Mildner Ana Rakita Johannes Matiasek Andrea Bileck Christopher Gerner Verena Paulitschke Adelheid Elbe-Bürger
author_facet	Saskia Seiser Lukas Janker Nina Zila Michael Mildner Ana Rakita Johannes Matiasek Andrea Bileck Christopher Gerner Verena Paulitschke Adelheid Elbe-Bürger
author_sort	Saskia Seiser
title	Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_short	Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_full	Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_fullStr	Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_full_unstemmed	Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_sort	octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ca6e0d3da544492aad3a726a3e29cf09
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Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

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