A capsid-encoded PPxY-motif facilitates adenovirus entry.

Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated...

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Autores principales:	Harald Wodrich, Daniel Henaff, Baptist Jammart, Carolina Segura-Morales, Sigrid Seelmeir, Olivier Coux, Zsolt Ruzsics, Christopher M Wiethoff, Eric J Kremer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ca71ac1e8ace4d4aa1b5123ec554c75a
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spelling	oai:doaj.org-article:ca71ac1e8ace4d4aa1b5123ec554c75a2021-11-25T05:48:14ZA capsid-encoded PPxY-motif facilitates adenovirus entry.1553-73661553-737410.1371/journal.ppat.1000808https://doaj.org/article/ca71ac1e8ace4d4aa1b5123ec554c75a2010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20333243/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens.Harald WodrichDaniel HenaffBaptist JammartCarolina Segura-MoralesSigrid SeelmeirOlivier CouxZsolt RuzsicsChristopher M WiethoffEric J KremerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 3, p e1000808 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer A capsid-encoded PPxY-motif facilitates adenovirus entry.
description	Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens.
format	article
author	Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer
author_facet	Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer
author_sort	Harald Wodrich
title	A capsid-encoded PPxY-motif facilitates adenovirus entry.
title_short	A capsid-encoded PPxY-motif facilitates adenovirus entry.
title_full	A capsid-encoded PPxY-motif facilitates adenovirus entry.
title_fullStr	A capsid-encoded PPxY-motif facilitates adenovirus entry.
title_full_unstemmed	A capsid-encoded PPxY-motif facilitates adenovirus entry.
title_sort	capsid-encoded ppxy-motif facilitates adenovirus entry.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ca71ac1e8ace4d4aa1b5123ec554c75a
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A capsid-encoded PPxY-motif facilitates adenovirus entry.

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