W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota

Abstract W27 monoclonal immunoglobulin A (IgA) suppresses pathogenic Escherichia coli cell growth; however, its effect on the human intestine remains unclear. We aimed to determine how W27 IgA affects the human colonic microbiota using the in vitro microbiota model. This model was established using...

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Autores principales:	Kengo Sasaki, Tomoyuki Mori, Namiko Hoshi, Daisuke Sasaki, Jun Inoue, Reiko Shinkura, Akihiko Kondo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca7297d40e694972942b781c4ed744e1
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spelling	oai:doaj.org-article:ca7297d40e694972942b781c4ed744e12021-12-02T16:08:06ZW27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota10.1038/s41598-021-94210-82045-2322https://doaj.org/article/ca7297d40e694972942b781c4ed744e12021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94210-8https://doaj.org/toc/2045-2322Abstract W27 monoclonal immunoglobulin A (IgA) suppresses pathogenic Escherichia coli cell growth; however, its effect on the human intestine remains unclear. We aimed to determine how W27 IgA affects the human colonic microbiota using the in vitro microbiota model. This model was established using fecal samples collected from 12 healthy volunteers; after anaerobic cultivation, each model was found to retain the genera found in the original human fecal samples. After pre-incubating W27 IgA with the respective fecal sample under aerobic conditions, the mixture of W27 IgA (final concentration, 0.5 μg/mL) and each fecal sample was added to the in vitro microbiota model and cultured under anaerobic conditions. Next-generation sequencing of the bacterial 16S rRNA gene revealed that W27 IgA significantly decreased the relative abundance of bacteria related to the genus Escherichia in the model. Additionally, at a final concentration of 5 μg/mL, W27 IgA delayed growth in the pure culture of Escherichia coli isolated from human fecal samples. Our study thus revealed the suppressive effect of W27 IgA on the genus Escherichia at relatively low-concentrations and the usefulness of an in vitro microbiota model to evaluate the effect of IgA as a gut microbiota regulator.Kengo SasakiTomoyuki MoriNamiko HoshiDaisuke SasakiJun InoueReiko ShinkuraAkihiko KondoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kengo Sasaki Tomoyuki Mori Namiko Hoshi Daisuke Sasaki Jun Inoue Reiko Shinkura Akihiko Kondo W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
description	Abstract W27 monoclonal immunoglobulin A (IgA) suppresses pathogenic Escherichia coli cell growth; however, its effect on the human intestine remains unclear. We aimed to determine how W27 IgA affects the human colonic microbiota using the in vitro microbiota model. This model was established using fecal samples collected from 12 healthy volunteers; after anaerobic cultivation, each model was found to retain the genera found in the original human fecal samples. After pre-incubating W27 IgA with the respective fecal sample under aerobic conditions, the mixture of W27 IgA (final concentration, 0.5 μg/mL) and each fecal sample was added to the in vitro microbiota model and cultured under anaerobic conditions. Next-generation sequencing of the bacterial 16S rRNA gene revealed that W27 IgA significantly decreased the relative abundance of bacteria related to the genus Escherichia in the model. Additionally, at a final concentration of 5 μg/mL, W27 IgA delayed growth in the pure culture of Escherichia coli isolated from human fecal samples. Our study thus revealed the suppressive effect of W27 IgA on the genus Escherichia at relatively low-concentrations and the usefulness of an in vitro microbiota model to evaluate the effect of IgA as a gut microbiota regulator.
format	article
author	Kengo Sasaki Tomoyuki Mori Namiko Hoshi Daisuke Sasaki Jun Inoue Reiko Shinkura Akihiko Kondo
author_facet	Kengo Sasaki Tomoyuki Mori Namiko Hoshi Daisuke Sasaki Jun Inoue Reiko Shinkura Akihiko Kondo
author_sort	Kengo Sasaki
title	W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
title_short	W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
title_full	W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
title_fullStr	W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
title_full_unstemmed	W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota
title_sort	w27 iga suppresses growth of escherichia in an in vitro model of the human intestinal microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ca7297d40e694972942b781c4ed744e1
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W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota

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