Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei

BACKGROUND AND OBJECTIVE: With regard to the drug resistance, special attention has been placed on researches for discovering new antimicrobial substances such as nanoparticles. In green synthesis biological resources such as bacteria and yeast are used for producing metal nanoparticles. In this re...

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Autores principales:	P Torabian, F Ghandehari, M Fatemi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	iron oxide nanoparticles lactobacillus casei staphylococcus aureus Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ca7b412bcb2a47f1a62e90c52aaf5fcd
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spelling	oai:doaj.org-article:ca7b412bcb2a47f1a62e90c52aaf5fcd2021-11-10T08:30:36ZEvaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei1561-41072251-7170https://doaj.org/article/ca7b412bcb2a47f1a62e90c52aaf5fcd2019-12-01T00:00:00Zhttp://jbums.org/article-1-7813-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: With regard to the drug resistance, special attention has been placed on researches for discovering new antimicrobial substances such as nanoparticles. In green synthesis biological resources such as bacteria and yeast are used for producing metal nanoparticles. In this research, we used the Lactobacillus casei extract as a biological source for producing iron oxide nanoparticles and antibacterial effects of these nanoparticles against the standard strains of Escherichia coli and Staphylococcus aureus were investigated. METHODS: for synthesis of green iron oxide nanoparticles, cytoplasmic extract of Lactobacillus casei and iron sulfate solution 10-3M were mixed and incubated for 3 weeks at 37 ° C in the presence of 5% carbon dioxide. Synthesis of iron oxide nanoparticles were evaluated using X-ray diffraction pattern and Transmission electron microscopy. Antibacterial effects of nanoparticles with dilution of 10, 100 and 1000 micro-grams per milliliter on two standard strains of Escherichia coli and Staphylococcus aureus were evaluated. FINDINGS: Synthesis of iron oxide crystals was confirmed by XRD analysis. Based on transmission electron microscopy the average of nanoparticles was about 15 nm with a spherical shape. Antimicrobial effect of nanoparticles showed that at concentration of 100 and 1000 µg/ml has an inhibitory effect on the growth on Staphylococcus aureus equal to 12.03±0.32 and 16±0.5, respectively. The only concentration that showed significant effect was 1000µg/ml (p≤0.001). While are ineffective against Escherichia coli. CONCLUSION: The results of this study indicated that producing iron oxide nanoparticles using cytoplasmic extract of Lactobacillus casei is a biologically safe method and very noteworthy in medicine and pharmacology and may be considered as a good candidate for the treatment of bacterial infections.P TorabianF GhandehariM FatemiBabol University of Medical Sciencesarticleiron oxide nanoparticleslactobacillus caseistaphylococcus aureusMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 237-241 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	iron oxide nanoparticles lactobacillus casei staphylococcus aureus Medicine R Medicine (General) R5-920
spellingShingle	iron oxide nanoparticles lactobacillus casei staphylococcus aureus Medicine R Medicine (General) R5-920 P Torabian F Ghandehari M Fatemi Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
description	BACKGROUND AND OBJECTIVE: With regard to the drug resistance, special attention has been placed on researches for discovering new antimicrobial substances such as nanoparticles. In green synthesis biological resources such as bacteria and yeast are used for producing metal nanoparticles. In this research, we used the Lactobacillus casei extract as a biological source for producing iron oxide nanoparticles and antibacterial effects of these nanoparticles against the standard strains of Escherichia coli and Staphylococcus aureus were investigated. METHODS: for synthesis of green iron oxide nanoparticles, cytoplasmic extract of Lactobacillus casei and iron sulfate solution 10-3M were mixed and incubated for 3 weeks at 37 ° C in the presence of 5% carbon dioxide. Synthesis of iron oxide nanoparticles were evaluated using X-ray diffraction pattern and Transmission electron microscopy. Antibacterial effects of nanoparticles with dilution of 10, 100 and 1000 micro-grams per milliliter on two standard strains of Escherichia coli and Staphylococcus aureus were evaluated. FINDINGS: Synthesis of iron oxide crystals was confirmed by XRD analysis. Based on transmission electron microscopy the average of nanoparticles was about 15 nm with a spherical shape. Antimicrobial effect of nanoparticles showed that at concentration of 100 and 1000 µg/ml has an inhibitory effect on the growth on Staphylococcus aureus equal to 12.03±0.32 and 16±0.5, respectively. The only concentration that showed significant effect was 1000µg/ml (p≤0.001). While are ineffective against Escherichia coli. CONCLUSION: The results of this study indicated that producing iron oxide nanoparticles using cytoplasmic extract of Lactobacillus casei is a biologically safe method and very noteworthy in medicine and pharmacology and may be considered as a good candidate for the treatment of bacterial infections.
format	article
author	P Torabian F Ghandehari M Fatemi
author_facet	P Torabian F Ghandehari M Fatemi
author_sort	P Torabian
title	Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
title_short	Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
title_full	Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
title_fullStr	Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
title_full_unstemmed	Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei
title_sort	evaluating antibacterial effect of green synthesis oxide iron nanoparticles using cytoplasmic extract of lactobacillus casei
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/ca7b412bcb2a47f1a62e90c52aaf5fcd
work_keys_str_mv	AT ptorabian evaluatingantibacterialeffectofgreensynthesisoxideironnanoparticlesusingcytoplasmicextractoflactobacilluscasei AT fghandehari evaluatingantibacterialeffectofgreensynthesisoxideironnanoparticlesusingcytoplasmicextractoflactobacilluscasei AT mfatemi evaluatingantibacterialeffectofgreensynthesisoxideironnanoparticlesusingcytoplasmicextractoflactobacilluscasei
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Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei

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