Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar

Abstract The Na+/H+ antiporters (NHXs) are secondary ion transporters to exchange H+ and transfer the Na+ or K+ across membrane, they play crucial roles during plant development and stress responses. To gain insight into the functional divergence of NHX genes in poplar, eight PtNHX were identified f...

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Autores principales:	Fengxia Tian, Ermei Chang, Yu Li, Pei Sun, Jianjun Hu, Jin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca822673aa364f77a3acf39d2cc20bb6
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spelling	oai:doaj.org-article:ca822673aa364f77a3acf39d2cc20bb62021-12-02T11:41:09ZExpression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar10.1038/s41598-017-02894-82045-2322https://doaj.org/article/ca822673aa364f77a3acf39d2cc20bb62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02894-8https://doaj.org/toc/2045-2322Abstract The Na+/H+ antiporters (NHXs) are secondary ion transporters to exchange H+ and transfer the Na+ or K+ across membrane, they play crucial roles during plant development and stress responses. To gain insight into the functional divergence of NHX genes in poplar, eight PtNHX were identified from Populus trichocarpa genome. PtNHXs containing 10 transmembrane helices (TMH) and a hydrophilic C-terminal domain, the TMH compose a hollow cylinder to provide the channel for Na+ and H+ transport. The expression patterns and cis-acting elements showed that all the PtNHXs were response to single or multiple stresses including drought, heat, cold, salinity, MV, and ABA. Both the co-expression network and protein-protein interaction network of PtNHXs implying their functional divergence. Interestingly, although PtNHX7 and PtNHX8 were generated by whole genome duplication event, they showed significant differences in expression pattern, protein structure, co-expressed genes, and interacted proteins. Only PtNHX7 interact with CBL and CIPK, indicating PtNHX7 is the primary NHX involved in CBL-CIPK pathway during salt stress responses. Natural variation analysis based on 549 P. trichocarpa individuals indicated the frequency of SNPs in PtNHX7 was significantly higher than other PtNHXs. Our findings provide new insights into the functional divergence of NHX genes in poplar.Fengxia TianErmei ChangYu LiPei SunJianjun HuJin ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fengxia Tian Ermei Chang Yu Li Pei Sun Jianjun Hu Jin Zhang Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
description	Abstract The Na+/H+ antiporters (NHXs) are secondary ion transporters to exchange H+ and transfer the Na+ or K+ across membrane, they play crucial roles during plant development and stress responses. To gain insight into the functional divergence of NHX genes in poplar, eight PtNHX were identified from Populus trichocarpa genome. PtNHXs containing 10 transmembrane helices (TMH) and a hydrophilic C-terminal domain, the TMH compose a hollow cylinder to provide the channel for Na+ and H+ transport. The expression patterns and cis-acting elements showed that all the PtNHXs were response to single or multiple stresses including drought, heat, cold, salinity, MV, and ABA. Both the co-expression network and protein-protein interaction network of PtNHXs implying their functional divergence. Interestingly, although PtNHX7 and PtNHX8 were generated by whole genome duplication event, they showed significant differences in expression pattern, protein structure, co-expressed genes, and interacted proteins. Only PtNHX7 interact with CBL and CIPK, indicating PtNHX7 is the primary NHX involved in CBL-CIPK pathway during salt stress responses. Natural variation analysis based on 549 P. trichocarpa individuals indicated the frequency of SNPs in PtNHX7 was significantly higher than other PtNHXs. Our findings provide new insights into the functional divergence of NHX genes in poplar.
format	article
author	Fengxia Tian Ermei Chang Yu Li Pei Sun Jianjun Hu Jin Zhang
author_facet	Fengxia Tian Ermei Chang Yu Li Pei Sun Jianjun Hu Jin Zhang
author_sort	Fengxia Tian
title	Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
title_short	Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
title_full	Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
title_fullStr	Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
title_full_unstemmed	Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar
title_sort	expression and integrated network analyses revealed functional divergence of nhx-type na+/h+ exchanger genes in poplar
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ca822673aa364f77a3acf39d2cc20bb6
work_keys_str_mv	AT fengxiatian expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar AT ermeichang expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar AT yuli expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar AT peisun expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar AT jianjunhu expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar AT jinzhang expressionandintegratednetworkanalysesrevealedfunctionaldivergenceofnhxtypenahexchangergenesinpoplar
_version_	1718395485144743936

Expression and integrated network analyses revealed functional divergence of NHX-type Na+/H+ exchanger genes in poplar

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