Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
The aim of this study was to investigate the presence of iron-uptake and virulence genes, antibiotic resistance profiles, and phylogenetic relatedness in 115 <i>Escherichia coli</i> (<i>E. coli</i>) strains isolated from broilers in Slovakia and to determine their potential t...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/ca838db77ed4411b9aff65235ffa1bb1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | The aim of this study was to investigate the presence of iron-uptake and virulence genes, antibiotic resistance profiles, and phylogenetic relatedness in 115 <i>Escherichia coli</i> (<i>E. coli</i>) strains isolated from broilers in Slovakia and to determine their potential threat to human health. The most frequent phylogroups were B1 (37%) and A (21%), and 33.9% strains were included in pathogenic groups. The commonly observed iron-uptake genes were <i>feoB</i> (94%), <i>sitA</i> (83%), and <i>iutA</i> (58%). Protectins (<i>iss, kpsMTII</i>) were identified in 30% of samples. Four percent of B2-associated broilers carried the <i>papC</i> (P fimbria) gene connected with upper urinary tract infection. The dominant resistance was to tetracycline (49%), ampicillin (66%), ampicillin + sulbactam (27%), ciprofloxacin (61%), and trimethoprim + sulfonamide (34%); moreover, sporadically occurring resistance to cephalosporins, aminoglycosides, fluoroquinolones, and polypeptide colistin was observed. Genotypic analysis of resistance revealed the presence of <i>bla<sub>CTX-M-1</sub></i> and <i>bla<sub>CTX-M-2</sub></i> in two isolates from broilers. Commercial broilers can be reservoirs of virulent and resistant genes as well as <i>E. coli</i> causing (extra-)intestinal infections, which can be a potential threat to humans via direct contact and food. |
|---|