Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers

The aim of this study was to investigate the presence of iron-uptake and virulence genes, antibiotic resistance profiles, and phylogenetic relatedness in 115 Escherichia coli (E. coli) strains isolated from broilers in Slovakia and to determine their potential t...

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Autores principales:	Tímea Kocúreková, Lívia Karahutová, Dobroslava Bujňáková
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>E. coli</i> broilers iron uptake phylogenetic groups virulence-associated genes antimicrobial resistance Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ca838db77ed4411b9aff65235ffa1bb1
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spelling	oai:doaj.org-article:ca838db77ed4411b9aff65235ffa1bb12021-11-25T16:22:24ZAntimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers10.3390/antibiotics101113032079-6382https://doaj.org/article/ca838db77ed4411b9aff65235ffa1bb12021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1303https://doaj.org/toc/2079-6382The aim of this study was to investigate the presence of iron-uptake and virulence genes, antibiotic resistance profiles, and phylogenetic relatedness in 115 <i>Escherichia coli</i> (<i>E. coli</i>) strains isolated from broilers in Slovakia and to determine their potential threat to human health. The most frequent phylogroups were B1 (37%) and A (21%), and 33.9% strains were included in pathogenic groups. The commonly observed iron-uptake genes were <i>feoB</i> (94%), <i>sitA</i> (83%), and <i>iutA</i> (58%). Protectins (<i>iss, kpsMTII</i>) were identified in 30% of samples. Four percent of B2-associated broilers carried the <i>papC</i> (P fimbria) gene connected with upper urinary tract infection. The dominant resistance was to tetracycline (49%), ampicillin (66%), ampicillin + sulbactam (27%), ciprofloxacin (61%), and trimethoprim + sulfonamide (34%); moreover, sporadically occurring resistance to cephalosporins, aminoglycosides, fluoroquinolones, and polypeptide colistin was observed. Genotypic analysis of resistance revealed the presence of <i>bla<sub>CTX-M-1</sub></i> and <i>bla<sub>CTX-M-2</sub></i> in two isolates from broilers. Commercial broilers can be reservoirs of virulent and resistant genes as well as <i>E. coli</i> causing (extra-)intestinal infections, which can be a potential threat to humans via direct contact and food.Tímea KocúrekováLívia KarahutováDobroslava BujňákováMDPI AGarticle<i>E. coli</i>broilersiron uptakephylogenetic groupsvirulence-associated genesantimicrobial resistanceTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1303, p 1303 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>E. coli</i> broilers iron uptake phylogenetic groups virulence-associated genes antimicrobial resistance Therapeutics. Pharmacology RM1-950
spellingShingle	<i>E. coli</i> broilers iron uptake phylogenetic groups virulence-associated genes antimicrobial resistance Therapeutics. Pharmacology RM1-950 Tímea Kocúreková Lívia Karahutová Dobroslava Bujňáková Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
description	The aim of this study was to investigate the presence of iron-uptake and virulence genes, antibiotic resistance profiles, and phylogenetic relatedness in 115 <i>Escherichia coli</i> (<i>E. coli</i>) strains isolated from broilers in Slovakia and to determine their potential threat to human health. The most frequent phylogroups were B1 (37%) and A (21%), and 33.9% strains were included in pathogenic groups. The commonly observed iron-uptake genes were <i>feoB</i> (94%), <i>sitA</i> (83%), and <i>iutA</i> (58%). Protectins (<i>iss, kpsMTII</i>) were identified in 30% of samples. Four percent of B2-associated broilers carried the <i>papC</i> (P fimbria) gene connected with upper urinary tract infection. The dominant resistance was to tetracycline (49%), ampicillin (66%), ampicillin + sulbactam (27%), ciprofloxacin (61%), and trimethoprim + sulfonamide (34%); moreover, sporadically occurring resistance to cephalosporins, aminoglycosides, fluoroquinolones, and polypeptide colistin was observed. Genotypic analysis of resistance revealed the presence of <i>bla<sub>CTX-M-1</sub></i> and <i>bla<sub>CTX-M-2</sub></i> in two isolates from broilers. Commercial broilers can be reservoirs of virulent and resistant genes as well as <i>E. coli</i> causing (extra-)intestinal infections, which can be a potential threat to humans via direct contact and food.
format	article
author	Tímea Kocúreková Lívia Karahutová Dobroslava Bujňáková
author_facet	Tímea Kocúreková Lívia Karahutová Dobroslava Bujňáková
author_sort	Tímea Kocúreková
title	Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
title_short	Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
title_full	Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
title_fullStr	Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
title_full_unstemmed	Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers
title_sort	antimicrobial susceptibility and detection of virulence-associated genes in <i>escherichia coli</i> strains isolated from commercial broilers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ca838db77ed4411b9aff65235ffa1bb1
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Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in <i>Escherichia coli</i> Strains Isolated from Commercial Broilers

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