Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

Furfural is a major inhibitor in lignocellulose hydrolysate for Zymomonas mobilis. A mutant F211 strain with high furfural tolerance was obtained from our previous study. Thus, its key tolerance mechanism was studied in the present study. The function of mutated genes in F211 was identified by funct...

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Autores principales:	Dongsheng Hu, Zhiquan Wang, Mingxiong He, Yuanyuan Ma
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Zymomonas mobilis furfural tolerance RNA-Seq functional investigation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ca8f83f216b644d2823483b1cfeb2afc
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spelling	oai:doaj.org-article:ca8f83f216b644d2823483b1cfeb2afc2021-11-11T05:56:05ZFunctional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F2111664-302X10.3389/fmicb.2021.736583https://doaj.org/article/ca8f83f216b644d2823483b1cfeb2afc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.736583/fullhttps://doaj.org/toc/1664-302XFurfural is a major inhibitor in lignocellulose hydrolysate for Zymomonas mobilis. A mutant F211 strain with high furfural tolerance was obtained from our previous study. Thus, its key tolerance mechanism was studied in the present study. The function of mutated genes in F211 was identified by functional complementation experiments, revealing that the improved furfural tolerance was resulted from the C493T mutation of the ZCP4_0270 gene promoting cell flocculation and the mutation (G1075A)/downregulation of ZCP4_0970. Comparative transcriptome analysis revealed 139 differentially expressed genes between F211 and the control, CP4, in response to furfural stress. In addition, the reliability of the RNA-Seq data was also confirmed. The potential tolerance mechanism was further demonstrated by functional identification of tolerance genes as follows: (I) some upregulated or downregulated genes increase the levels of NAD(P)H, which is involved in the reduction of furfural to less toxic furfuryl alcohol, thus accelerating the detoxification of furfural; (II) the mutated ZCP4_0270 and upregulated cellulose synthetase gene (ZCP4_0241 and ZCP4_0242) increased flocculation to resist furfural stress; (III) upregulated molecular chaperone genes promote protein synthesis and repair stress-damaged proteins; and (IV) transporter genes ZCP4_1623–1,625 and ZCP4_1702–1703 were downregulated, saving energy for cell growth. The furfural-tolerant mechanism and corresponding functional genes were revealed, which provides a theoretical basis for developing robust chassis strains for synthetic biology efforts.Dongsheng HuZhiquan WangZhiquan WangMingxiong HeYuanyuan MaYuanyuan MaFrontiers Media S.A.articleZymomonas mobilisfurfuraltoleranceRNA-Seqfunctional investigationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zymomonas mobilis furfural tolerance RNA-Seq functional investigation Microbiology QR1-502
spellingShingle	Zymomonas mobilis furfural tolerance RNA-Seq functional investigation Microbiology QR1-502 Dongsheng Hu Zhiquan Wang Zhiquan Wang Mingxiong He Yuanyuan Ma Yuanyuan Ma Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
description	Furfural is a major inhibitor in lignocellulose hydrolysate for Zymomonas mobilis. A mutant F211 strain with high furfural tolerance was obtained from our previous study. Thus, its key tolerance mechanism was studied in the present study. The function of mutated genes in F211 was identified by functional complementation experiments, revealing that the improved furfural tolerance was resulted from the C493T mutation of the ZCP4_0270 gene promoting cell flocculation and the mutation (G1075A)/downregulation of ZCP4_0970. Comparative transcriptome analysis revealed 139 differentially expressed genes between F211 and the control, CP4, in response to furfural stress. In addition, the reliability of the RNA-Seq data was also confirmed. The potential tolerance mechanism was further demonstrated by functional identification of tolerance genes as follows: (I) some upregulated or downregulated genes increase the levels of NAD(P)H, which is involved in the reduction of furfural to less toxic furfuryl alcohol, thus accelerating the detoxification of furfural; (II) the mutated ZCP4_0270 and upregulated cellulose synthetase gene (ZCP4_0241 and ZCP4_0242) increased flocculation to resist furfural stress; (III) upregulated molecular chaperone genes promote protein synthesis and repair stress-damaged proteins; and (IV) transporter genes ZCP4_1623–1,625 and ZCP4_1702–1703 were downregulated, saving energy for cell growth. The furfural-tolerant mechanism and corresponding functional genes were revealed, which provides a theoretical basis for developing robust chassis strains for synthetic biology efforts.
format	article
author	Dongsheng Hu Zhiquan Wang Zhiquan Wang Mingxiong He Yuanyuan Ma Yuanyuan Ma
author_facet	Dongsheng Hu Zhiquan Wang Zhiquan Wang Mingxiong He Yuanyuan Ma Yuanyuan Ma
author_sort	Dongsheng Hu
title	Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
title_short	Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
title_full	Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
title_fullStr	Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
title_full_unstemmed	Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211
title_sort	functional gene identification and corresponding tolerant mechanism of high furfural-tolerant zymomonas mobilis strain f211
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ca8f83f216b644d2823483b1cfeb2afc
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Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

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